The utilization of ethnobotanical and phytochemical investigations in the discovery of novel medications is beneficial. Screening for phytochemicals is an important step in detecting the bioactive ingredients of medicinal plants which are used in conventional therapy. For the first time, 23 medicinal plants utilized in Saudi Arabian traditional therapy were examined. From August 2020 to July 2021, ethnobotanical fieldwork was conducted. There was some plant species identified, divided into pertinent families. Standard procedures were used to screen these medicinal plants for the occurrence of glycosides, alkaloids, saponins, resins, saponins, tannins, and flavonoids. Among the medicinal plants used, the most common phytochemicals were alkaloids (95.65%), glycosides (86.96%), saponin (82.61%), tannins (73.91%), flavonoids (56.52%), and resin (52.17%). The least widely distributed chemicals, on the other side, were resins. Trigonella foenum-graecum L., Pimpinella anisum L., and Cuminum cyminum L. seeds were shown to contain all six categories of secondary metabolites. The ethnographic importance of these medicinal plants is consistent with the content of secondary metabolites.
Al-Omar, M.S., Mohammed, H.A., Mohammed, S.A.A., et al. (2020) Anti-Microbial, Anti-Oxidant, and α-Amylase Inhibitory Activity of Traditionally-Used Medicinal Herbs: A Comparative Analyses of Pharmacology, and Phytoconstituents of Regional Halophytic Plants’ Diaspora. Molecules, 25, 5457. https://doi.org/10.3390/molecules25225457
Allah, K., Rehab, M., Yousef, M., et al. (2019) Utilization of Black Berry Juice to Reduce the Oxidative Stress in Rats Treated with Acrylamide. Asian Journal of Biological Sciences, 12, 9-16. https://doi.org/10.3923/ajbs.2019.9.16
Al-Qahtani, H., Alfarhan, A.H. and Al-Othman, Z.M. (2020) Changes in Chemical Composition of Zilla spinosa Forssk. Medicinal Plants Grown in Saudi Arabia in Response to Spatial and Seasonal Variations. Saudi Journal of Biological Sciences, 27, 2756-2769. https://doi.org/10.1016/j.sjbs.2020.06.035
Youssef, S.M., Sayed S.N., Mahmoud A.M.M., et al. (2020) Response of Stressed China Aster (Callistephus chinensis) cv. Kamini Plants to Foliar Application of Benzyladenine (BA) and Cycocel (CCC). The Seybold Report, 15, 30-42. https://doi.org/10.5281/zenodo.7350717
Karunamoorthi, K., Jegajeevanram, K., Vijayalakshmi, J. and Mengistie, E. (2013) Traditional Medicinal Plants: A Source of Phytotherapeutic Modality in Resource-Constrained Health Care Settings. Evidence-Based Complementary and Alternative Medicine, 18, 67-74. https://doi.org/10.1177/2156587212460241
Abdein, M.A. and Osman, A.K.E. (2020) Plant Diversity Assessment of Wadi Al-Hilali, Northern Border Region, Saudi Arabia. International Journal of Botany Studies, 5, 87-95.
