How Polydopamine Modulates Biological Responses to PTFE Prostheses
- 1 National Institute of Health and Medical Research, INSERM UMR_S 1121, 11 rue Humann, Strasbourg, France
- 2 Pediatric surgery Department, University Hospital of Strasbourg, Avenue de Moliere, Strasbourg, France
- 3 National Institute of Health and Medical Research, INSERM UMR_S 1121, 11 rue Humann, Strasbourg, France
- 4 National Institute of Health and Medical Research, INSERM UMR_S 1121, 11 rue Humann, Strasbourg, France
- 5 National Center of Scientific Research, CNRS UMR_7199, UMR_7199, University of Pharmacy of Strasbourg, 74 Route du Rhin-CS 60024, Illkirch Cedex, France
- 6 National Center of Scientific Research, CNRS UMR_7199, UMR_7199, University of Pharmacy of Strasbourg, 74 Route du Rhin-CS 60024, Illkirch Cedex, France
- 7 National Institute of Health and Medical Research, INSERM UMR_S 1121, 11 rue Humann, Strasbourg, France
- 8 National Institute of Health and Medical Research, INSERM UMR_S 1121, 11 rue Humann, Strasbourg, France
Abstract
Diaphragm repair after congenital diaphragmatic hernia is associated with hernia recurrence due to prosthesis failure. Expanded polytetrafluoroethylene (e-PTFE), a synthetic non-degradable biomaterial, is currently used for those diaphragmatic defect repairs. The drawback of e-PTFE is its poor wettability that leads to coating difficulties, bonding that could favor implant integration. However, polydopamine (PDA) can be deposited as well on organic as on inorganic substrates. Therefore, we assessed the biological responses of a clinically used e-PTFE biomaterial treated with PDA in two different manners: one impregnated with PDA and the other coated with a one side PDA film. Mechanical properties of the raw e-PTFE, the PDA soaked biomaterial and the PDA coated surface were characterized by colloidal probe atomic force microscopy. Behaviors of primary human fibroblasts and Wharton’s jelly stem cells were investigated by electron microscopy. Findings reveal that the mechanical properties at the microscopic scale are not modified by the PDA treatments. Cells spread onto both PDA functionalized substrates. In addition, microscopic observations disclose numerous focal cell contacts, evidencing cell attachment, and cytoplasmic projections particularly with the nanoscale PDA coating. Results clearly suggest that PDA in general but above all the PDA coating enhance cellular colonization of the implant material.
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