To Optimize the Therapeutic Dose and Time Window of Picroside II in Cerebral Ischemic Injury in Rats by Orthogonal Test
- 1 Department of Neurology, The Second Affiliated Hospital, Qingdao University Medical College, Qingdao, China;
- 2 Department of Neurology, The Second Affiliated Hospital, Qingdao University Medical College, Qingdao, China;
- 3 Department of Neurology, The Second Affiliated Hospital, Qingdao University Medical College, Qingdao, China;
- 4 Department of Neurology, The Second Affiliated Hospital, Qingdao University Medical College, Qingdao, China;
- 5 Institute of Inte- grative Medicine, The Second Affiliated Hospital, Qingdao University Medical College, Qingdao, China.
- 6 Institute of Inte- grative Medicine, The Second Affiliated Hospital, Qingdao University Medical College, Qingdao, China.
Abstract
The paper aims to optimize the therapeutic dose and time window of picroside I I by orthogonal test in cerebral ischemic injury in rats. The forebrain ischemia models were established by bilateral common carotid artery occlusion (BCCAO) methods. The successful models were randomly divided into sixteen groups according to orthogonal experimental de sign and treated by injecting picroside I I intraperitonenally at different ischemic time with different dose. The concen trations of neuron-specific enolase (NSE), neuroglial marker protein S100B and myelin basic protein (MBP) in serum were determined by enzyme linked immunosorbent assay to evaluate the therapeutic effect of picroside I I in cerebral ischemic injury. The results indicated that best therapeutic time window and dose of picroside I I in cerebral ischemic injury were ischemia 1.5 h with 20 mg/kg body weight according to the concentrations of NSE, S100B and MBP in se rum . It is concluded that according to the principle of lowest therapeutic dose with longest time window, the optimized therapeutic dose and time window are injecting picroside I I intraperitonenally with 20 mg/kg body weight at ischemia 1.5 h in cerebral ischemic injury in rats.
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