Molecular Characterization of Aerobic Heterohophic Bacteria Isolated from Petroleum Hydrocarbon Polluted Brackish Waters of Bodo Creeks, Rivers State Nigeria — Oak Academic Publishing
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Molecular Characterization of Aerobic Heterohophic Bacteria Isolated from Petroleum Hydrocarbon Polluted Brackish Waters of Bodo Creeks, Rivers State Nigeria
Department of Microbiology, University of Port Harcourt, Choba, Nigeria
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Department of Microbiology, University of Port Harcourt, Choba, Nigeria
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Department of Microbiology, University of Port Harcourt, Choba, Nigeria
1 Department of Microbiology, University of Port Harcourt, Choba, Nigeria
2 Department of Microbiology, University of Port Harcourt, Choba, Nigeria
3 Department of Microbiology, University of Port Harcourt, Choba, Nigeria
Surface water sources in the oil producing Niger Delta region of Nigeria are highly susceptible to pollution by petroleum hydrocarbons and so it is important to understand the microbial diversity of such ecosystems. Water and sediment samples were collected between April-August, 2013 from Bodo creeks and taken to Environmental Microbiology laboratory of University of Portharcourt for analysis. A total of thirty aerobic heterotrophic bacterial strains isolated ranged from 3.0 - 7.0 × 10 4 cfu for surface water and 1.6 - 5.6 × 10 4 cfu for sediment samples of Bodo creek using serial dilution and spread plate technique. Pure cultures of bacteria were obtained on the basis of their morphological characteristics and subjected to biochemical tests and further classified on the basis of 16S rRNA gene sequence analysis. The DNA was isolated from size fractionated samples and the diversity of bacteria in each fraction was studied using PCR amplification of partial 16S rRNA. The sequences were submitted to NCBIGen bank for identification and assigning of accession numbers. The isolated aerobic heterotrophic bacteria belong to the families of Enterobacteriaceae, Bacilliceae, Alcaligenaceae, Pseudomonadaceae, Flavobactericeae and Planococcaceae.
Kirchman, D.L. (2008) Microbial Ecology of the Oceans. 2nd Edition, John Wiley and Sons Inc., USA. http://dx.doi.org/10.1002/9780470281840
Alexopoulos, A., Plessas, S. and Bezitzoglout, E. (2013) Water Microbial Ecology. An Overview. EOLSS, Orestiada, Greece.
Stager, C.E. and Davis, J.R. (1992) Automated Systems for Identification of Microorganisms. Clinical Microbiology Reviews, 5, 302-327.
Drancourt, M., Bollet, C., Cartioz, A., Martelin, R., Ayial, J.P. and Raoult, D. (2000) 16S Ribosomal DNA Sequence Analysis of a Large Collection of Environmental and Clinical Unidentifiable Bacterial Isolates. Journal of Clinical Microbiology, 38, 1031-1035.
Hagstrom, A., Pomier, T., Rohwer, F., Simi, K., Stolte, W., Sversson, D. and Zweifell, L. (2002) Use of 16S Ribosomal DNA for Delineation of Marine Bacterioplankton Species. Applied Environmental Microbiology, 68, 3628-3633. http://dx.doi.org/10.1128/AEM.68.7.3628-3633.2002
Halpern, B.S., Walbridge, S., Selkpe, K.A., Kappel, C.V., Michel, F., Dagrosa, C., Bruno, J.F., Casey, K.S., Ebert, C., Fox, H.E., Fujita, R., Heinemann, D., Lenihan, H.S., Madin, E.M., Perry, M.T., Selig, E.R., Spalding, M., Steneck, R. and Watson, R.A. (2008) A Global Map of Human Impact on Marine Ecosystem. Science, 319, 948-952. http://dx.doi.org/10.1126/science.1149345
McGenity, T.J., Folwell, B.D., Mckew, R.A. and Sanni, G.O. (2012) Marine Crude Oil Biodegradation: A Central Role for Interspecies Interactions. Aquatic Biosystems, 8, 10. http://dx.doi.org/10.1186/2046-9063-8-10
Agnieszka, C., Katarzyna, P., Kosakowska, A. and Kurlenda, J. (2006) Heterptrophic Bacteria from Brakish Water of the Southern Baltic Sea: Biochemical and Molecular Identification and Characterization. Oceanologia, 48, 525-543.
Cho, J.-C. and Giovannoni, S.J. (2003) Fulvimarina pelagi gen. nov. sp. nov., a Marine Bacterium That Forms a Deep Evolutionary Lineage Descent in the Order Rhizobiales. International Journal of Systematic Evolutionary Microbiology, 53, 1853-1859. http://dx.doi.org/10.1099/ijs.0.02644-0
Ivanova, E.P., Bawman, J.P., Lysako, A.M., Gorshkova, N.M., Kuznetsova, T.A., Kalmovskaya, N.I., Sherchemko, L.S. and Mikhailow, V.V. (2005) Erythobacter vulgaris. A Novel Organism Isolated from the Marine Invertebrates. Systematic Applied Microbiology, 28, 123-130. http://dx.doi.org/10.1016/j.syapm.2004.11.001
Bretta, S.I., Christen, R. and Hope, M.G. (2004) Belliella baltica gen. nov. sp. nov., a Novel Marine Bacteria of the Cytophage-Flavobacterium-Bacteriodes Isolated from Surface Water of the Central Baltic Sea. International Journal of Systematic and Evolutionary Microbiology, 54, 2335-2341.
