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Rolling Circle Amplification on Biotin-Streptavidin Complexes Immobilized to Activated Cyclic Polyolefin Surfaces
Molecular Biology Research Laboratory, Boston University, Boston, USA; Department of Biomedical Engineering, Boston University, Boston, USA
Molecular Biology Research Laboratory, Boston University, Boston, USA; Department of Biomedical Engineering, Boston University, Boston, USA
Molecular Biology Research Laboratory, Boston University, Boston, USA; Department of Biomedical Engineering, Boston University, Boston, USA
Molecular Biology Research Laboratory, Boston University, Boston, USA; Department of Biomedical Engineering, Boston University, Boston, USA
Molecular Biology Research Laboratory, Boston University, Boston, USA; Department of Biomedical Engineering, Boston University, Boston, USA; Department of Biology, Boston University, Boston, USA
- 1 Molecular Biology Research Laboratory, Boston University, Boston, USA; Department of Biomedical Engineering, Boston University, Boston, USA
- 2 Molecular Biology Research Laboratory, Boston University, Boston, USA; Department of Biomedical Engineering, Boston University, Boston, USA
- 3 Molecular Biology Research Laboratory, Boston University, Boston, USA; Department of Biomedical Engineering, Boston University, Boston, USA
- 4 Molecular Biology Research Laboratory, Boston University, Boston, USA; Department of Biomedical Engineering, Boston University, Boston, USA
- 5 Molecular Biology Research Laboratory, Boston University, Boston, USA; Department of Biomedical Engineering, Boston University, Boston, USA; Department of Biology, Boston University, Boston, USA
Materials Sciences and Applications·Volume 04 (2013)·Pages 538–548·Published 3 September 2013·DOI10.4236/msa.2013.49066
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Abstract
Cyclic polyolefin (COP) is an inexpensive hydrophobic material with low auto-fluorescence, high light transmittance and thermal stability, broad chemical resistance and no non-specific protein binding. Here, the hydrophobic alkane COP was modified to have carbonyl functionalities through oxygen plasma and chemical etching treatments to increase use fulness for chemical and biochemical applications. Then, biotin-hydrazide was used to create biotinylated surfaces that bound streptavidin. A biotinylated target oligonucleotide was subsequently bound to the immobilized biotin - streptavidin and ligation mediated rolling circle amplification-based (L-RCA) SNP detection was demonstrated.
KeywordsCyclic PolyolefinLigation Mediated Rolling Circle AmplificationSNP Detection
- W. D. Niles and P. J. Coassin, “Cyclic Olefin Polymers: Innovative Materials for High-Density Multiwell Plates,” Assay and Drug Development Technologies, Vol. 6, No. 4, 2008, pp. 577-590. doi:10.1089/adt.2008.134
- M. M. Dudek, R. P. Gandhiraman, C. Volcke, A. A. Cafolla, S. Daniels and A. J. Killard, “Plasma Surface Modification of Cyclo-Olefin Polymers and Its Application to Lateral Flow Bioassays,” Langmuir, Vol. 25, No. 18, 2009, pp. 11155-11161. doi:10.1021/la901455g
- T. B. Stachowiak, D. A. Mair, T. G. Holden, L. J. Lee, F. Svec and J. M. Frechet, “Hydrophilic Surface Modification of Cyclic Olefin Copolymer Microfluidic Chips Using Sequential Photografting,” Journal of Separation Science, Vol. 30, No. 7, 2007, pp. 1088-1093. doi:10.1002/jssc.200600515
- Q. Cao, M. C. Kim and C. Klapperich, “Plastic Microfluidic Chip for Continuous-Flow Polymerase Chain Reaction: Simulations and Experiments,” Biotechnology Journal, Vol. 6, No. 2, 2011, pp. 177-184. doi:10.1002/biot.201000100
- Y. Fuchiwaki, H. Nagai, M. Saito and E. Tamiya, “Ultra-Rapid Flow-Through Polymerase Chain Reaction Microfluidics Using Vapor Pressure,” Biosensors and Bioelectronics, Vol. 27, No. 1, 2011, pp. 88-94. doi:10.1016/j.bios.2011.06.022
- A. Bhattacharyya and C. M. Klapperich, “Differential Gene Expression Using mRNA Isolated on Plastic Microfluidic Chips,” Conference Proceedings of IEEE Engineering in Medicine and Biology Society, Vol. 2009, 2009, pp. 1067-1070.
- M. Mahalanabis, J. Do, H. Almuayad, J. Y. Zhang and C. M. Klapperich, “An Integrated Disposable Device for DNA Extraction and Helicase Dependent Amplification,” Biosensors and Bioelectronics, Vol. 12, 2010, pp. 335-359. doi:10.1007/s10544-009-9391-8
- A. Bhattacharyya and C. M. Klapperich, “Thermoplastic Microfluidic Device for On-Chip Purification of Nucleic Acids for Disposable Diagnostics,” Analytical Chemistry, Vol. 78, No. 3, 2006, pp. 788-792. doi:10.1021/ac051449j
- J. Raj, G. Herzog, M. Manning, C. Volcke, B. D. MacCraith, S. Ballantyne, M. Thompson and D. W. Arrigan, “Surface Immobilisation of Antibody on Cyclic Olefin Copolymer for Sandwich Immunoassay,” Biosensors and Bioelectronics, Vol. 24, No. 8, 2009, pp. 2654-2658. doi:10.1016/j.bios.2009.01.026
- C. Volcke, R. P. Gandhiraman, V. Gubala, J. Raj, T. Cummins, G. Fonder, R. I. Nooney, Z. Mekhalif, G. Herzog, S. Daniels, D. W. Arrigan, A. A. Cafolla and D. E. Williams, “Reactive Amine Surfaces for Biosensor Applications, Prepared by Plasma-Enhanced Chemical Vapour Modification of Polyolefin Materials,” Biosensors and Bioelectronics, Vol. 25, No. 8, 2010, pp. 1875-1880. doi:10.1016/j.bios.2009.12.034