Optimization of Nitrogen, Phosphorus and Salt for Lipid Accumulation of Microalgae: Towards the Viability of Microalgae Biodiesel — Oak Academic Publishing
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Optimization of Nitrogen, Phosphorus and Salt for Lipid Accumulation of Microalgae: Towards the Viability of Microalgae Biodiesel
Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brasil
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Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brasil
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Medical Biochemistry Institute Leopoldo De Meis, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brasil
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Chemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brasil
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Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brasil
1 Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brasil
2 Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brasil
3 Medical Biochemistry Institute Leopoldo De Meis, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brasil
4 Chemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brasil
5 Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brasil
In recent years, microalgae biodiesel has attracted expressive attention and investment, once it was considered a potential resource for energy. Although the wide use of microalgae biodiesel is restricted by its high production cost. For cost-efficient and sustainable production of biodiesel from microalgae, a proper understanding of the variables and their impacts on physiology of the strains is required. In this study, a simple factorial design 23 was used to find optimal conditions for the cultivation of Ankistrodesmus sp. and Chlamydomonas sp. in batch culture. The three components considered were nitrate, phosphate and sodium chloride, used to assess the metabolic versatility of the strains in brackish conditions. The results showed that culture medium with 0.04 g·L?1 nitrate, 0.01 g·L?1 phosphate and 5.0 g·L?1 sodium chloride resulted to be the most effective condition to growth and fatty acids accumulation. Using this optimal condition, Ankistrodesmus sp. and Chlamydomonas sp. increased in 2.1 and 2.4 folds their fatty acids yield, respectively. Importantly, this protocol reduced 75% of the nitrate and phosphate concentrations of the original medium (ASM-1). Additionally, fatty acids analysis found that these strains were mainly constituted of C16-C18, in accordance with the requirements for biodiesel production. The simple factorial design applied here proved to be an important tool towards a better understanding of synergistic effects of tested factors on microalgae metabolism, and the resulting information could be used effectively to improve microalgae cultivation.
Bold, H.C. (1942) The Cultivation of Algae. Botanical Review, 8, 69-138. https://doi.org/10.1007/BF02879474
Pringsheim, E.G. (1946) The Biphasic or Soil-Water Culture Method for Growing Algae and Flagellata. Journal of Ecology, 33, 193-204. http://www.jstor.org/stable/2256465 https://doi.org/10.2307/2256465
Ma, F. and Hanna, M.A. (1999) Biodiesel Production: A Review. Bioresource Technology, 70, 1-15. https://doi.org/10.1016/S0960-8524(99)00025-5
Huang, G., Chen, F., Wei, D., Zhang, X. and Chen, G. (2010) Biodiesel Production by Microalgal Biotechnology. Applied Energy, 87, 38-46. https://doi.org/10.1016/j.apenergy.2009.06.016
Brennan, L. and Owende, P. (2010) Biofuels from Microalgae—A Review of Technologies for Production, Processing, and Extractions of Biofuels and Co-Products. Renewable & Sustainable Energy Reviews, 14, 557-577. https://doi.org/10.1016/j.rser.2009.10.009
Koller, M., Salerno, A., Tuffner, P., Koinigg, M., Bochzelt, H., Schober, S., et al. (2012) Characteristics and Potential of Micro Algal Cultivation Strategies: A Review. Journal of Cleaner Production, 37, 377-388. https://doi.org/10.1016/j.jclepro.2012.07.044
Sforza, E., Bertucco, A., Morosinotto, T. and Giacometti, G.M. (2012) Photobioreactors for Microalgal Growth and Oil Production with Nannochloropsis salina: From Lab-Scale Experiments to Large-Scale Design. Chemical Engineering Research and Design, 90, 1151-1158. https://doi.org/10.1016/j.cherd.2011.12.002
Venkata Mohan, S. and Devi, M.P. (2014) Salinity Stress Induced Lipid Synthesis to Harness Biodiesel during Dual Mode Cultivation of Mixotrophic Microalgae. Bioresource Technology, 165, 88-94. https://doi.org/10.1016/j.biortech.2014.02.103
Passarge, J., Hol, S., Escher, M. and Huisman, J. (2006) Competition for Nutrients and Light: Stable Coexistence, Alternative Stable States, or Competitive Exclusion? Ecological Monographs, 76, 57-72. https://doi.org/10.1890/04-1824
Wijffels, R.H., Kruse, O. and Hellingwerf, K.J. (2013) Potential of Industrial Biotechnology with Cyanobacteria and Eukaryotic Microalgae. Current Opinion in Biotechnology, 24, 405-413. https://doi.org/10.1016/j.copbio.2013.04.004
Smith, V.H., Sturm, B.S.M., de Noyelles, F.J. and Billings, S. (2010) The Ecology of Algal Biodiesel Production. Trends in Ecology & Evolution, 25, 301-309. https://doi.org/10.1016/j.tree.2009.11.007
Chen, G., Zhao, L. and Qi, Y. (2015) Enhancing the Productivity of Microalgae Cultivated in Wastewater toward Biofuel Production: A Critical Review. Applied Energy, 137, 282-291. https://doi.org/10.1016/j.apenergy.2014.10.032
Larsdotter, K. (2006) Wastewater Treatment with Microalgae—A Literature Review. Vatten, 62, 31-38.
