Analgesic and Anti-Inflammatory Activity of Total Crude Leaf Extract of <i>Phytolacca dodecandra</i> in Wistar Albino Rats — Oak Academic Publishing
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Analgesic and Anti-Inflammatory Activity of Total Crude Leaf Extract of <i>Phytolacca dodecandra</i> in Wistar Albino Rats
Department of Pharmacology, School of Medicine, St Augustine International University, Kampala, Uganda
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Department of Medical Physiology, Makerere University College of Health Sciences, Kampala, Uganda
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Department of Biotechnical and Diagnostic Sciences, Makerere University College of Veterinary Medicine, Animal Resources and Biosecurity, Kampala, Uganda
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Department of Pharmacology & Therapeutics, Makerere University College of Health Sciences, Kampala, Uganda
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Department of Pharmacology & Therapeutics, Makerere University College of Health Sciences, Kampala, Uganda
1 Department of Pharmacology, School of Medicine, St Augustine International University, Kampala, Uganda
2 Department of Medical Physiology, Makerere University College of Health Sciences, Kampala, Uganda
3 Department of Biotechnical and Diagnostic Sciences, Makerere University College of Veterinary Medicine, Animal Resources and Biosecurity, Kampala, Uganda
4 Department of Pharmacology & Therapeutics, Makerere University College of Health Sciences, Kampala, Uganda
5 Department of Pharmacology & Therapeutics, Makerere University College of Health Sciences, Kampala, Uganda
B ackgroun d: Pain is a common problem encountered in daily life among humans and animal and it is commonly managed conventionally using modern medicines and traditionally by use of medicinal plants. Phytolacca dodecandra is a common herb in Uganda, used traditionally to alleviate pain and inflammatory conditions. However, its activity in alleviating pain and inflammatory diseases has not been fully scientifically evaluated. Aim of Study: To evaluate the analgesic and anti-inflammatory activity of total crude leaf extracts of P. dodecandra in Wistar albino rats. Materials and Methods: It was a laboratory-based experimental study. Acetic acid-induced writhing test was used to determine the analgesic activity and the histamine-induced paw edema formation for anti-inflammatory activity of the extract. Twenty eight animals were divided into 7 groups each with 4 rats and two (2) sets of data were obtained from each animal per group as per guidance of the refinement rule of 3Rs. For analgesic activity, Group I was dosed with 1.0ml of normal saline (negative control), group II, III, IV, V, VI were dosed with 1000.0, 600.0, 150.0, 40.0 and 5.0 mg/kg bwt of extract respectively. Group VII was dosed 10.0 mg/kg bwt diclofenac sodium (Na) drug (positive control). For anti-inflammatory activity, Group I was dosed with 1.0ml of normal saline (negative control), Group II, III, IV, V, VI were dosed with 1000.0, 600.0, 300.0, 40.0 and 5.0 mg/kg bwt of extract respectively. Group VII was dosed 10.0 mg/kg bwt diclofenac Na. The percentage mean paw volume inhibition and percentage writhing protection were determined. Results: The mean percentage paw volume inhibitions were 29.1%, 74.2% and 32.3% at doses of 5.0, 40.0 mg/kg bwt and 10.0 mg/kg bwt diclofenac Na respectively. The mean percentage writhing protection was 8.9%, 10.4%, 78.5% and 98.7% at doses of 150.0, 600.0, 1000.0 mg/kg of extract and 10.0 mg/kg bwt diclofenac Na respectively. Conclusions: Total crude leaf extracts of P. dodecandra has analgesic and anti-inflammatory activity that may be attributed to the presence of polyphenolic, saponins and many others phytochemicals that are present in the plant.
