Diversity of mushrooms in Dry Dipterocarp forest at Phuphan National Park, Sakon Nakhon Province
- 1 Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani, Thailand;
- 2 Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani, Thailand;
Abstract
The purposes of this study were, 1) to study the variety of mushrooms grown in the Dry Dipterocarp forest during the year 2008-2009 by Releve method, 2) to study the relationship between ShoreasiamensisMiq. And Ectomycorrhizal of the Amanitaceae and the Belotaceae families, and 3) study the sequencesof DNAsinsome types of mushrooms in the Amanitaceae and the Balotaceae families by the PCR method. The findings of the study were as thefollowings: First, they were totally 34 types of mush-rooms found in Dry Dipterocarp forest at the Phuphan National Park during the studying period, 2008- 2009. There were 26 types found in both years Amanita hemibapha subsp. javanica Corner & Bas, A. princeps Corner & Bas, A. umbrinolutea (Secr. ex Gillet) Bataille, Termi-tomycesmicro- carpus (Berk. & Br.) R. Heim, T. perforans Heim, T. striatus f. griseus Heim, Boletellusananas (M. A. Curtis) Murrill, B. grisei-purpureus Cor., B. edulis Bull. ex Fr., B. luridus Schaeffer ex Fr., AlpovatrappeiFogel, Can-tharelluscibarius Fries, Craterellusaureus Berk. & Curis, Astraeushygrometricus (Pers.) Morgan, Lactariusaquifluus Peck, L. glaucescens Crossl., L. piperatus (Scop. ex Fr.) S. F. Gray, L. vellereus (Fr.) Fr., L. virescens Fr., R. densifolia (Secr.) Gill, R. emetica (Schaeff. & Fr.) S.F. Gray., R. xelempelina (Schaeff.) Fr., R. foetens (Pers.) Fr., R. rosacea (Pers. ex Secr.) Fries, R. violeipes Quél., R. virescens (Schaeff.) Fries. and those found only in 2009 were Bo-letuscurtisii M. A. Curtis, B. nobilis Peck, RussulaalboareolataHongo, R. cyanoxantha (Schaeff. ex Secr.) Fr. The mushrooms that had been found had significantly relations to the surrounding physical conditions. Second, there was a relationship between the Shoreasiamensis Miq. and Amanita princeps Cor.& Bas., Amanita hemibapha (Berk. & Br.) Sacc. subsp.javanica. Cor. & Bas., Boletus chrysenteron Bull., Boletus griseipurpureus Cor. and Heimiellarestipora (Pat & Bek.) Boedijn. at the intensity of spores 500, 5000, 50000 per each. From the SPSS for ANOVA analysis, the Shoreasiamensis Miq. had no relations to Amanita princeps Cor. & Bas., Amanita hemibapha (Berk. & Br.) Sacc. subsp. javanica. Cor. & Bas., Boletus chrysenteron Bull., Boletus grisei-purpureus Cor. and Heimiellarestipora (Pat & Bek.) Boedijn. in height and circumference growths of Shoreasiamensis Miq. seedlings at the intensity of spores 500, 5000 and 50,000 per each Shoreasiamensis Miq. at 95% level of confidence. Finally, by using the BLASTN computer program toexamine, compare and separate the differences 4 types of mushrooms’ DNA in the Amanitaceae and the Boletaceae families subjected to the PCR method, there were 4 findings i.e. 1342 DNA sbp. of Amanita princeps, 880 DNAs pb. of Boletusnobitis Peck, 1381 DNAs pb. of Boletus edulis Bull. exFr and 758 DNAs bp. of Heimiellaresipora (Pat & Baker).
- [1] Kirk, P.M., Camnon, P.E., Davidand J.C. and Stalpers J.A. (2001) Dictionary of the Fungi. 9th Edition, CABI Publishing, Wallingford.
- Smithe, F.B. (1975) Naturalist’s color guide. The American Museum of Natural History, New York.
- Corner, E.J.H. (1972) Boletus in Malaysia. Government Printing Office, Singapore.
- Moser, M. (1983) Key to Agarics and Boleti. Roger Phillips Publication.
- Ellis, M.B. and Ellis, J.P. (1990) Fungi without Gills (Hymenomycetes and Gasteromycetes) An identification Hand- book. Chapman and Hall, London.
- Lumyong, S., Sanmee, R. and Lumyong, P. (2007) Is large scale cultivation of boletes possible? Opera Mycilogica, 1, 34-37.
- Vilgalys, R. and Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology, 172, 4239-4246.
- White, T.J., Bruns, T., Lee, S. and Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White, T.J., Eds., PCR Protocols, A Guide to Methods and Applications, Academic Press, New York, 315-322.
- Brundrett, M., Bougher, N., Dell, B., Grove, T. and Malajczuk, N. (1996) Working with mycorrhizas in forestry and agriculture. Australian Centre for International Agricultural Research, Canberra.
- Felsenstein, J. (1985) Confidence limits on phylogenics: An approach using the bootstrap. Evolution, 6, 227-242.
- Hall, T.A. (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
- Huelsenbeck, J.P. and Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754-755. doi:10.1093/bioinformatics/17.8.754
- Mueller, G.M., Schmit, J.P., Huhndorf, S.M., Ryvarden, L., O’Dell, T.E., Lodge, D.J., Leacock, P.R., Mata, M.M., Umna, L., Wu, Q.X. and Czederpilt, D. (2004) Recommended protocols for sampling macrofungi. In: Mueller, G.M., Bills, G. and Foster, M.S., Eds., Biodiversity of fungi: Inventory and Monitoring Methods. Elsevier Academic Press, San Diego.
- Nylander, J.A.A. (2004) MrModeltest v2. Program distributed by the author. Uppsala University, Uppsala.
- Swofford, D.L. (2002) PAUP. Phylogenetis analysis using parsimony. Sinauer Associates, Sunderland. http://www.sinauer.com/detail.php?id=8060