Quiroz Arita, C.E., Peebles, C. and Bradley, T.H. (2015) Scalability of Combining Microalgae-Based Biofuels with Wastewater Facilities: A Review. Algal Research, 9, 160-169. https://doi.org/10.1016/j.algal.2015.03.001
Thompson, G. (1996) Lipids and Membrane Function in Green Algae. Biochimica et Biophysica Acta, 1302, 17-45. https://doi.org/10.1016/0005-2760(96)00045-8
Li, Z., Yuan, H., Yang, J. and Li, B. (2011) Optimization of the Biomass Production of Oil Algae Chlorella minutissima UTEX2341. Bioresource Technology, 102, 9128-9134. https://doi.org/10.1016/j.biortech.2011.07.004
Work, V.H., D’Adamo, S., Radakovits, R., Jinkerson, R.E. and Posewitz, M.C. (2012) Improving Photosynthesis and Metabolic Networks for the Competitive Production of Phototroph-Derived Biofuels. Current Opinion in Biotechnology, 23, 290-297. https://doi.org/10.1016/j.copbio.2011.11.022
Picardo, M.C., De Medeiros, J.L., Monteiro, J.G.M., Chaloub, R.M., Giordano, M. and De Queiroz Fernandes Araújo, O. (2013) A Methodology for Screening of Microalgae as a Decision Making Tool for Energy and Green Chemical Process Applications. Clean Technologies and Environmental Policy, 15, 275-291. https://doi.org/10.1007/s10098-012-0508-z
Reynolds, C.S. (2006) The Ecology of Phytoplankton. Cambridge University Press, Cambridge. https://doi.org/10.1017/CBO9780511542145
Sommer, U. (1999) A Comment on the Proper Use of Nutrient Ratios in Microalgal Ecology. Archiv fur Hydrobiologie, 146, 55-64. https://doi.org/10.1127/archiv-hydrobiol/146/1999/55
Miranda, C.T., Pinto, R.F., De Lima, D.V.N., Viegas, C.V., Costa, S.M. and Azevedo, S.M.F.O. (2015) Microalgae Lipid and Biodiesel Production: A Brazilian Challenge. American Journal of Plant Sciences, 6, 2522-2533. https://doi.org/10.4236/ajps.2015.615254
Gurr, M., Harwood, J. and Frayn, K. (2002) Lipid Biochemistry: An Introduction. Blackwell Science, Hoboken. https://doi.org/10.1002/9780470774366
Mata, T.M., Martins, A. and Caetano, N.S. (2010) Microalgae for Biodiesel Production and Other Applications: A Review. Renewable & Sustainable Energy Reviews, 14, 217-232. https://doi.org/10.1016/j.rser.2009.07.020
Spolaore, P., Joannis-Cassan, C., Duran, E. and Isambert, A. (2006) Commercial Applications of Microalgae. Journal of Bioscience and Bioengineering, 101, 87-96. https://doi.org/10.1263/jbb.101.87
Meng, X., Yang, J., Xu, X., Zhang, L., Nie, Q. and Xian, M. (2009) Biodiesel Production from Oleaginous Microorganisms. Renewable Energy, 34, 1-5. https://doi.org/10.1016/j.renene.2008.04.014
