A qualitative analysis of supercritical fluid extraction (SFE) of the medically useful compound, sage (Salvia officinalis) is presented in this study. Carbon dioxide was used as a supercritical solvent. Effects of operating conditions such as temperature, pressure, and extraction time were investigated. The effect of addition of cosolvents was also studied. The extracted useful compounds were identified and analyzed using GC-MS chromatography. The analysis of the extract obtained by SFE showed certain significant major compounds that are considered medically important. These major compounds are 1R-.alpha.-Pinene, D-Limonene, l-Camphene, and b-Pinene, and Eucalyptol. Although, there was no consistent trend with respect to the effect of the studied parameters on the composition of the extract, the selectivity of these major compounds are dependant on the operating conditions, manily the operating pressure. An increase in the pressure resulted in an increase in herb extraction yield; this is true for certain group of extracts, while the inverse is also true for other group of compounds. The effect of certain cosolvents on the extractability of sage showed a significant improvement of the yield of some particular constituents of sage.
University of Maryland (2010) Medical centre. http://www.umm.edu/altmed/articles/herbal-medicine-000351.htm
Laurence, M.H. and Christopher, J.M. (1998) Experimental organic chemistry: Principles and practice, Second Edition. Blackwell Science Ltd.
Durling, N.E., Catchpole, O.J., Grey, J.B., Webby, R.F., Mitchell, K.A. and Yeap, F.L. (2007) Extraction of phenolics and essential oil from dried sage (Salvia officinalis) using ethanol-water mixtures. Food Chemistry, 101, 1417-1424. doi:10.1016/j.foodchem.2006.03.050
Areias, F., Valentao, P., Andrade, P.B., Ferreres, F. and Seabra, R.M. (2000) Flavonoids and phenolic acids of sage: Influence of some agricultural factors. Journal of Agricultural and Food Chemistry, 48, 6081-6084. doi:10.1021/jf000440+
Guenther, E. (1974) The essential oils. Van Nostrand, New York.
Nassr-Allah, A., Aboul-Enein, A.M., Aboul-Enein, K.M., Lightfoot, D.A., Cocchetto, A. and El-Shem, H.A. (2009) Anti-cancer and anti-oxidant activity of some Egyptian medicinal plants. Journal of Medicinal Plants Research, 3, 799-808.
Jimenez-Carmona, M.M, Ubera, J.L. and Luque De Castro, M.D. (1999) Comparison of continuous subcritical water extraction and hydro distillation of marjoram essential oil. Journal of Chromatography, 855, 625-632. doi:10.1016/S0021-9673(99)00703-7
Ehlers, D., Nguyen, T., Quirin, K.W. and Gerard, D. (2001) Analysis of essential basil oils-CO2 extracts and steam-distilled oils. Deutsche Lebensmittel-Rundschau, 97, 245-250.
Singh, H., Hasan, M. and Kang, L.J. (2003) Supercritical carbon dioxide extraction of Sarawak black pepper oil. Internal Report, Department of Chemical Engineering, University of Malaya.
Langa, E., Della Porta, G., Palavra, A.M.F, Urieta, J.S. and Mainar A.M. (2009) Supercritical fluid extraction of Spanish sage es-sential oil: Optimization of the process parameters and modeling. The Journal of Supercritical Fluids, 49, 174-181. doi:10.1016/j.supflu.2008.12.007
Yepez, B., Espinosa, M. and Bolanos, L.G. (2002) Producing antioxidant fractions from herbaceous matrices by super-critical fluid extraction. Fluid Phase Equilibria, 194-197, 879-884. doi:10.1016/S0378-3812(01)00707-5
Chiew, W.P., May, C.Y., Ngan, M.A., Hock, C.C. (2005) Supercritical fluid extraction of palm carotenoids. American Journal of Environmental Sciences, 1, 264-269. doi:10.3844/ajessp.2005.264.269
Honye (2010) Honey Chemical Co. http://www.hongyechemical.com/e_pinene.htm
Sun, J.D. (2007) D-Limonene: Safety and clinical applications. Alternative Medicine Review, 12, 259-264.
