No Dichotomy in Double Counting of CD4 + T Cell Values on PIMA™® and BD FACSPresto™® in Benin — Oak Academic Publishing
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No Dichotomy in Double Counting of CD4 + T Cell Values on PIMA™® and BD FACSPresto™® in Benin
Reference Laboratory of Health Program Fighting Against AIDS in Benin (LR/PSLS), Health Ministry of Benin, Cotonou, Benin
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Health Ministry, Cotonou, Benin
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Institute for Health Research, Epidemiological Surveillance and Training (IRESSEF), Dakar, Senegal
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Laboratory of Cell Biology, Physiology and Immunology, Department of Biochemistry and Cellular Biology, Faculty of Sciences and Technology (FAST) and Institute of Applied Biomedical Sciences (ISBA), University of Abomey-Calavi, Cotonou, Benin
1 Reference Laboratory of Health Program Fighting Against AIDS in Benin (LR/PSLS), Health Ministry of Benin, Cotonou, Benin
2 Health Ministry, Cotonou, Benin
3 Institute for Health Research, Epidemiological Surveillance and Training (IRESSEF), Dakar, Senegal
4 Laboratory of Cell Biology, Physiology and Immunology, Department of Biochemistry and Cellular Biology, Faculty of Sciences and Technology (FAST) and Institute of Applied Biomedical Sciences (ISBA), University of Abomey-Calavi, Cotonou, Benin
The survival of PLHIV-1 by the count of CD4 + T lymphocytes remains essential to predict the treatment of opportunistic infections. The aim of the study was to compare the performance of PIMA™® and FACSPresto™® to the conventional equipment of FACSCount™® through CD4 + T cell counting. CD4 + T lymphocytes were tested both on PIMA™® and BD FACSPresto™®. Values were compared to those obtained from FACSCount™®, using Passing Bablok, Bland-Altman and Pollock diagrams. PIMA™® and FACSPresto™® present a good correlation coefficient with the Passing Bablok diagram (y = −0.5982 + 0.9940x; ρ c = 0.9969) and (y = 7.2913 + 0.9974x; ρ c = 0.9972) respectively. Bland-Altman and Pollock plots show mean biases of 3.7 cells/μL (LOA ranging from −62.7 to 60.1) and 0.5 cells/μL (LOA ranging from −10.7 to 11.6) for PIMA™® and −1.5 cells/μL (LOA ranging from −45.7 to 42.7) and −1.9 cells/μL (LOA ranging from −23.4 to 19.6) for FACSPresto™®. Sensitivity and specificity of PIMA™® and FACSPresto™® vary respectively between 98 to 99% and 96 to 98% at threshold of 350 cells/μL and 98 to 100% at threshold of 500 cells/μL. Both technologies highlight their ability to be used as an alternative to the reference technique.
KeywordsBD FACSCount™®FACSPresto™®PIMA™®PLHIV-1CD4 + T cellBenin
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