Abdein, M.A. (2018) Genetic Diversity between Pumpkin Accessions Growing in the Northern Border Region in Saudi Arabia Based on Biochemical and Molecular Parameters. Egyptian Journal Botany, 58, 463-476. https://doi.org/10.21608/ejbo.2018.3612.1171
Mendoza, N. and Escamilla, E. (2018) Introduction to Phytochemicals: Secondary Metabolites from Plants with Active Principles for Pharmacological Importance. In: Asao, T. and Asaduzzaman, M., Eds., Phytochemicals: Source of Antioxidants and Role in Disease Prevention, IntechOpen, London, 25-47. https://doi.org/10.5772/intechopen.78226
Abualreish, M.J.A. and Abdein, M.A. (2014) The Analytical Applications and Biological Activity of Hydroxamic Acids. Journal of Advances in Chemistry, 10, 2118-2125. https://doi.org/10.24297/jac.v10i1.5587
Osman, A.K. and Abdein, M.A. (2019) Karyological and Molecular Studies between Six Species of Plantago in the Northern Border Region at Saudi Arabia. Journal of Taibah University for Science, 13, 297-308. https://doi.org/10.1080/16583655.2019.1571400
Osman, A.K.E. and Abdein, M.A. (2019) Floristic Diversity of Wadi Ar’ar, Saudi Arabia. Journal of Taibah University for Science, 13, 772-789. https://doi.org/10.1080/16583655.2019.1634177
Jamshidi-Kia, F., Lorigooini, Z. and Amini-Khoei, H. (2018) Medicinal Plants: Past History and Future Perspective. Journal of Herbmed Pharmacology, 7, 1-7. https://doi.org/10.15171/jhp.2018.01
Abdein, M.A., Wrda, H.N. and Osman, A.K. (2020) Genetic Characterization of Genus Tephrosia Pers. Based on Molecular Markers in KSA. International Journal of Botany Studies, 5, 203-209.
Gurib-Fakim, A. (2006) Medicinal Plants: Traditions of Yesterday and Drugs of Tomorrow. Molecular Aspects of Medicine, 27, 1-93. https://doi.org/10.1016/j.mam.2005.07.008
Rayan, A.M., Abdein, M.A. and Ibrahim, A.A. (2020) Associated Weeds of Some Agroecosystems in the Northern Border Region, KSA. International Journal of Botany Studies, 5, 345-351.
Stevanovic, Z.D., Stankovic, M.S., Stankovic, J., et al. (2019) Use of Halophytes as Medicinal Plants: Phytochemical Diversity and Biological Activity. In: Halophytes and Climate Change: Adaptive Mechanisms and Potential Uses, CABI, Wallingford, 343. https://doi.org/10.1079/9781786394330.0343
Abdel-Mageed, A.M., Osman, A.K.E., Awad, N.S. and Abdein, M.A. (2019) Evaluation of Antidiabetic Potentiality of Truffles and Balanites Aegyptiaca among Streptozotocin Induced Diabetic Rats. International Journal of Pharmaceutical Research & Allied Sciences, 8, 53-58.
Anywar, G. (2020) Historical Use of Toxic Plants. In: Mtewa, A.G., et al., Eds., Poisonous Plants and Phytochemicals in Drug Discovery, Wiley, Hoboken, 1-17. https://doi.org/10.1002/9781119650034.ch1
Egbuna, C., Kumar, S., Ifemeje, J.C., et al. (2019) Phytochemicals as Lead Compounds for New Drug Discovery. Elsevier, Amsterdam.
Alqethami, A., Hawkins, J.A. and Teixidor-Toneu, I. (2017) Medicinal Plants Used by Women in Mecca: Urban, Muslim and Gendered Knowledge. Journal of Ethnobiology and Ethnomedicine, 13, 62. https://doi.org/10.1186/s13002-017-0193-4
Jithesh, M.N., Prashanth, S.R., Sivaprakash, K.R. and Parida, A.K. (2006) Antioxidative Response Mechanisms in Halophytes: Their Role in Stress Defence. Journal of Genetics, 85, 237. https://doi.org/10.1007/BF02935340
Abd El-Moneim, D., Alqahtani, M.M., Abdein, M.A. and Germoush, M.O. (2020) Drought and Salinity Stress Response in Wheat: Physiological and TaNAC Genes Expression Analysis of Contrasting Egyptian Wheat Genotypes. Journal of Plant Biotechnology, 47, 1-14. https://doi.org/10.5010/JPB.2020.47.1.001
El-Refai, A.A., Sanad, M.I., Ramdan, A.-H.M. and Hikal, D.M. (2010) Antimicrobial Activity of Natural Anthocyanins and Carotenoids Extracted from Some Plants and Wastes. Journal of Food and Dairy Science, 1, 413-427. https://doi.org/10.21608/jfds.2010.82469
Al-Rowaily, S.L., Al-Dosari, D.H., Assaeed, A.M., et al. (2020) Native Perennial Plants Colonizing Abandoned Arable Fields in a Desert Area: Population Structure and Community Assembly. Agriculture, 10, 550. https://doi.org/10.3390/agriculture10110550
Modaihsh, A.S., Mahjoub, M.O., Sallam, A.S. and Ghoneim, A.M. (2015) Evaluation of Soil Degradation in Al-Kharj Centre, Saudi Arabia Using Remote Sensing. International Journal of Remote Sensing and Geoscience, 4, 1-7.
