Effects of Methylprednisolone on the Expression and Activity of Calpain Following Ischemia-Reperfusion Spinal Cord Injury in Rats — Oak Academic Publishing
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Effects of Methylprednisolone on the Expression and Activity of Calpain Following Ischemia-Reperfusion Spinal Cord Injury in Rats
Guanghua Integrative Medicine Hospital, Changning District, Shanghai, China; School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, Shanghai, China
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School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, Shanghai, China
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Department of Orthopedics, Shanghai Changhai Hospital, Shanghai, China
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Department of Orthopedics, Shanghai Changhai Hospital, Shanghai, China
1 Guanghua Integrative Medicine Hospital, Changning District, Shanghai, China; School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, Shanghai, China
2 School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, Shanghai, China
3 Department of Orthopedics, Shanghai Changhai Hospital, Shanghai, China
4 Department of Orthopedics, Shanghai Changhai Hospital, Shanghai, China
The present study was undertaken to examine the effects of methylprednisolone on the expression and activity of calpain in spinal cord tissue following spinal cord ischemia-reperfusion injury in rats. Adult male Sprague-Dawley rats were subjected to sham operations, ischemia-reperfusion and vehicle treated, or ischemia-reperfusion with methylprednisolone administration after injury. The expression of calpain I in the injured segments of the spinal cord as well as the degradation of the 68 kD neurofilament protein (NFP), a calpain-specific substrate, was determined at 3 h, 24 h, 72 h and 7 days after reperfusion using immunohistochemical labeling and western blot analysis, respectively. Three hours after spinal cord reperfusion, calpain I-positive cells and NFP degradation products were evident. The number of positive cells and immunoreactivity increased with time and peaked at 72 h after reperfusion. In addition, the number of calpain I-positive cells and the abundance of NFP degradation products were significantly lower in the methylprednisolone group, compared with vehicle treated animals following ischemia-reperfusion injury. The results of this study suggest that methylprednisolone can inhibit the expression and degradation activity of calpain following ischemia-reperfusion injury, providing further insight into the therapeutic benefits of methylprednisolone treatment for spinal cord injury.
Bracken, M.B., Shepard, M.J., Collins, W.F., Holford, T.R., Young, W., Baskin, D.S., et al. (1992) Methylprednisolone or Naloxone Treatment after Acute Spinal Cord Injury: 1-Year Follow-Up Data: Results of the Second National Acute Spinal Cord Injury Study. Journal of Neurosurgery, 76, 23-31. http://dx.doi.org/10.3171/jns.1992.76.1.0023
Bracken, M.B., Shepard, M.J., Holford, T.R., Leo-Summers, L., Aldrich, E.F., Fazl, M., et al. (1997) Administration of Methylprednisolone for 24 or 48 Hours or Tirilazad Mesylate for 48 Hours in the Treatment of Acute Spinal Cord Injury. Results of the Third National Acute Spinal Cord Injury Randomized Controlled Trial. National Acute Spinal Cord Injury Study. JAMA, 277, 1597-1604. http://dx.doi.org/10.1001/jama.1997.03540440031029
Kazanci, A., Seckin, H., Karadeniz, U., Kazanci, D., Turan, S., Kazanci, B., et al. (2010) Comparison of the Effect of Mexiletine and Methylprednisolone on Neural Function and Histopathological Damage after Transient Spinal Cord Ischemia in Rabbits. Turkish Neurosurgery, 20, 43-49.
