Determination of bisphenol A and 4-nonylphenol in media samples for <i>in vitro</i> fertilization by high-performance liquid chromatography with tandem mass spectrometry
- 1 Division of Water and Food, Saitama Prefectural Institute of Public Health, Saitama, Japan
- 2 Division of Food Chemistry, Osaka Prefectural Institute of Public Health, Osaka, Japan
- 3 Division of Food Chemistry, Osaka Prefectural Institute of Public Health, Osaka, Japan
- 4 Division of Pharmacology, Aichi Medical University, Aichi, Japan
- 5 Department of Analytical Chemistry, Hoshi University, Tokyo, Japan
- 6 Tobu Hospital, Shizuoka, Japan; *Corresponding Author
Abstract
In vitro fertilization (IVF) is an important treatment for infertility, and in recent years, the delivery rate in IVF has increased markedly with the progress of reproductive medicine. However, chemical contaminants in IVF media have not been fully studied. A simple method using highperformance liquid chromatography coupled with tandem mass spectrometry was therefore investigated for quantitation of bisphenol A (BPA), 4nonylphenol (NP) and 4-n-nonylphenol ( n -NP) in IVF media. Media samples were processed using the solid-phase extraction method. The quantitation limits of BPA, NP, and n -NP were 0.5, 5 and 0.5 ng·ml - 1 , respectively, and the recoveries for BPA, NP and n-NP added to the media samples were above 90%. Commercially available media samples for IVF including 15 media for incubation of ova, 9 sperm washing media (SWM), 4 human serum albumin (HSA) samples and 2 substitute serum (SS) samples were examined, with 2 lots for each sample. NP was detected in 3 media for incubation of ova, 12 SWM, 7 HSA samples and 4 SS samples, in ranges of 6.3 14.5, 5.3 21.7, 5.5 164.0 and 9.0 19.0 ng·ml - 1 , respectively. BPA and n-NP were not detected in any of the samples.
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