American Anthropological Association (2012) Statement on Ethics: Principles of Responsibility. http://www.aaanet.org/profdev/ethics
International Society of Ethnobiology (2006) ISE Code of Ethics (with 2008 Additions). https://www.ethnobiology.net/what-we-do/core-programs/ise-ethics-program/code-of-ethics/
Hikal, D.M. (2015) The Importance of Eggplant Peels Anthocyanins on Edible Oils as Natural Antioxidants. Australian Journal of Basic and Applied Sciences, 9, 491-496.
Yadav, R.N.S. and Agarwala, M. (2011) Phytochemical Analysis of Some Medicinal Plants. Journal of Phytology, 3, 10-14.
Thangaraj, P. (2016) Pharmacological Assays of Plant-Based Natural Products. Springer, Geneva.
Pandey, A. and Tripathi, S. (2014) Concept of Standardization, Extraction and Pre-Phytochemical Screening Strategies for Herbal Drug. Journal of Pharmacognosy and Phytochemistry, 2, 115-119.
Hikal, D.M. (2018) Antibacterial Activity of Piperine and Black Pepper Oil. Biosciences Biotechnology Research Asia, 15, 877-880. https://doi.org/10.13005/bbra/2697
Yadav, M., Chatterji, S., Gupta, S.K. and Watal, G. (2014) Preliminary Phytochemical Screening of Six Medicinal Plants Used in Traditional Medicine. International Journal of Pharmacy and Pharmaceutical Sciences, 6, 539-542.
Abdel-Mageed A.M., Muaz, M., Abdein, M.A. and Awad, N.S. (2021) Vitamin C Ameliorates the Cefepime-Induced Changes of Liver Enzymes, Histopathology and Proinflammatory Cytokines in Male Albino Rats. International Journal of Pharma and Bio Sciences, 11, 137-146.
Attia-Ismail, S.A. (2016) Plant Secondary Metabolites of Halophytes and Salt Tolerant Plants. In: El Shaer, H.M. and Squires, V.R., Eds., Halophytic and Salt-Tolerant Feedstuffs Impacts on Nutrition, Physiology and Reproduction of Livestock, CRC Press, Boca Raton, 348-357. https://doi.org/10.1201/b19862-24
Budan, A., Tessier, N., Saunier, M., et al. (2013) Effect of Several Saponin Containing Plant Extracts on Rumen Fermentation in Vitro, Tetrahymena pyriformis and Sheep Erythrocytes. Journal of Food, Agriculture & Environment, 11, 576-582.
