Diacylglycerol kinase (DGK) is an enzyme that converts diacylglycerol to phosphatidic acid. Several DGK isoforms have been implicated in the pathogenesis of seizure, but the role of DGK β in seizure is unknown. In the present study, we investigated the involvement of DGK β in seizure using DGK β knockout (KO) mice. Seizures were more severe in DGK β KO mice than in wild-type (WT) mice after pentylenetetrazol (PTZ) treatment and after kainic acid treatment, but there were no differences in latency to seizure. The expression levels of DGK β in the hippocampal CA1, CA3, or DG areas did not differ between PTZ (60 mg/kg) treatment and saline treatment. There were fewer parvalbumin-positive interneurons in the hippocampal CA3 area in DGK β KO mice than in control WT mice, which might partly account for the increased seizure susceptibility displayed by DGK β KO mice. These results suggest that DGK β may play a pivotal role in the development of the relevant interneurons, and that on inherent deficiency of DGK β increases the animal ’ s sensitivity to seizure-inducing stimuli.
KeywordsDiacylglycerol KinaseSeizureParvalbumin
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