Wikipedia (2010) Eucalyptus_oil. http://en.wikipedia.org/wiki/Eucalyptus_oil
Deba, F., Xuan, T.D., Yasuda M. and Tawata, S. (2008) Chemical composition and antioxidant, antibacterial and antifungal activities of the essential oils from Bidens pilosa Linn. var. Radiata. Food Control, 19, 346-352. doi:10.1016/j.foodcont.2007.04.011
Sonsuzer, S., Sahin, S., Yilmaz, L. (2004) Optimization of supercritical CO2 extraction of Thymbra spicata oil. Journal of Super-critical Fluids, 30, 189-199. doi:10.1016/j.supflu.2003.07.006
Menaker, A., Kravets, M., Koel, M. and Orav A. (2004) Identification and characterization of supercritical fluid extracts from herbs. Comptes Rendus Chimie, 7, 629-633. doi:10.1016/j.crci.2004.03.005
Marentis, R.T. (1988) Steps to developing a commercial supercritical carbon dioxide processing plant. In: Carpentier, B.A. and Sevenants, M.R., Eds., Supercritical Fluid Extraction and Chromatography, American Chemical Society, Washington DC. doi:10.1021/bk-1988-0366.ch007
Yang, Y., Gharaibeh, A., Hawthorne, S.B. and Miller, D.J. (1995) Combined temperature & modifier effects on supercritical CO2 extraction efficiencies of polycyclic aromatic hydrocarbons from environmental samples. Analytical Chemistry, 67, 641-646. doi:10.1021/ac00099a023
Kiriamiti, H.K., Rascol, E., Marty, A. and Condoret, J.S. (2002) Extraction rates of oil from high oleic sunflower seeds with supercritical carbon dioxide. Chemical Engineering and Processing, 41, 711-718. doi:10.1016/S0255-2701(01)00191-X
Span, R. and Wagner, W. (1996) A new equation of state carbon dioxide covering the region from triple-point temperature to 1100 K at pressure up to 800 MPa. Journal of Physical and Chemical Reference Data, 25, 1509- 1596. doi:10.1063/1.555991
Lucas, S., Calvo, M.P., Palencia, C. and Cocero, M.J. (2004) Mathematical model of supercritical CO2 adsorption on activated carbon: Effect of operating conditions and adsorption scale-up. Journal of Supercritical Fluids, 32, 193-201. doi:10.1016/j.supflu.2004.02.008
Baysal, T. and Starmans, D.A.J. (1999) Supercritical carbon dioxide extraction of carvone and limonene from caraway seed. Journal of Supercritical Fluids, 14, 225- 234. doi:10.1016/S0896-8446(98)00099-0
Jossi, J.A., Stiel, L.I. and Thodos, G. (1962) The viscosity of pure substances in the dense gaseous and liquid phases. AIChE Journal, 8, 59-62. doi:10.1002/aic.690080116
Roy, B.C., Goto, M., Kodama, A. and Hirose, T. (1996) Super-critical CO2 extraction of essential oils and cuticular waxes from peppermint leaves. Journal of Chemical Technology and Biotechnology, 67, 21-26. doi:10.1002/(SICI)1097-4660(199609)67:1 3.0.CO;2-0
Ge, F.H., Lin, X.X., Huan, X.F., Shi, Q., Liang, B., Li J. and Zhong, G. (2002) Study on extraction of active ingredients from Andrographis paniculata using orthogonal experiment with supercritical carbon dioxide. Journal of Chinese Medical Materials, 25, 101-102.
Fliur, Z., and Andrei, V.M. (2006) Use of monoterpenes, 3-carene and 2-carene, as synthons in the stereoselective synthesis of 2,2-dimethyl-1,3-disubstituted cyclopropanes. Tetrahedron, 62, 9-29. doi:10.1016/j.tet.2005.09.001