Pinhassi, J., Wisding, A., Binnerup, S.J., Zweifel, U.L., Ricman, B.Y. and Hagstrom, A. (2003) Spatial Variability in Bacterioplankton Community Composition at the Skagerrak-Kattegat Front. Marine Ecology Progress Series, 255, 1-13. http://dx.doi.org/10.3354/meps255001
Essien, J.P., Benson, N.U. and Antai, P.S. (2008) Application of Correlation Analysis in Assessment of Relationships between Mineral Hydrocarbon Levels and Hydrocarbonoclastic Bacteria Count in Tropical Mangrove Estuary Sediments. Scientific Research and Essays, 3, 94-101.
Bertrand, J.C., Al-Mallah, M., Acquavva, M. and Mille, G. (1990) Biodegradation of Hydrocarbons by an Extremely Halophilic Archaebacterium. Letters in Applied Microbiology, 11, 260-263. http://dx.doi.org/10.1111/j.1472-765X.1990.tb00176.x
Bruns, A. and Berthe-Corti, L. (1999) Fundibacter jadensis gen. nov. sp. nov., a New Slightly Halophilic Bacterium Isolated from Intertidal Sediment. International Journal of Systematic Bacteriology, 49, 441-448.
Gauthier, M.J., Lafay, B., Christen, R., Fernandez, L., Acquaviva, M., Bonin, P. and Bertrand, J.C. (1992) Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a New Extremely Halotolerant Hydrocarbon-Degrading Marine Bacteria. International Journal of Systematic Bacteriology, 42, 568-576.
Mnif, S., Chamka, M. and Sayadi, S. (2009) Isolation and Characterization of Halomonas sp. Strain C255100, a Hydrocarbon Degrading Bacterium under Hypersaline Condition. Journal of Applied Microbiology, 107, 785-794.
Sei, A. and Fatherpure, B.Z. (2009) Biodegradation of BTEX at High Salinity by an Enrichment Culture from Hypersaline Sediments of Rozel Point at Great Salt Lake. Journal of Applied Microbiology, 107, 2001-2008.
Cheesebrough, M. (2002) District Laboratory Practice in Tropical Countries 1 & 2. Cambridge University Press, Cambridge, 614-661.
American Public Health Association (1998) Standard Methods for the Examination of Water and Waste Water. 20th Edition, APHA-AWWA-WPCF, Washington DC.
Kedzia, W. (1990) Microbiological Diagnostics in Medicine. PZWL, Warszawa, 367 p.
Smibert, R.M. and Kreg, N.R. (1994) Phenotypic Characterization. In: Gerhard, P., Murray, R.G., Wood, W.A. and Krieg, N.R., Eds., Methods for General and Molecular Bacteriology, American Society of Microbiology, Washington DC, 611-654.
Cabaj, A., Palinska, K., Kosakowska, A. and Kurlenda, J. (2006) Heterotrophic Bacteria from Brackish Water of Southern Baltic Sea: Biochemical and Molecular Identification and Characterization. Oceanologia, 48, 525-543.
Moore, D.M. and Dowhan, D. (2002) Current Protocols in Molecular Biology. John Wiley and Sons Inc., Hoboken.
Luo, J., Liang, H., Yan, L., Ma, J., Yang, Y.L. and Li, G.B. (2013) Microbial Community Structures in a Closed Raw Water Distribution System Biofilm as Revealed by 454-Pyrosequencing Analysis and the Effect of Microbial Biofilm Communities on Raw Water Quality. Bioresource Technology, 148, 189-195. http://dx.doi.org/10.1016/j.biortech.2013.08.109
Boivin-Johns, V., Bianchi, A., Ruimy, R., Garcin, J., Daumas, S. and Christen, R. (1995) Comparison of Phenotypical and Molecular Methods for Identification of Bacterial Stands Isolated from a Deep Subsurface Environment. Applied and Environmental Microbiology, 61, 3400-3406.
Zobell, C.E. (1946) Marine Microbiology. A Monogram on Hydrobacteriology. Chronica Botanica Co., Waltham, 240 p.
Hagstrom, A., Pinhassi, J. and Zoseifel, U.L. (2000) Biogeographically Diversity among Marine Bacterioplankton Species. Aquatic Microbial Ecology, 21, 231-244. http://dx.doi.org/10.3354/ame021231
Pinhassi, J., Zwerfel, U.L. and Hagstrom, A. (1997) Dominant Marine Bacterioplankton Species Found among Colony-Forming Bacteria. Applied and Environmental Microbiology, 63, 3359-3366.