Chen, L., Deng, H., Cui, H., Fang, J., Zuo, Z., Deng, J., et al. (2018) Inflammatory Responses and Inflammation-Associated Diseases in Organs. Oncotarget, 9, 7204-7218. https://doi.org/10.18632/oncotarget.23208 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805548/pdf/oncotarget-09-7204.pdf
Pinho-Ribeiro, F.A., Verri, W.A.J. and Chiu, I.M. (2017) Nociceptor Sensory Neuron-Immune Interactions in Pain and Inflammation. Trends in Immunology, 38, 5-19. https://doi.org/10.1016/j.it.2016.10.001
Tugume, P., Kakudidi, E.K., Buyinza, M., Namaalwa, J., Kamatenesi, M., Mucunguzi, P.C. and Kalema, J. (2016) Ethnobotanical Survey of Medicinal Plant Species Used by Communities around Mabira Central Forest Reserve, Uganda. Journal of Ethnobiology and Ethnomedicine, 12, 1-28. https://doi.org/10.1186/s13002-015-0077-4
Namukobe, J., Kasenene, J.M., Kiremire, B.T., Byamukama, R., Kamatenesi-Mugisha, M., Krief, S., et al. (2011) Traditional Plants Used for Medicinal Purposes by Local Communities around the Northern Sector of Kibale National Park, Uganda. Journal of Ethnopharmacology, 36, 236-245. https://doi.org/10.1016/j.jep.2011.04.044 http://www.ethnopharmacologia.org/prelude2018/pdf/biblio-hn-31-namukobe.pdf
Inusa, A., Sanusi, S.B., Linatoc, A.C., Mainassara, M.M. and Awawu, J.J. (2018) Phytochemical Analysis and Antimicrobial Activity of Bitter Leaf (Vernonia amygdalina) Collected from Lapai, Niger State, Nigeria on Some Selected Pathogenic Microorganisms. Science World Journal, 13, 15-18. https://www.ajol.info/index.php/swj/article/download/183577/172933
Alara, O.R., Abdurahman, N.H., Ukaegbu, C.I. and Kabbashi, N.A. (2019) Extraction and Characterization of Bioactive Compounds in Vernonia amygdalina Leaf Ethanolic Extract Comparing Soxhlet and Microwave-Assisted Extraction Techniques. Journal of Taibah University for Science, 13, 414-422. https://doi.org/10.1080/16583655.2019.1582460
Atiku, I., Sule, M.I., Pateh, U.U., Musa, A.M., Ya’u, J., Sani, Y.M., et al. (2015) Phytochemical, Anti-Inflammatory and Analgesic Studies of the Crude Ethanolic Leaf Extract of Globimetularbrounii Van Tieghem (Family: Loranthaceae). Natural Products Chemistry & Research, 3, 1-4. https://doi.org/10.4172/2329-6836.1000193
Sdayria, J., Rjeibi, I., Feriani, A., Ncib, S., Bouguerra, W., Hfaiedh, N., et al. (2018) Chemical Composition and Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract of Euphorbia retusa in Mice. Pain Research and Management, 2018, Article ID: 4838413. https://doi.org/10.1155/2018/4838413
Leaf Extract
Esser, K.B., Semagn, K. and Wolde-Yohannes, L. (2003) Medicinal Use and Social Status of the Soap Berry Endod (Phytolacca dodecandra) in Ethiopia. Journal of Ethnopharmacology, 85, 269-277. https://doi.org/10.1016/S0378-8741(03)00007-2
PROTA (2019) Phytolacca dodecandra L’Hér. PROTA (Plant Resources of Tropical Africa/Resources végétales de l’Afrique tropicale), Wageningen. https://uses.plantnet-project.org/en/Phytolacca_dodecandra_(PROTA)
Zimudzi, C. (2007) Phytolacca dodecandra L’Hér. Record from Protabase. PROTA (Plant Resources of Tropical Africa), Wageningen. https://uses.plantnet-project.org/en/Phytolacca_dodecandra_(PROTA)
Zeleke, A.J., Shimo, B.A. and Gebre, D.Y. (2017) Larvicidal Effect of Endod (Phytolacca dodecandra) Seed Products against Anopheles arabiensis (Diptera: Culicidae) in Ethiopia. BMC Research Notes, 10, Article No. 449. https://doi.org/10.1186/s13104-017-2792-5
Mekonnen, N., Makonnen, E., Aklilu, N. and Ameni, G. (2012) Evaluation of Berries of Phytolacca dodecandra for Growth Inhibition of Histoplasma capsulatum var. farciminosum and Treatment of Cases of Epizootic Lymphangitis in Ethiopia. Asian Pacific Journal of Tropical Biomedicine, 2, 505-510. https://doi.org/10.1016/S2221-1691(12)60086-0
Ogutu, A.I., Lilechi, D.B., Mutai, C. and Bii, C. (2012) Phytochemical Analysis and Antimicrobial Activity of Phytolacca dodecandra, Cucumis aculeatus and Erythrina excelsa. International Journal of Biological and Chemical Sciences, 6, 692-704. https://doi.org/10.4314/ijbcs.v6i2.13
