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Direct Solid-State Fermentation of Soybean Processing Residues for the Production of Fungal Chitosan by Mucor rouxii
Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, USA
Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, USA
Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, USA
Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, USA
Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, USA
Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, USA
Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, USA
- 1 Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, USA
- 2 Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, USA
- 3 Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, USA
- 4 Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, USA
- 5 Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, USA
- 6 Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, USA
- 7 Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, USA
Journal of Materials Science and Chemical Engineering·Volume 03 (2015)·Pages 11–21·Published 21 January 2015·DOI10.4236/msce.2015.32003
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Abstract
The feasibility of utilizing soybean-processing residues such as soybean meal and hulls as substrates for chitosan production by the fungus Mucor rouxii ATCC 24905 via solid-state fermentation (SSF) was investigated. The effects of the type of soybean-based substrate, length of cultivation period, substrate moisture content, substrate pH, incubation temperature and extraction conditions on chitosan yield were determined. The results showed that a maximum fungal chitosan yield of up to 3.44% by dry substrate weight (34.4 g/kg) could be achieved using a pure soybean meal substrate with an initial moisture content of 50% (w/w) and pH of 5 - 6 incubated for six days at 25°C. A more severe heat treatment (autoclaving vs. refluxing) resulted in higher chitosan extraction yields regardless of the strength of extraction reagents. Fourier transform infrared (FTIR) analysis of the fungal chitosan revealed its degree of deacetylation (DDA) to be between 55% and 60%.
KeywordsSolid-State FermentationFungal ChitosanBiopolymersBioconversionAgro-Industrial Residues
- Castro, S.P.M. and Paulín, E.G.L. (2012) Is Chitosan a New Panacea? Areas of Application. In: Karunaratne, D.N., Ed., The Complex World of Polysaccharides, ISBN: 978-953-51-0819-1, InTech. http://www.intechopen.com/books/the-complex-world-of-polysaccharides/is-chitosan-a-new-panacea-areas-of-application
- Kaur, S., and Dhillon, G.S. (2014) The Versatile Biopolymer Chitosan: Potential Sources, Evaluation of Extraction Methods and Applications. Critical Reviews in Microbiology, 2, 155-175. http://dx.doi.org/10.3109/1040841X.2013.770385
- Badawy, M.E.I. and Rabea, E.I. (2011) A Biopolymer Chitosan and Its Derivatives as Promising Antimicrobial Agents against Plant Pathogens and Their Applications in Crop Protection. International Journal of Carbohydrate Chemistry, 2011, Article ID: 460381, 29 p. http://dx.doi.org/10.1155/2011/460381
- Dutta, P.K., Dutta, J., and Tripathi, V.S. (2004) Chitin and Chitosan: Chemistry, Properties and Applications. Journal of Scientific & Industrial Research, 63, 20-31. http://nopr.niscair.res.in/handle/123456789/5397
- Bruck, W.M., Slater, J.W., and Carney, B.F. (2011) Chitin and Chitosan from Marine Organisms. In: Kim, S.K., Ed., Chitin, Chitosan, Oligosaccharides, and Their Derivatives: Biological Activities and Applications, CRC Press, Boca Raton, 11-24.
- Knezevic-Jugovic, Z., Petronijevic, Z., and Smelcerovic, A. (2011) Chitin and Chitosan from Microorganisms. In: Kim, S.K., Ed., Chitin, Chitosan, Oligosaccharides, and Their Derivatives: Biological Activities and Applications, CRC Press, Boca Raton, 25-36.
- Peter, M.G. (2002) Chitin and Chitosan in Fungi. Biopolymers Online, 6, 123-157. http://dx.doi.org/10.1002/3527600035.bpol6005
- White, S.A., Farina, P.R. and Fulton, I. (1979) Production and Isolation of Chitosan from Mucor rouxii. Applied and Environmental Microbiology, 38, 323-328.
- Tan, S.C., Tan, T.K., Wong, S.M. and Khorb, E. (1996) The Chitosan Yield of Zygomycetes at Their Optimum Harvesting Time. Carbohydrate Polymers, 30, 239-242. http://dx.doi.org/10.1016/S0144-8617(96)00052-5
- Pochanavanich, P. and Suntornsuk, W. (2002) Fungal Chitosan Production and Its Characterization. Letters in Applied Microbiology, 35, 17-21. http://dx.doi.org/10.1046/j.1472-765X.2002.01118.x
- McGahren, W.J., Perkinson, G.A., Growich, J.A., Leese, R.A. and Ellestad, G.A. (1984) Chitosan by Fermentation. Process Biochemistry, 19, 88-90.