Rezanka, T., Lukavsky, J., Nedbalov, L. and Sigler, K. (2011) Effect of Nitrogen and Phosphorus Starvation on the Polyunsaturated Triacylglycerol Composition, Including Positional Isomer Distribution, in the alga Trachydiscus minutus. Phytochemistry, 72, 2342- 2351. https://doi.org/10.1016/j.phytochem.2011.08.017
Gao, Y., Yang, M. and Wang, C. (2013) Nutrient Deprivation Enhances Lipid Content in Marine Microalgae. Bioresource Technology, 147, 484-491. https://doi.org/10.1016/j.biortech.2013.08.066
Siaut, M., Cuiné, S., Cagnon, C., Fessler, B., Nguyen, M., Carrier, P., et al. (2011) Oil Accumulation in the Model Green Alga Chlamydomonas reinhardtii: Characterization, Variability between Common Laboratory Strains and Relationship with Starch Reserves. BMC Biotechnology, 11, 7. https://doi.org/10.1186/1472-6750-11-7
Takagi Karseno, M. and Yoshida, T. (2006) Effect of Salt Concentration on Intracellular Accu-mulation of Lipids and Triacylglyceride in Marine Microalgae Dunaliella Cells. Journal of Bioscience and Bioengineering, 101, 223-226. https://doi.org/10.1263/jbb.101.223
Ho, S.-H., Ye, X., Hasunuma, T., Chang, J.-S. and Kondo, A. (2014) Perspectives on Engineering Strategies for Improving Biofuel Production from Microalgae—A Critical Review. Biotechnology Advances, 32, 1448-1459. https://doi.org/10.1016/j.biotechadv.2014.09.002
Rawat, I., Ranjith Kumar, R., Mutanda, T. and Bux, F. (2013) Biodiesel from Microalgae: A Critical Evaluation from Laboratory to Large Scale Production. Applied Energy, 103, 444-467. https://doi.org/10.1016/j.apenergy.2012.10.004
Nicolaisen, O., Cuny, M. and Bolla, S. (2014) Factorial Experimental Designs as Tools to Optimize Rearing Conditions of Fish Larvae. Aquaculture, 422-423, 253-260. https://doi.org/10.1016/j.aquaculture.2013.12.018
Chen, J.-J., Li, Y.-R. and Lai, W.-L. (2014) Application of Experimental Design Methodology for Optimization of Biofuel Production from Microalgae. Biomass and Bioenergy, 64, 11-19. https://doi.org/10.1016/j.biombioe.2014.03.056
Kangatharalingam, N. (1993) Isolation and Verification of Anatoxin-A Producing Clones of Anabaena flosaquae (Lyngb.) de Breb. from a Eutrophic Lake. FEMS Microbiology Ecology, 12, 127-130. https://doi.org/10.1111/j.1574-6941.1993.tb00024.x
Bligh, E.G. and Dyer, W.J. (1959) A Rapid Method of Total Lipid Extraction and Purification. Canadian Journal of Biochemistry and Physiology, 37, 911-917. https://doi.org/10.1139/o59-099
Montgomery, D. (2009) Introduction to Statistical Quality Control. John Wiley & Sons, Hoboken.