Gutiérrez-Grijalva, E.P., López-Martínez, L.X., Contreras-Angulo, L.A., et al. (2020) Chapter 5. Plant Alkaloids: Structures and Bioactive Properties. In: Swamy, M.K., Ed., Plant-Derived Bioactives: Chemistry and Mode of Action, Springer, Berlin, 85-117. https://doi.org/10.1007/978-981-15-2361-8_5
Onaolapo, A.Y. and Onaolapo, O.J. (2019) Herbal Beverages and Brain Function in Health and Disease. In: Grumezescu, A.M. and Holban, A.M., Eds., Functional and Medicinal Beverages, Academic Press, Cambridge, 313-349. https://doi.org/10.1016/B978-0-12-816397-9.00009-1
González, M.G., Cabanelas, J.C. and Baselga, J. (2012) Applications of FTIR on Epoxy Resins—Identification, Monitoring the Curing Process, Phase Separation and Water Uptake. In: Theophanides, T., Ed., Infrared Spectroscopy—Materials Science, Engineering and Technology, IntechOpen, London, 261-284. https://doi.org/10.5772/36323
Pal, S. and Saha, C. (2014) A Review on Structure-Affinity Relationship of Dietary Flavonoids with Serum Albumins. Journal of Biomolecular Structure and Dynamics, 32, 1132-1147. https://doi.org/10.1080/07391102.2013.811700
Alqahtani, M.M., Abdein, M.A. and Abou El-Leel, O.F. (2020) Morphological and Molecular Genetic Assessment of Some Thymus Species. Biosciences Biotechnology Research Asia, 17, 103-113. https://doi.org/10.13005/bbra/2815
Galvano, F., La Fauci, L., Lazzarino, G., et al. (2004) Cyanidins: Metabolism and Biological Properties. The Journal of Nutritional Biochemistry, 15, 2-11. https://doi.org/10.1016/j.jnutbio.2003.07.004
Zhou, Q., Liang, D., Deng, A., et al. (2013) Antitussive, Expectorant and Bronchodilating Effects of Ethanol Extract of Sorghum bicolor (L.) Moench Roots. Journal of Ethnopharmacology, 149, 297-302. https://doi.org/10.1016/j.jep.2013.06.038
Hikal, D.M., Awad, N.S. and Abdein, M.A. (2017) The Anticancer Activity of Cashew (Anacardium occidentale) and Almond (Prunus dulcis) Kernels. Advances in Environmental Biology, 11, 31-41.
Al-Harbi, N.A., Awad, N.S., Alsberi, H.M. and Abdein, M.A. (2019) Apoptosis Induction, Cell Cycle Arrest and in Vitro Anticancer Potentiality of Convolvulus spicatus and Astragalus vogelii. World Journal of Environmental Biosciences, 8, 69-75.
Alhaithloul, H.A.S., Abdein, M.A. and Awad, N.S. (2022) Anticancer Effect of Citrullus colocynthis and Capparis spinosa against Human Cervix and Hepatocellular Cancer Cell Lines. Ecology, Environment and Conservation, 28, S586-S596. https://doi.org/10.53550/EEC.2022.v28i01s.081
Ghazzawy, H.S., Gouda, M.M., Awad, N.S., et al. (2022) Potential Bioactivity of Phoenix dactylifera Fruits, Leaves, and Seeds against Prostate and Pancreatic Cancer Cells. Frontiers in Nutrition, 9, Article ID: 998929. https://doi.org/10.3389/fnut.2022.998929
Kabera, J.N., Semana, E., Mussa, A.R. and He, X. (2014) Plant Secondary Metabolites: Biosynthesis, Classification, Function and Pharmacological Properties. Journal of Pharmacy and Pharmacology, 2, 377-392.
Alhaithloul, H.A. (2019) Environmental and Genetic Diversity of Rangeland Plant Species in Saudi Arabia. World Journal of Environmental Biosciences, 8, 57-66.
Khanbabaee, K. and van Ree, T. (2001) Tannins: Classification and Definition. Natural Product Reports, 18, 641-649.
