The purpose of our study was to determine the class I and II HLA polymorphism in an Ivorian population and to evaluate whether alleles are associated to resistance or susceptibility to HIV-1 infection. The study took place from February 2021 to November 2023 at the National Blood Bank of Ivory Coast Abidjan for blood collection and the Immunogenetics laboratory of EFS in Marseille for HLA typing. We used 79 blood samples from heterosexual couples divided into 3 groups: serodiscordant couples, HIV-1 positive couples and HIV-1 negative couples. HLA phenotyping was performed by next-generation sequencing on the Miseq ® platform and allelic frequencies were calculated. Comparison of frequencies between patients and controls was made with Fisher’s exact test. We detected a total of 140 alleles (73 Class I HLA and 67 Class II HLA). The most frequent were HLA-A*74 (0.1266), HLA-B*53:01 (0.1962), HLA-C*04:01 (0.2848), HLA-DRB1*07:01 (0.1202), DQA1* 01:02 (0.2848), HLA-DQB1*02:02(0.2025), DPA1*02:01 (0.3861) and HLA-DPB1*01:01 (0.4051). Allelic frequencies were not statistically different between HIV-1 positive and negative subjects. However, we observed alleles present only in subjects living with HIV-1. This study presents for the first time high-resolution typing data of class I and II HLA alleles in Ivory Coast. We found alleles present only in HIV-1 positive subjects and others only in HIV-1 negative exposed partners suggesting their role in resistance or susceptibility to HIV-1 infection.
KeywordsHLAPolymorphismHIV-1Ivory Coast
(2024) Statistiques mondiales sur le VIH. https://www.unaids.org/sites/default/files/media_asset/UNAIDS_FactSheet_fr.pdf
Trowsdale, J. (2011) The MHC, Disease and Selection. Immunology Letters , 137, 1-8. https://doi.org/10.1016/j.imlet.2011.01.002
Blackwell, J.M., Jamieson, S.E. and Burgner, D. (2009) HLA and Infectious Diseases. Clinical Microbiology Reviews , 22, 370-385. https://doi.org/10.1128/cmr.00048-08
Espinosa, E. and Chillet, P. (2010) Immunologie. Ellipses, Paris, 512 p. https://www.eyrolles.com/Sciences/Livre/immunologie-9782729860769/
Robinson, J., Barker, D.J. and Marsh, S.G.E. (2024) 25 Years of the IPD-IMGT/HLA Database. HLA , 103, e15549. https://doi.org/10.1111/tan.15549
Jeffery, K.J.M. and Bangham, C.R.M. (2000) Do Infectious Diseases Drive MHC Diversity? Microbes and Infection , 2, 1335-1341. https://doi.org/10.1016/s1286-4579(00)01287-9
Martin, M.P., Gao, X., Lee, J., Nelson, G.W., Detels, R., Goedert, J.J., et al . (2002) Epistatic Interaction between KIR3DS1 and HLA-B Delays the Progression to Aids. Nature Genetics , 31, 429-434. https://doi.org/10.1038/ng934
Carrington, M., Nelson, G.W., Martin, M.P., Kissner, T., Vlahov, D., Goedert, J.J., et al . (1999) HLA and HIV-1: Heterozygote Advantage and B* 35- Cw* 04 Disadvantage. Science , 283, 1748-1752. https://doi.org/10.1126/science.283.5408.1748
Pereyra, F., Addo, M.M., Kaufmann, D.E., Liu, Y., Miura, T., Rathod, A., et al . (2008) Genetic and Immunologic Heterogeneity among Persons Who Control HIV Infection in the Absence of Therapy. The Journal of Infectious Diseases , 197, 563-571. https://doi.org/10.1086/526786
