Molecular Identification of <i>Alternaria alternate</i> (Fr.) Keissl. from the Leaf Blight Disease of <i>Centella asiatica</i> L. — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Molecular Identification of <i>Alternaria alternate</i> (Fr.) Keissl. from the Leaf Blight Disease of <i>Centella asiatica</i> L.
Department of Botany, Jahangirnagar University, Dhaka, Bangladesh
,
Department of Botany, Jahangirnagar University, Dhaka, Bangladesh
,
Department of Botany, Jahangirnagar University, Dhaka, Bangladesh
1 Department of Botany, Jahangirnagar University, Dhaka, Bangladesh
2 Department of Botany, Jahangirnagar University, Dhaka, Bangladesh
3 Department of Botany, Jahangirnagar University, Dhaka, Bangladesh
Centella asiatica (L.), frequently known as Thankuni, is an important ethnobotanical plant in Bangladesh. This study was conducted to evaluate the morphological characteristics, cultural factors and molecular identification of the causal agent of Alternaria leaf blight disease of C. asiatica . The potato dextrose agar (PDA) medium recorded the maximum mycelial growth (69 mm), followed by the yeast extract agar (YEA) medium, while the honey peptone agar (HPA) medium recorded the lowest growth (27 mm). The optimal pH and temperature for mycelial growth of Alternaria alternata were 6 and 30°C, respectively. Internal transcribed spacer (ITS) region of Alternaria alternata PCR products measured 558 bp and blast search showed 99% sequence similarity with Alternaria alternata species complex. To the best of our knowledge, Alternaria leaf blight disease caused by Alternaria alternata is the first record in Bangladesh.
Mohapatra, P., Ray, A., Sandeep, I.S., Nayak, S. and Mohanty, S. (2021) Tissue-Culture-Mediated Biotechnological Intervention in Centella asiatica: A Potential Antidiabetic Plant. In: Biotechnology of Anti-Diabetic Medicinal Plants, Springer, Berlin, 89-116. https://doi.org/10.1007/978-981-16-3529-8_4
Alqahtani, A. Cho, J.L., Wong, K.H., Li, K.M., Razmovski-Naumovski, V. and Li, G.Q. (2017) Differentiation of Three Centella Species in Australia as Inferred from Morphological Characteristics, ISSR Molecular Fingerprinting and Phytochemical Composition. Frontiers in Plant Science, 8, 19-28. https://doi.org/10.3389/fpls.2017.01980
Zahara, K., Bibi, Y. and Tabassum, S. (2021) Clinical and Therapeutic Benefits of Centella asiatica. Pure and Applied Biology (PAB), 3, 152-159. https://doi.org/10.19045/bspab.2014.34004
Zainol, N.A., Voo, S.C., Sarmidi, M.R. and Aziz, R.A. (2008) Profiling of Centella asiatica (L.) Urban Extract. Malaysian Journal of Analytical Science, 12, 322-327.
Dubey, R. and Pandey, A.K. (2008) Occurrence of Pseudocercospora centelli sp. Nov. Causing Leaf Spot Disease of Centella asiatica L. Indian Journal of Mycology and Plant Pathology, 38, 514-516.
Manoharachary, C., Kunwar, I.K. and Babu, K.S. (2003) A New Leaf Spot of Centella asiatica Caused by Cercospora centellae sp. nov. Journal of Mycology and Plant Pathology, 33, 271-273.