Kareru, P.G., Keriko, J.M., Gachanja, A.N. and Kenji, G.M. (2008) Direct Detection of Triterpenoid Saponins in Medicinal Plants. African Journal of Traditional, Complementary, and Alternative Medicines, 5, 56-60. https://doi.org/10.4314/ajtcam.v5i1.31257
Al-Harbi, N.A., Al Attar, N.M., Hikal, D.M., et al. (2021) Evaluation of Insecticidal Effects of Plants Essential Oils Extracted from Basil, Black Seeds and Lavender against Sitophilus oryzae. Plants, 10, 829. https://doi.org/10.3390/plants10050829
Patel, K., Gadewar, M., Tripathi, R., et al. (2012) A Review on Medicinal Importance, Pharmacological Activity and Bioanalytical Aspects of Beta-Carboline Alkaloid ‘‘Harmine’’. Asian Pacific Journal of Tropical Biomedicine, 2, 660-664. https://doi.org/10.1016/S2221-1691(12)60116-6
Arafat, S.M., Basuny, A.M. and Hikal, D.M. (2021) Production of Cocoa Butter Substitute from Extra Virgin Olive Oil Rich in Omega-9 and Polyphenols. Food and Nutrition Sciences, 12, 494-508. https://doi.org/10.4236/fns.2021.126046
Basuny, A.M., Arafat, S.M. and Hikal, D.M. (2021) Chia (Salvia hispanica L.) Seed Oil Rich in Omega-3 Fatty Acid: A Healthy Alternative for Milk Fat in Ice Milk. Food and Nutrition Sciences, 12, 479-493. https://doi.org/10.4236/fns.2021.126037
Chung, K.T., Wong, T.Y., Wei, C.I., et al. (1998) Tannins and Human Health: A Review. Critical Reviews in Food Science and Nutrition, 38, 421-464. https://doi.org/10.1080/10408699891274273
Ragab, W.S., Gomah, N.H. and Abdein, M.A. (2020) Biological Control of Mold and Mycotoxin Contaminations in Food and Dairy Products. International Journal of Biology, Pharmacy and Allied Sciences, 9, 1128-1145. https://doi.org/10.31032/IJBPAS/2020/9.5.5082
El Aziz, M.M.A., Ashour, A.S. and Melad, A.S.G. (2019) A Review on Saponins from Medicinal Plants: Chemistry, Isolation, and Determination. Journal of Nanomedicine Research, 8, 282-288. https://doi.org/10.15406/jnmr.2019.07.00199
Habeballa, R.S., Ahmedani, E.I., Awad, N.S. and Abdein, M.A. (2020) In Vitro Antiviral Activity of Illicium verum and Zingiber officinale Ethanolic Extracts. Medical Science, 24, 3469-3480.
Parimal, K., Khale, A. and Pramod, K. (2011) Resins from Herbal Origin and a focus on Their Applications. International Journal of Pharmaceutical Sciences and Research, 2, 1077.
Alhaithloul, H.A.S., Attia, M.S. and Abdein, M.A. (2019) Dramatic Biochemical and Anatomical Changes in Eggplant Due to Infection with Alternaria solani Causing Early Blight Disease. International Journal of Botany Studies, 4, 55-60. http://www.botanyjournals.com/archives/2019/vol4/issue5/4-5-13
Loay, A.A., Rabie, M.M., Alhaithloul, H.A.S., et al. (2021) On the Biochemical and Physiological Responses of “Crimson Seedless” Grapes Coated with an Edible Composite of Pectin, Polyphenylene Alcohol, and Salicylic Acid. Horticulturae, 7, 498. https://doi.org/10.3390/horticulturae7110498
Loay, A.A., Elgammal, R.E., Alhaithloul, H.A.S., et al. (2021) Enhance Fruit Ripening Uniformity and Accelerate the Rutab Stage by Using ATP in “Zaghloul” Dates during the Shelf Life. Foods, 10, 2641. https://doi.org/10.3390/foods10112641
Taher, M.A., Loay, A.A., et al. (2022) Impacts of Gum Arabic and Polyvinylpyrrolidone (PVP) with Salicylic Acid on Peach Fruit (Prunus persica) Shelf Life. Molecules, 27, 2595. https://doi.org/10.3390/molecules27082595
El-Gioushy, S.F., Abdelkader, M.F.M., et al. (2022) The Effects of a Gum Arabic-Based Edible Coating on Guava Fruit Characteristics during Storage. Coatings, 12, 90. https://doi.org/10.3390/coatings12010090