James, T., Matzelle, D., Bartus, R., Hogan, E.L. and Banik, N.L. (1998) New Inhibitors of Calpain Prevent Degradation of Cytoskeletal and Myelin Proteins in Spinal Cord in vitro. Journal of Neuroscience Research, 51, 218-222. http://dx.doi.org/10.1002/(SICI)1097-4547(19980115)51:2 3.0.CO;2-4
Ray, S.K., Matzelle, D.D., Wilford, G.G., Hogan, E.L. and Banik, N.L. (2001) Cell Death in Spinal Cord Injury (SCI) Requires de novo Protein Synthesis. Calpain Inhibitor E-64-d Provides Neuroprotection in SCI Lesion and Penumbra. Annals of the New York Academy of Sciences, 939, 436-449. http://dx.doi.org/10.1111/j.1749-6632.2001.tb03655.x
Sribnick, E.A., Matzelle, D.D., Banik, N.L. and Ray, S.K. (2007) Direct Evidence for Calpain Involvement in Apoptotic Death of Neurons in Spinal Cord Injury in Rats and Neuroprotection with Calpain Inhibitor. Neurochemical Research, 32, 2210-2216. http://dx.doi.org/10.1007/s11064-007-9433-7
Hill, C.E., Guller, Y., Raffa, S.J., Hurtado, A. and Bunge, M.B. (2010) A Calpain Inhibitor Enhances the Survival of Schwann Cells in vitro and after Transplantation into the Injured Spinal Cord. Journal of Neurotrauma, 27, 1685-1695. http://dx.doi.org/10.1089/neu.2010.1272
Martiñón, S. and Ibarra, A. (2008) Pharmacological Neuroprotective Therapy for Acute Spinal Cord Injury: State of the Art. Mini-Reviews in Medicinal Chemistry, 8, 222-230. http://dx.doi.org/10.2174/138955708783744056
Colak, A., Kaya, M., Karaoglan, A., Sagmanligil, A., Akdemir, O., Sahan, E., et al. (2009) Calpain Inhibitor AK 295 Inhibits Calpain-Induced Apoptosis and Improves Neurologic Function after Traumatic Spinal Cord Injury in Rats. Neurocirugia (Astur), 20, 245-254.
Zhang, S.X., Bondada, V. and Geddes, J.W. (2003) Evaluation of Conditions for Calpain Inhibition in the Rat Spinal Cord: Effective Postinjury Inhibition with Intraspinal MDL28170 Microinjection. Journal of Neurotrauma, 20, 59-67. http://dx.doi.org/10.1089/08977150360517182
Yu, C.G., Joshi, A. and Geddes, J.W. (2008) Intraspinal MDL28170 Microinjection Improves Functional and Pathological Outcome Following Spinal Cord Injury. Neurotrauma, 25, 833-840. http://dx.doi.org/10.1089/neu.2007.0490
Yu, C.G. and Geddes, J.W. (2007) Sustained Calpain Inhibition Improves Locomotor Function and Tissue Sparing Following Contusive Spinal Cord Injury. Neurochemical Research, 32, 2046-2053. http://dx.doi.org/10.1007/s11064-007-9347-4
Schwartz, S.M., Duffy, J.Y., Pearl, J.M., Goins, S., Wagner, C.J. and Nelson, D.P. (2003) Glucocorticoids Preserve Calpastatin and Troponin I during Cardiopulmonary Bypass in Immature Pigs. Pediatric Research, 54, 91-97. http://dx.doi.org/10.1203/01.PDR.0000065730.79610.7D
Banik, N.L., Powers, J.M. and Hogan, E.L. (1980) The Effects of Spinal Cord Trauma on Myelin. Journal of Neuropathology & Experimental Neurology, 39, 232-244. http://dx.doi.org/10.1097/00005072-198005000-00002
Banik, N.L., Hogan, E.L., Powers, J.M. and Whetstine, L.J. (1982) Degradation of Cytoskeletal Proteins in Experimental Spinal Cord Injury. Neurochemical Research, 7, 1465-1475. http://dx.doi.org/10.1007/BF00965089
Banik, N.L., Hogan, E.L. and McAlhaney, W.W. (1984) A Role for Calcium and Activation of Neutral Proteinase in Myelinolysis? Progress in Clinical Biological Research, 146, 145-152.