Akter, P., Khatun, S., Bhowmik, D.D., Neela, F.A. and Alam, N. (2022) Vegetative Growth and Molecular Identification of Fusarium equiseti Isolated from Wilt Disease of Centella asiatica L. in Bangladesh. American Journal of Plant Sciences, 13, 294-305. https://doi.org/10.4236/ajps.2022.132018
Shukla, S., Singh, P., Shukla, S., Ali, S. and Didwania, N. (2023) Scope of Onsite, Portable Prevention Diagnostic Strategies for Alternaria Infections in Medicinal Plants. Biosensors, 13, Article No. 701. https://doi.org/10.3390/bios13070701
Schoch, C.L., Seifert, K.A., Huhndorf, S., Robert, V., Spouge, J.L., Levesque, C.A., Chen, W., Fungal Barcoding Consortium, Fungal Barcoding Consortium Author List, Bolchacova, E. and Voigt, K. (2012) Nuclear Ribosomal Internal Transcribed Spacer (ITS) Region as a Universal DNA Barcode Marker for Fungi. Proceedings of the national academy of Sciences, 109, 6241-6246. https://doi.org/10.1073/pnas.1117018109
Sikder, M.M., Mallik, M.R.I. and Alam, N. (2019) Identification and in Vitro Growth Characteristics of Entomopathogenic Fungus-Aschersonia sp. in Bangladesh. Advances in Zoology and Botany, 7, 11-18. https://doi.org/10.13189/azb.2019.070102
Alam, N., Kim, J.H., Shim, M.J., Lee, U.Y. and Lee, T.S. (2010) Mycelial Propagation and Molecular Phylogenetic Relationships of Commercially Cultivated Agrocybecyl indracea Based on ITS Sequences and RAPD. Mycobiology, 38, 89-96. https://doi.org/10.4489/MYCO.2010.38.2.089
Alam, N., Shim, M.J., Lee, M.W., Shin, P.G., Yoo, Y.B. and Lee, T.S. (2009) Phylogenetic Relationship in Different Commercial Strains of Pleurotus nebrodensis Based on ITS Sequence and RAPD. Mycobiology, 37, 183-188. https://doi.org/10.4489/MYCO.2009.37.3.183
Tamura, K., Stecher, G., Peterson, D. Filipski, A. and Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30, 2725-2729. https://doi.org/10.1093/molbev/mst197
Humpherson‐Jones, F.M. and Phelps, K. (1989) Climatic Factors Influencing Spore Production in Alternaria brassicae and Alternaria brassicicola. Annals of Applied Biology, 114, 449-458. https://doi.org/10.1111/j.1744-7348.1989.tb03360.x
Mahmuda, N., Afroj, S., Bhowmik, D.D. and Alam, N. (2021) Cultural Conditions for Mycelial Growth and Molecular Identification of Mucor circinelloides Based on ITS Sequence. Jahangirnagar University Journal of Biological Sciences, 10, 25-30. https://doi.org/10.3329/jujbs.v10i1-2.60845
Sanjuti, S.A., Alam, N., Sarker, N.C., Amin, R. and Kanon, A.J. (2013) Cultural Conditions for Vegetative Growth and Random Amplification of Polymorphic DNA in Different Strains of Pleurotussajor-caju. Bangladesh Journal of Mushroom, 7, 21-28.
Ahmmed, S.M., Sikder, M.M., Sultana, A., Sultana, S. and Alam, N. (2020) First Report on Leaf Spot Disease of Aloe vera Caused by Alternaria alternata (Fr.) Keissler in Bangladesh. International Journal of Botany Studies, 5, 164-169.
Pradeep, F.S., Begam, M.S., Palaniswamy, M. and Pradeep, B.V. (2013) Influence of Culture Media on Growth and Pigment Production by Fusarium moniliforme KUMBF1201 Isolated from Paddy Field Soil. World Applied Sciences Journal, 22, 70-77.
Taware, M.R., Gholve, V.M., Wagh, S.S., Kuldhar, D.P., Pawar, D.V. and Chavan, A.A. (2014) Effect of Different Culture Media, Temperature, pH, Carbon and Nitrogen Sources on Mycelial Growth and Sporulation of Alternaria carthami Causing Alternaria Blight of Safflower. International Journal of Plant Protection, 7, 349-353. https://doi.org/10.15740/HAS/IJPP/7.2/349-353
Ahmmed, M.S., Nisha, F.A. and Alam, N. (2022) First Report on Rhizome Rot Disease of Curcuma longa Caused by Fusarium solani in Bangladesh. American Journal of Plant Sciences, 13, 506-516. https://doi.org/10.4236/ajps.2022.134033
Singh, D.B. (1980) Effect of Culture Media, pH and Temperature on Growth Behaviour of Alternaria brassicae and Drechslera graminea. Proceedings of the Indian National Science Academy B, 46, 393-396.
Hubballi, M., Nakkeeran, S., Raguchander, T., Anand, T. and Samiyappan, R. (2010) Effect of Environmental Conditions on Growth of Alternaria alternata Causing Leaf Blight of Noni. World Journal of Agricultural Sciences, 6, 171-177.
Ahmmed, S.M., Sikder, M.M., Sultana, A., Sultana, S. and Alam, N. (2021) First Report of Leaf Spot Disease of Aloe vera Caused by Fusarium oxyporum in Bangladesh. International Journal of Progressive Sciences and Technologies, 29, 251-260.
Alam, N. and Rahman, F. (2020) Vegetative Growth and Genetic Diversity in Different Strains of Pleurotus salmoneastramineus Based on PCR Polymorphism. Bangladesh Journal of Botany, 49, 125-134. https://doi.org/10.3329/bjb.v49i1.49119