Ferre, A.L., Hunt, P.W., McConnell, D.H., Morris, M.M., Garcia, J.C., Pollard, R.B., et al . (2010) HIV Controllers with HLA-DRB1*13 and HLA-DQB1*06 Alleles Have Strong, Polyfunctional Mucosal Cd4 + T-Cell Responses. Journal of Virology , 84, 11020-11029. https://doi.org/10.1128/jvi.00980-10
Lambotte, O. (2012) Les patients HIV controllers. Médecine / Sciences , 28, 172-178. https://doi.org/10.1051/medsci/2012282015
Li, L.Z., Liu, Y., Miroslaw, K. and Gorny, C. (2020) Association of Diverses Genotypes and Phenotypes of Immunes Cells and Immunoglobulines with the Course of HIV-1 Infection. Frontiers in Immunology , 9, Article 2735. https://doi.org/10.3389/fimmu.2018.02735
Naruto, T., Gatanaga, H., Nelson, G., Sakai, K., Carrington, M., Oka, S., et al . (2012) HLA Class I-Mediated Control of HIV-1 in the Japanese Population, in Which the Protective HLA-B*57 and HLA-B*27 Alleles Are Absent. Journal of Virology , 86, 10870-10872. https://doi.org/10.1128/jvi.00689-12
Mori, M., Wichukchinda, N., Miyahara, R., Rojanawiwat, A., Pathipvanich, P., Maekawa, T., et al . (2014) HLA-B*35: 05 Is a Protective Allele with a Unique structure among HIV-1 CRF01_AE-Infected Thais, in Whom the B*57 Frequency Is Low. AIDS , 28, 959-967. https://doi.org/10.1097/qad.0000000000000206
Fellay, J., Ge, D., Shianna, K.V., Colombo, S., Ledergerber, B., Cirulli, E.T., et al . (2009) Common Genetic Variation and the Control of HIV-1 in Humans. PLOS Genetics , 5, e1000791. https://doi.org/10.1371/journal.pgen.1000791
[authors] (2010) The Major Genetic Determinants of HIV-1 Control Affect HLA Class I Peptide Presentation. Science , 330, 1551-1557. https://doi.org/10.1126/science.1195271
Carlson, J.M., Listgarten, J., Pfeifer, N., Tan, V., Kadie, C., Walker, B.D., et al . (2012) Widespread Impact of HLA Restriction on Immune Control and Escape Pathways of HIV-1. Journal of Virology , 86, 5230-5243. https://doi.org/10.1128/jvi.06728-11
Leslie, A., Matthews, P.C., Listgarten, J., Carlson, J.M., Kadie, C., Ndung’u, T., et al . (2010) Additive Contribution of HLA Class I Alleles in the Immune Control of HIV-1 Infection. Journal of Virology , 84, 9879-9888. https://doi.org/10.1128/jvi.00320-10
Kiepiela, P., Leslie, A.J., Honeyborne, I., Ramduth, D., Thobakgale, C., Chetty, S., et al . (2004) Dominant Influence of HLA-B in Mediating the Potential Co-Evolution of HIV and Hla. Nature , 432, 769-775. https://doi.org/10.1038/nature03113
Gao, X., Nelson, G.W., Karacki, P., Martin, M.P., Phair, J., Kaslow, R., et al . (2001) Effect of a Single Amino Acid Change in MHC Class I Molecules on the Rate of Progression to Aids. New England Journal of Medicine , 344, 1668-1675. https://doi.org/10.1056/nejm200105313442203
Tang, J., Malhotra, R., Song, W., Brill, I., Hu, L., Farmer, P.K., et al . (2010) Human Leukocyte Antigens and HIV Type 1 Viral Load in Early and Chronic Infection: Predominance of Evolving Relationships. PLOS ONE , 5, e9629. https://doi.org/10.1371/journal.pone.0009629
Ellis, J.M., Hoyer, R.J., Costello, C.N., Mshana, R.N., Quakyi, I.A., Mshana, M.N., et al . (2001) HLA-B Allele Frequencies in Côte D’ivoire Defined by Direct DNA Sequencing: Identification of HLA-B*1405, B*4410, and B*5302. Tissue Antigens , 57, 339-343. https://doi.org/10.1034/j.1399-0039.2001.057004339.x