Banik, N.L., Hogan, E.L., Powers, J.M. and Smith, K.P. (1986) Proteolytic Enzymes in Experimental Spinal Cord Injury. Journal of the Neurological Sciences, 73, 245-256. http://dx.doi.org/10.1016/0022-510X(86)90149-8
Banik, N.L., Shields, D.C., Ray, S., et al. (1998) Role of Calpain in Spinal Cord Injury: Effects of Calpain and Free Radical Inhibitors. Annals of the New York Academy of Sciences, 844, 131-137. http://dx.doi.org/10.1111/j.1749-6632.1998.tb08228.x
Ray, S.K., Wilford, G.G., Matzelle, D.C., Davis, B., Matzelle, D., Wilford, G., et al. (1999) Calpeptin and Methylprednisolone Inhibit Apoptosis in Rat Spinal Cord Injury. Annals of the New York Academy of Sciences, 890, 261-269. http://dx.doi.org/10.1111/j.1749-6632.1999.tb08001.x
Samantaray, S., Das, A., Thakore, N.P., Matzelle, D.D., Reiter, R.J., Ray, S.K., et al. (2009) Therapeutic Potential of Melatonin in Traumatic Central Nervous System Injury. Journal of Pineal Research, 47, 134-142. http://dx.doi.org/10.1111/j.1600-079X.2009.00703.x
Koc, R.K., Akdemir, H., Karakucuk, E.I., Oktem, I.S. and Menku, A. (1999) Effect of Methylprednisolone, Tirilazad Mesylate and Vitamin E on Lipid Peroxidation after Experimental Spinal Cord Injury. Spinal Cord, 37, 29-32. http://dx.doi.org/10.1038/sj.sc.3100732
Bartholdi, D. and Schwab, M.E. (1995) Methylprednisolone Inhibits Early Inflammatory Processes But Not Ischemic Cell Death after Experimental Spinal Cord Lesion in the Rat. Brain Research, 672, 177-186. http://dx.doi.org/10.1016/0006-8993(94)01410-J
Kanellopoulos, G.K., Kato, H., Wu, Y. and Kouchoukos, N.T. (1997) Neuronal Cell Death in the Ischemic Spinal Cord: The Effect of Methylprednisolone. The Annals of Thoracic Surgery, 64, 1279-1285. http://dx.doi.org/10.1016/S0003-4975(97)00903-X
Posmantur, R.M., Zhao, X., Kampfl, A., Clifton, G.L. and Hayes, R.L. (1998) Immunoblot Analyses of the Relative Contributions of Cysteine and Aspartic Proteases to Neurofilament Breakdown Products Following Experimental Brain Injury in Rats. Neurochemical Research, 23, 1265-1276. http://dx.doi.org/10.1023/A:1020792132629
Hall, E.D. and Springer, J.E. (2004) Neuroprotection and Acute Spinalcord Injury: A Reappraisal. NeuroRx, 1, 80-100. http://dx.doi.org/10.1602/neurorx.1.1.80
Hall, E.D., Yonkers, P.A., Taylor, B.M. and Sun, F.F. (1995) Lack of Effect of Postinjury Treatment with Methylprednisolone or Tirilazad Mesylate on the Increase in Eicosanoid Levels in the Acutely Injured Cat Spinalcord. Journal of Neurotrauma, 12, 245-256. http://dx.doi.org/10.1089/neu.1995.12.245
Banik, N.L., Matzelle, D., Terry, E. and Hogan, E.L. (1997) A New Mechanism of Methylprednisolone and Other Corticosteroids Action Demonstrated in vitro: Inhibition of a Proteinase (Calpain) Prevents Myelin and Cytoskeletal Protein Degradation. Brain Research, 748, 205-210. http://dx.doi.org/10.1016/S0006-8993(96)01302-9
Stracher, A. (1999) Calpain Inhibitors as Therapeutic Agents in Nerve and Muscle Degeneration. Annals of the New York Academy of Sciences, 11, 52-59. http://dx.doi.org/10.1111/j.1749-6632.1999.tb08635.x
Kumar, R., Krause, G.S., Yoshida, H., Mori, K. and DeGracia, D.J. (2003) Dysfunction of the Unfolded Protein Response during Global Brain Ischemia and Reperfusion. Journal of Cerebral Blood Flow & Metabolism, 23, 462-471. http://dx.doi.org/10.1097/00004647-200304000-00010
Ray, S.K., Matzelle, D.D., Wilford, G.G., Hogan, E.L. and Banik, N.L. (2000) Increased Calpain Expression Is Associated with Apoptosis in Rat Spinal Cord Injury: Calpain Inhibitor Provides Neuroprotection. Neurochemical Research, 25, 1191-1198. http://dx.doi.org/10.1023/A:1007631826160
Saatman, K.E., Abai, B., Grosvenor, A., Vorwerk, C., Smith, D.H. and Meaney, D.F. (2003) Traumatic Axonal Injury Results in Biphasic Calpain Activation and Retrograde Transport Impairment in Mice. Journal of Cerebral Blood Flow & Metabolism, 23, 34-42. http://dx.doi.org/10.1097/00004647-200301000-00004
Bracken, M.B., Shepard, M.J., Collins, W.F., Holford, T.R., Young, W., Baskin, D.S., et al. (1990) A Randomized Controlled Trial of Methylprednisolone or Naloxone in the Treatment of Acute Spinal-Cord Injury. Results of the Second National Acute Spinal Cord Injury Study. New England Journal of Medicine, 322, 1405-1411. http://dx.doi.org/10.1056/NEJM199005173222001