Matebie, W.A., Zhang, W. and Xie, G. (2019) Chemical Composition and Antimicrobial Activity of Essential Oil from Phytolacca dodecandra Collected in Ethiopia. Molecules, 24, 342. https://doi.org/10.3390/molecules24020342
Alemu, A., Tamiru, W., Nedi, T. and Shibeshi, W. (2018) Analgesic and Anti-Inflammatory Effects of 80% Methanol Extract of Leonotis ocymifolia (Burm. f.) Iwarsson Leaves in Rodent Models. Evidence-Based Complementary and Alternative Medicine, 2018, Article ID: 1614793. https://doi.org/10.1155/2018/1614793
Salma, B.S.G., Ul-Hasan, M.M., Ahmed, S. and Fatima, S.A. (2018) Analgesic and Anti-Inflammatory Effects of Phaseolus vulgaris L. Fixed Oil in Rodents. Journal of Basic & Applied Sciences, 14, 174-179. https://doi.org/10.6000/1927-5129.2018.14.26 https://pdfs.semanticscholar.org/659a/dd339357742a12fa87eabd9a1f7f3b1a77fb.pdf
Sun, K., Song, X., Jia, R., Yin, Z., Zou, Y., Li, L., et al. (2018) Evaluation of Analgesic and Anti-Inflammatory Activities of Water Extract of Galla chinensis in Vivo Models. Evidence-Based Complementary and Alternative Medicine, 2018, Article ID: 6784032. https://doi.org/10.1155/2018/6784032
Udegbunam, R., Udegbunam, S. and Anosa, G. (2013) Analgesic and Anti-Inflammatory Effects of Cyphostemma vogelii (Hook. f.) Desc. Root Extract in Mice. African Journal of Biotechnology, 12, 2288-2292. https://doi.org/10.5897/AJB12.2230 https://www.ajol.info/index.php/ajb/article/download/129625/19189
NIH (2011) Guide for the Care and Use of Laboratory Animals. 8th Edition, Institute for Laboratory Animal Research, Division on Earth and Life Studies, National Research Council of the National Academies Press, Washington DC, 1-246. https://grants.nih.gov/grants/olaw/guide-for-the-care-and-use-of-laboratory-animals.pdf
Ward, J.D. (2008) A Manual for Laboratory Animal Management: Manuals in Biomedical Research. World Scientific Publishing, Singapore, Vol. 5, 1-368. https://doi.org/10.1142/6682
Festing, M.F.W. and Altman, D.G. (2002) Guidelines for the Design and Statistical Analysis of Experiments Using Laboratory Animals. ILAR Journal, 43, 244-258. http://www.3rs-reduction.co.uk/assets/applets/Festing_Altman.pdf https://doi.org/10.1093/ilar.43.4.244
Borgi, W., Ghedira, K. and Chouchane, N. (2007) Anti-Inflammatory and Analgesic Activities of Zizyphus lotus Root Barks. Fitoterapia, 78, 16-19. https://doi.org/10.1016/j.fitote.2006.09.010
Gupta, M., Mazumder, U.K., Gomathi, P. and Selvan, V.T. (2006) Anti-Inflammatory Evaluation of Leaves of Plumeria acuminata. BMC Complementary and Alternative Medicine, 6, 36. https://doi.org/10.1186/1472-6882-6-36
Vos, C.M.F. and Kazan, K. (2016) Below Ground Defense Strategies in Plants: Signaling and Communication in Plants. Springer International Publishers, Berlin, 1-410. https://doi.org/10.1007/978-3-319-42319-7
Duraipandiyan, V., Ayyanar, M. and Ignacimuthu, S. (2006) Antimicrobial Activity of Some Ethnomedicinal Plants Used by Paliyar Tribe from Tamil Nadu, India. BMC Complementary and Alternative Medicine, 6, 35. https://doi.org/10.1186/1472-6882-6-35
Hostettmann, K., Marston, A., Ndjoko, K. and Wolfender, J.L. (2000) The Potential of African Plants as a Source of Drugs. Current Organic Chemistry, 4, 973-1010. https://doi.org/10.2174/1385272003375923
Pavlov, V.A., Wang, H., Czura, C.J., Friedman, S.G. and Tracey, K.J. (2003) The Cholinergic Anti-Inflammatory Pathway: A Missing Link in Neuroimmunomodulation. Molecular Medicine, 9, 125-134. https://doi.org/10.1007/BF03402177 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1430829/pdf/mol9p125.pdf
Zabrodskii, P.F. (2010) The Mechanisms of the Cholinergic Anti-Inflammatory Pathway after Acute Intoxication of Organophosphorus Compounds. BioMedical Journal of Scientific and Technical Research, 13, 9968-9972. https://doi.org/10.26717/BJSTR.2019.13.002399