Jennes, W., Verheyden, S., Demanet, C., Adjé-Touré, C.A., Vuylsteke, B., Nkengasong, J.N., et al . (2006) Cutting Edge: Resistance to HIV-1 Infection among African Female Sex Workers Is Associated with Inhibitory KIR in the Absence of Their HLA Ligands. The Journal of Immunology , 177, 6588-6592. https://doi.org/10.4049/jimmunol.177.10.6588
Masciotra, S., Luo, W., Youngpairoj, A.S., Kennedy, M.S., Wells, S., Ambrose, K., et al . (2013) Performance of the Alere Determine™ HIV-1/2 Ag/ab Combo Rapid Test with Specimens from HIV-1 Seroconverters from the US and HIV-2 Infected Individuals from Ivory Coast. Journal of Clinical Virology , 58, e54-e58. https://doi.org/10.1016/j.jcv.2013.07.002
Loukou, Y.G., Cabran, M.A., Yessé, Z.N., Adouko, B.M.O., Lathro, S.J. and Agbessi-Kouassi, K.B.T. (2013) Performance of Rapid Tests and Algorithms for HIV Screening in Abidjan, Ivory Coast. Journal of the International Association of Providers of AIDS Care ( JIAPAC ), 13, 35-39. https://doi.org/10.1177/2325957413488168
(2016) Document de politique, normes et procédures des services de dépistage du VIH en Côte d’Ivoire. https://www.pnlsci.com/wp-content/uploads/2022/02/directives-2016-depistage-vih.pdf
Ahadri, N. (2012) Le transport des échantillons biologiques. Option / Bio , 23, 19-22. https://doi.org/10.1016/s0992-5945(12)70991-1
Alizadeh, M., Picard, C., Frassati, C., Walencik, A., Gauthier, A.C., Bennasar, F., et al . (2020) A New Set of Reagents and Related Software Used for NGS Based Classical and Non-Classical HLA Typing Showing Evidence for a Greater HLA Haplotype Diversity. Human Immunology , 81, 202-205. https://doi.org/10.1016/j.humimm.2020.02.003
(2024) IPD-IMGT/HLA Data Base. https://www.ebi.ac.uk/ipd/imgt/hla/alleles/
Angulo, J.M.C., Cuesta, T.A.C., Menezes, E.P., Pedroso, C. and Brites, C. (2019) A Systematic Review on the Influence of HLA-B Polymorphisms on HIV-1 Mother-to-Child-Transmission. The Brazilian Journal of Infectious Diseases , 23, 53-59. https://doi.org/10.1016/j.bjid.2018.12.002
Nii-Trebi, N.I., Matsuoka, S., Kawana-Tachikawa, A., Bonney, E.Y., Abana, C.Z., Ofori, S.B., et al . (2022) Super High-Resolution Single-Molecule Sequence-Based Typing of HLA Class I Alleles in HIV-1 Infected Individuals in Ghana. PLOS ONE , 17, e0269390. https://doi.org/10.1371/journal.pone.0269390
Sanchez-Mazas, A., Černý, V., Di, D., Buhler, S., Podgorná, E., Chevallier, E., et al . (2017) The HLA-B Landscape of Africa: Signatures of Pathogen-Driven Selection and Molecular Identification of Candidate Alleles to Malaria Protection. Molecular Ecology , 26, 6238-6252. https://doi.org/10.1111/mec.14366
Cargou, M., Ralazamahaleo, M., Blouin, L., Guidicelli, G. and Visentin, J. (2018) Characterization of the Novel HLA - DPA1*02: 12 Allele by Sequencing-Based Typing. HLA , 93, 61-62. https://doi.org/10.1111/tan.13418
Sherrill, J.B., Cereb, N. and Ho, C. (2019) Identification of a Novel Allele, HLA - DPB 1*417: 01: 02, in an African American Deceased Donor. HLA , 94, 88-88. https://doi.org/10.1111/tan.13526
(2024) La base de données Allele Frequency Net [Rechercher les fréquences des allèles HLA]. https://www.allelefrequencies.net/hla6006a.asp
