A sensitive, rapid and validated liquid chromatography ? tandem mass spectrometry (LC-MS-MS) method for determination of Mimosine in Mimosa pudica Linn
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Abstract
A rapid, sensitive and accurate liquid chroma-tographic tandem mass spectrometric method is described for the determination of Mimosine in Mimosa pudica Linn. whole plant powder. Mi-mosine was extracted from the plant using 1.0% HCl in water. The chromatographic separation was achieved using a Thermo Hypurity C18 (50 x 4.6 mm) 5.0 μ column interfaced with a triple quadrapole mass spectrometer. The mobile phase consisted of a mixture of Methanol: 10 mM ammonium formate buffer whose pH was ad-justed to 3.00 ± 0.05 with formic acid (80:20, v/v) and was delivered at a flow rate of 1.0 mL min-1. Electrospray ionization (ESI) source operated in the negative ion mode was used for the quantitation. Detection was performed using an Applied Biosystems Sciex API 3200 Mass spec-trometer. The method was found to be simple, precise, accurate, fast, specific and sensitive and can be used for routine quality control analysis of Mimosine in Mimosa pudica Linn.
- Sharma, S.K. and Wattal, B.L. (1979) Efficacy of some mucilaginous seeds as biological control agents against mosquito larvae. Journal of the Entomological Research, 3(2), 172-176.
- Vaidya, G.H. and Sheth, U.K. (1986) Mimosa pudica Linn., its medicinal value and pilot clinical use in patients with menorrhagia. Ancient Science of Life, 5(3), 156-160.
- Hemadri, K. and Rao, S.S. (1983) Leucorrhoea and Me-norrhagia. Tribal Medicine and Ancient Science of Life, 3(1), 40-41.
- Sharma, P.C., Yelna, M.B. and Dennis, T.J. (2001) Data-base on medicinal plants used in Ayurveda. Central Council for Research in Ayurveda & Siddha, India, 2, 369-379.
- Aalok, P.K. (1997) Lajjalu-an indispensable drug for blood pressure. Sachitra Ayurved, 50(1), 21-22.
- Zeitune, M.G. and Laguens, R.M., Inventors, Xl global corporation, assignee, use of mimosine or a derivative thereof for treating the cutaneous effects of psoriasis and related skin disorders, and cosmetic or pharmaceutical composition containing same. US patent 424725.
- (2006) The Mreck index. Mimosine, 14(6199), 1069.
- Bagul, M.S. and Rajani, M. (2005) Phytochemical evalua-tion of classical formulation: A case study. Indian Drugs, 42(11), 15-19.
- Debey, N., Debey, N., Mehta, R. and Saluja, A. (2009) Determination of psoralen and plumbagin from its poly-herbal oil formulation by an HPTLC. Journal of AOAC International, 92(3), 779-784.
- Nair, L.S., Menon, S.N., Shailajan, S., Baing, M.M. and Sane, R.T. (2007) Reverse phase high performance thin layer chromatographic quantification of Mimosine from whole plant of Mimosa pudica Linn. Journal of Planer Chromatography-Modern TLC, 20(1), 49-51.
- Puchala, R., Davis, J.J. and Sahlu, T. (1996) Determi-nation of mimosine and 3,4-dihydroxypyridine in milk and plasma of goats. Journal of Chromatography B: Biomedical Sciences and Applications, 685(2), 375- 378.
- Lalitha, K., Vargheese, C.M. and Balasubramanian, N., (1993) Spectrophotometric determination of Mimosine and e-hydroxy-4-(1H)-pyridone-the toxic principles of Leucaena leucocephala. Analytical Biochemistry, 213(1), 57-62.
- ICH-Q2A, (1994) Text on validation of analytical proce-dures. Proceedings of the International Conference on Harmonization of Technical Requirements for Registra-tion of Pharmaceuticals for Human Use, Geneva.