Assessment on early blight of potato in order to compare the two methods<i> in vitro </i>using pathogenic fungi <i>Alternaria solani</i>

Abstract

Potato ( Solanum tuberosum ) early blight, caused by Alternaria solani is one of the most destructive fungal foliar diseases. This research was done in order to study methods comparison of evaluation by culture filtrate of A. solani in in vitro condition for selecting resistance cultivars to early blight. Plantlets of potato viruse free were obtained from the National plant gene bank of Iran, and were inoculated in vitro methods with a culture filtrate of A. solani . In in vitro selection by droplet of culture filtrate method, leaflet received a 10 μl droplet of the A. solani culture filtrate and in in vitro selection by direct using of culture filtrate method, plantlets were placed in test tubes that include 5 μl A. solani culture filtrate. The experimental design was factorial on basis of completely randomized design (CRD) with two factors, three replications and six genotypes. During droplet method assay, the A. solani symptoms appeared 1 - 2 days until 6 days and during direct method they appeared 2 - 3 days until 6 days. The AUDPC values were submitted to the analysis of varience (ANOVA) and AUDPC means were compared by using Duncan test (α = 0.01%). In each method, significant difference among potato cultivars was observed for disease to early blight (p < 0.01). Results show that casmos cultivar is susceptible for resistance to early blight and in vitro methods experiment had the same result.