Hill, A.V., Allsopp, C.E., Kwiatkowski, D., Taylor, T.E., Yates, S.N., Anstey, N.M., et al . (1992) Extensive Genetic Diversity in the HLA Class II Region of Africans, with a Focally Predominant Allele, DRB1*1304. Proceedings of the National Academy of Sciences of the United States of America , 89, 2277-2281. https://doi.org/10.1073/pnas.89.6.2277
Peterson, T.A., Bielawny, T., Lacap, P., Hardie, R., Daniuk, C., Mendoza, L., et al . (2014) Diversity and Frequencies of HLA Class I and Class II Genes of an East African Population. Open Journal of Genetics , 4, 99-124. https://doi.org/10.4236/ojgen.2014.42013
Tshabalala, M., Mellet, J., Vather, K., Nelson, D., Mohamed, F., Christoffels, A., et al . (2022) High Resolution HLA ∼A, ∼B, ∼C, ∼DRB1, ∼DQA1, and ∼DQB1 Diversity in South African Populations. Frontiers in Genetics , 13, Article 711944. https://doi.org/10.3389/fgene.2022.711944
Cao, K., Moormann, A.M., Lyke, K.E., Masaberg, C., Sumba, O.P., Doumbo, O.K., et al . (2004) Differentiation between African Populations Is Evidenced by the Diversity of Alleles and Haplotypes of HLA Class I Loci. Tissue Antigens , 63, 293-325. https://doi.org/10.1111/j.0001-2815.2004.00192.x
Le, W., Shi, J., Zhang, T., Liu, L., Qin, H., Liang, S., et al . (2016) HLA-DRB1*15: 01 and HLA-DRB3*02: 02 in PLA2R-Related Membranous Nephropathy. Journal of the American Society of Nephrology , 28, 1642-1650. https://doi.org/10.1681/asn.2016060644
Goulder, P.J.R. and Watkins, D.I. (2008) Impact of MHC Class I Diversity on Immune Control of Immunodeficiency Virus Replication. Nature Reviews Immunology , 8, 619-630. https://doi.org/10.1038/nri2357
Luo, M., Daniuk, C.A., Diallo, T.O., Capina, R.E., Kimani, J., Wachihi, C., et al . (2012) For Protection from HIV-1 Infection, More Might Not Be Better: A Systematic Analysis of HIV Gag Epitopes of Two Alleles Associated with Different Outcomes of HIV-1 Infection. Journal of Virology , 86, 1166-1180. https://doi.org/10.1128/jvi.05721-11
Lobos, C.A., Downing, J., D’Orsogna, L.J., Chatzileontiadou, D.S.M. and Gras, S. (2022) Protective HLA-B57: T Cell and Natural Killer Cell Recognition in HIV Infection. Biochemical Society Transactions , 50, 1329-1339. https://doi.org/10.1042/bst20220244
Trachtenberg, E.A. and Erlich, H.A. (2001) A Review of the Role of the Human Leukocyte Antigen (HLA) System as a Host Immunogenetic Factor Influencing HIV Transmission and Progression to AIDS. HIV Molecular Immunology , 1, 43-60. https://hfv.lanl.gov/content/immunology/pdf/2001/1/4-trachtenburg.pdf
Julg, B., Moodley, E.S., Qi, Y., Ramduth, D., Reddy, S., Mncube, Z., et al . (2011) Possession of HLA Class II DRB1*1303 Associates with Reduced Viral Loads in Chronic HIV-1 Clade C and B Infection. The Journal of Infectious Diseases , 203, 803-809. https://doi.org/10.1093/infdis/jiq122
Sidney, J., Peters, B., Frahm, N., Brander, C. and Sette, A. (2008) HLA Class I Supertypes: A Revised and Updated Classification. BMC Immunology , 9, Article No. 1. https://doi.org/10.1186/1471-2172-9-1
Ou, G., Liu, X. and Jiang, Y. (2021) HLA-DPB1 Alleles in Hepatitis B Vaccine Response. Medicine , 100, e24904. https://doi.org/10.1097/md.0000000000024904
Bettencourt, A., Carvalho, C., Leal, B., Brás, S., Lopes, D., Martins da Silva, A., et al . (2015) The Protective Role of HLA-DRB113 in Autoimmune Diseases. Journal of Immunology Research , 2015, Article ID: 948723. https://doi.org/10.1155/2015/948723