New Method for Isolation of Naringin Compound from <i>Citrus maxima</i>
- 1 University of Mataram, Mataram, Indonesia
- 2 University of Mataram, Mataram, Indonesia
- 3 University of Mataram, Mataram, Indonesia
- 4 University of Mataram, Mataram, Indonesia
- 5 University of Mataram, Mataram, Indonesia
Abstract
This research aims to know the proper isolation method to isolate naringin compound from Citrus maxima for natural product chemistry laboratory. This natural product chemistry laboratory provide s students with the essential skills and knowledge required to perform the extraction, isolation, and structural identification. The isolation began with sample preparation, extraction with methanol, partition with n-hexane, purification by crystallization using isopropanol , by analyzing the purity with Thin Layer Chromatography (TLC) tested , and the presence of functional groups with Fourier Transform Infrared (FT-IR) spectrophotometer test. The result of FT-IR spectrophotometer analysis contains hydroxy group (-OH), aromatic cyclic groups (C=C), CH 2 , CH 3 , and ether groups.
- Hakim, A., Liliasari, Kadarohman, A. and Syah, Y.M. (2016) Making a Natural Product Chemistry Course Meaningful with a Mini Project Laboratory. Journal of Chemical Education, 93, 193-196. https://doi.org/10.1021/ed500930s
- Raharjo, T.J. (2013) Kimia Hasil Alam. Pustaka Pelajar, Yogyakarta.
- Hakim, A., Liliasari, Kadarohman, A. and Syah, Y.M. (2016) Effects of the Natural Product Mini Project Laboratory on the Students Conceptual Understanding. Journal of Turkish Science Education, 13, 27-36.
- Hakim, A., Liliasari, Kadarohman, A. and Syah, Y.M. (2016) Improvement of Student Critical Thinking Skills with the Natural Product Mini Project Laboratory Learning. Indonesian Journal of Chemistry, 16, 315-321. https://doi.org/10.22146/ijc.21149
- Hakim, A. and Jufri, A.W. (2017) Applications of Isolation and Structure Elucidation of Secondary Metabolites in Natural Product Chemistry Laboratory. In: Advances in Chemistry Research, NOVA Publisher, New York, 5.
- Sudto, K., Surachai, P. and Supason, W. (2009) An Efficient Method for the Large Scale Isolation of Naringin from Pomelo (Citrus grandis) Peel. International Journal of Food Science and Technology, 44, 1737-1742. https://doi.org/10.1111/j.1365-2621.2009.01989.x
- Hakim, A., Andayani, Y. and Rahayuan, B.D. (2018) Isolation of Ethyl P-Methoxy Cinnamate from Kaemferia galangal L. IOP Conference Series: Journal of Physics, 1095, Article ID: 012039. https://doi.org/10.1088/1742-6596/1095/1/012039
- Hakim, A. and Jufri, A.W. (2018) Natural Products Laboratory Project: Isolation and Structure Elucidation of Piperin from Piper nigrum and Andrographolide from Andrographis paniculata. Journal of Turkish Science Education, 15, 15-28.