Effect of Induced Mutation on Antioxidant Activity in <i>Ocimum basilicum</i> Linn
- 1 Department of Botany, University of Ghana, Accra, Ghana
- 2 Department of Pharmacognosy, Kwame Nkrumah University of Science Technology, Kumasi, Ghana
- 3 Department of Botany, University of Ghana, Accra, Ghana
- 4 Department of Botany, University of Ghana, Accra, Ghana
Abstract
Five batches from the same stock of seeds of Ocimum basilicum were irradiated at 5, 10, 15, 20 and 25 Gy, respectively using 60 C source. Methanolic leaf extracts of these samples and a control were evaluated for their antioxidant activity by the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging method using M 2 plants. All the methanolic extracts showed antioxidant activity. The IC 50 of the methanolic extracts of the six different treatments, control, 5 Gy, 10 Gy, 15 Gy, 20 Gy and 25 Gy, showed antioxidant activity with IC 50 values of 100, 90, 86, 61, 71 and 70 μg/ml, respectively. Three individual mutants, M-15-5, M-20-6 and M-15-4, had IC 50 values of 26, 30 and 40 μg/ml, respectively. These mutants were from the 15 Gy and 20 Gy treatments. From the results, it is confirmed that induced mutation can be employed to create variation in the levels of free radical scavenging activity in O. basilicum and can therefore serve as a tool for breeding for high levels of antioxidant activity in O. basilicum .
- Renaud, S.C., Gueguen, R., Schenker, J. and d’Houtaud, A. (1998) Alcohol and Mortality in Middle-Aged Men from Eastern France. Epidemiology, 9, 184-188. http://dx.doi.org/10.1097/00001648-199803000-00014
- Temple, N.J. (2000) Antioxidants and Disease: More Questions than Answers. Nutrition Research, 20, 449-459. http://dx.doi.org/10.1016/S0271-5317(00)00138-X
- Aqil, F., Ahmed, I. and Mehmood, Z. (2006) Antioxidant and Free Radical Scavenging Properties of Twelve Traditionally Used Indian Medicinal Plants. Turkish Journal of Biology, 30, 177-183.
- Zheng, W. and Wang, S.Y. (2001) Antioxidant Activity and Phenolic Compounds in Selected Herbs. Journal of Agricultural and Food Chemistry, 49, 5165-5170. http://dx.doi.org/10.1021/jf010697n
- Dragland, S., Sanoo, H., Wake, K., Holte, K. and Blomhoff, R. (2003) Several Culinary and Medicinal Herbs Are Important Sources of Dietary Antioxidants. Journal of Nutrition, 133, 1286-1290.
- Shan, B., Cai, Y.Z., Sun, M. and Corke, H. (2005) Antioxidant Capacity of 26 Spice Extracts and Characterization of Their Phenolic Constituents. Journal of Agricultural and Food Chemistry, 53, 7749-7759. http://dx.doi.org/10.1021/jf051513y
- Barros, L., Oliveira, S., Carvalho, A.M. and Ferreira, I.C.F.R. (2010) In Vitro Antioxidant Properties and Characterization in Nutrients and Phytochemicals of Six Medicinal Plants from the Portuguese Folk Medicine. Industrial Crops and Products, 32, 572-579. http://dx.doi.org/10.1016/j.indcrop.2010.07.012
- Dorman, H.J.D., Bachmayer, O., Kosar, M. and Hiltunen, R. (2004) Antioxidant Properties of Aqueous Extracts from Selected Lamiaceae Species Grown in Turkey. Journal of Agricultural and Food Chemistry, 52, 762-770. http://dx.doi.org/10.1021/jf034908v
- Kojo, A.I., Augustine, A.N.D. and Kofi, A. (2009) Screening of Some Ghanaian Medicinal Plants for Phenolic Compounds and Radical Scavenging Activities. Pharmacognosy Journal, 1, 206-211.