Rambali, B., Baert, L. and Massart, D.L. (2001) Using Experimental Design to Optimize the Process Parameters in Fluidized Bed Granulation on a Semi-Full Scale. International Journal of Pharmaceutics, 220, 149-160. https://doi.org/10.1016/S0378-5173(01)00658-5
Harwood, J. (1989) Gas Chromatography and Lipids: A Practical Guide. Phytochemistry, 28, 3251-3252. https://doi.org/10.1016/0031-9422(89)80324-3
Xiong, W., Li, X., Xiang, J. and Wu, Q. (2008) High-Density Fermentation of Microalga Chlorella protothecoides in Bioreactor for Microbio-Diesel Production. Applied Microbiology and Biotechnology, 78, 29-36. https://doi.org/10.1007/s00253-007-1285-1
Su, C.H., Chien, L.J., Gomes, J., Lin, Y.S., Yu, Y.K. and Liou, J.S., et al. (2011) Factors Affecting Lipid Accumulation by Nannochloropsis oculata in a Two-Stage Cultivation Process. Journal of Applied Phycology, 23, 903-908. https://doi.org/10.1007/s10811-010-9609-4
Widjaja, A., Chien, C.C. and Ju, Y.H. (2009) Study of Increasing Lipid Production from Fresh Water Microalgae Chlorella vulgaris. Journal of the Taiwan Institute of Chemical Engineers, 40, 13-20. https://doi.org/10.1016/j.jtice.2008.07.007
Lv, J.M., Cheng, L.H., Xu, X.H., Zhang, L. and Chen, H.L. (2010) Enhanced Lipid Production of Chlorella vulgaris by Adjustment of Cultivation Conditions. Bioresource Technology, 101, 6797-6804. https://doi.org/10.1016/j.biortech.2010.03.120
Sharma, K.K., Schuhmann, H. and Schenk, P.M. (2012) High Lipid Induction in Microal-gae for Biodiesel Production. Energies, 5, 1532-1553. https://doi.org/10.3390/en5051532
Morowvat, M.H., Rasoul-Amini, S. and Ghasemi, Y. (2010) Chlamydomonas as a “New” Organism for Biodiesel Production. Bioresource Technology, 101, 2059-2062. https://doi.org/10.1016/j.biortech.2009.11.032
Merchant, S.S., Kropat, J., Liu, B., Shaw, J. and Warakanont, J. (2012) TAG, You’re It! Chlamydomonas as a Reference Organism for Understanding Algal Triacylglycerol Accumulation. Current Opinion in Biotechnolo-gy, 23, 352-363. https://doi.org/10.1016/j.copbio.2011.12.001
Fan, J., Andre, C. and Xu, C. (2011) A Chloroplast Pathway for the de Novo Biosynthesis of Triacylglycerol in Chlamydomonas reinhardtii. FEBS Letters, 585, 1985-1991. https://doi.org/10.1016/j.febslet.2011.05.018
Talebi, A.F., Mohtashami, S.K., Tabatabaei, M., Tohidfar, M., Bagheri, A., Zeinalabedini, M., et al. (2013) Fatty Acids Profiling: A Selective Criterion for Screening Microalgae Strains for Biodiesel Production. Algal Research, 2, 258-267. https://doi.org/10.1016/j.algal.2013.04.003
Díaz, G.C., Cruz, Y.R., Fortes, M.M. and Aranda, D.A.G. (2014) Primary Separation of Antioxidants (Unsaponifiables) the Wet Biomass Microalgae Chlamydomonas sp. and Production of the Bio-diesel. Natural Science, 6, 1210-1218. https://doi.org/10.4236/ns.2014.615108
Piorreck, M. and Pohl, P. (1984) Formation of Biomass, Total Protein, Chlorophylls, Lipids and Fatty Acids in Green and Blue-Green Algae during One Growth Phase. Phytochemistry, 23, 217-223. https://doi.org/10.1016/S0031-9422(00)80305-2
Converti, A., Casazza, A., Ortiz, E.Y., Perego, P. and Del Borghi, M. (2009) Effect of Temperature and Nitrogen Concentration on the Growth and Lipid Content of Nannochloropsis oculata and Chlorella vulgaris for Biodiesel Production. Chemical Engineering and Processing: Process Intensification, 48, 1146-1151. https://doi.org/10.1016/j.cep.2009.03.006
Reuss, N. and Poulsen, L.K. (2002) Evaluation of Fatty Acids as Biomarkers for a Natural Plankton Community. A Field Study of a Spring Bloom and a Post-Bloom Period off West Greenland. Marine Biology, 141, 423-434. https://doi.org/10.1007/s00227-002-0841-6
Lang, I., Hodac, L., Friedl, T. and Feussner, I. (2011) Fatty Acid Profiles and Their Distribution Patterns in Microalgae: A Comprehensive Analysis of More than 2000 Strains from the SAG Culture Collection. BMC Plant Biology, 11, 124. https://doi.org/10.1186/1471-2229-11-124