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Identification of a T-cell epitope in the <i>Staphylococcus aureus</i> Panton-Valentine LukS-PV component
Center for Infectious Disease, University of Texas School of Public Health, Houston, USA
Department of Pathology and Laboratory Medicine, University Texas-Houston Medical School, Houston, USA
Institute of Biosciences and Technology, Texas A&M University Health Science Center, Houston, USA;
- 1 Center for Infectious Disease, University of Texas School of Public Health, Houston, USA
- 2 Department of Pathology and Laboratory Medicine, University Texas-Houston Medical School, Houston, USA
- 3 Institute of Biosciences and Technology, Texas A&M University Health Science Center, Houston, USA;
Open Journal of Immunology·Volume 02 (2012)·Pages 111–115·Published 21 September 2012·DOI10.4236/oji.2012.23013
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Abstract
We previously observed the elicitation of a significant delayed-type hypersensitivity response to the Panton-Valentine leukocidin (PVL) LukS-PV subunit following subcutaneous immunization (Brown et al ., Clinical Microbiology and Infection , 2009, 156: 156-164). A LukS-PV-specific cell line (L S T) was screened for proliferative responses against a panel of 25 amino acid-long peptides spanning the length of LukS-PV (amino acids 29-312). This analysis demonstrated that stimulation of L S T with LukS-PV resulted in significant proliferative responses and adoptive transfer of L S T into na?ve mice conferred a LukS-PV-specific DTH response following challenge. Challenge of mice adoptively transferred with L S T with peptides 7 (149-173), 8 (169-193) and 14 (289-312) also elicited a measurable DTH response suggesting that these peptides contained T cell epitopes.
KeywordsStaphylococcusCell Mediated ImmunityPanton-Valentine LeukocidinT Cells
- Klevens, R.M., Morrison, M.A., Nadle, J., Petit, S., Gershman, K., Ray, S., Harrison, L.H., Lynfield, R., Dumyati, G., Townes, J.M., Craig, A.S., Zell, E.R., Fosheim, G.E., McDougal, L.K., Carey, R.B. and Fridkin, S.K. (2007) Invasive methicillin-resistant Staphylococcus aureus infections in the united states. Journal of the American Medical Association, 298, 1763-1771. doi:10.1001/jama.298.15.1763
- Lowy F.D. (1998) Staphylococcus aureus infections. New England Journal of Medicine, 339, 520-532. doi:10.1056/NEJM199808203390806
- Brown, E.L., Dumitrescu, O., Thomas, D., Badiou, C., Koers, E.M., Choudhury, P., Vazquez, V., Etienne, J., Lina, G., Vandenesch, F. and Bowden, M.G. (2009) The panton-valentine leukocidin vaccine protects mice against lung and skin infections caused by staphylococcus aureus USA300. Clinical Microbiology and Infection, 156, 156-164. doi:10.1111/j.1469-0691.2008.02648.x
- Voyich, J.M., Otto, M., Mathema, B., Braughton, K.R., Whitney, A.R., Welty, D., Long, R.D., Dorward, D.W., Gardner, D.J., Lina, G., Kreiswirth, B.N., DeLeo, F.R. (2006) Is panton-valentine leukocidin the major virulence determinant in community-associated methicillin-resis-tant staphylococcus aureus disease? Journal of Infectioius Diseases, 194, 1761-1770. doi:10.1086/509506
- Pride M.W., Brown E.L., Stephens L.C., Killion J.J., Norris S.J. and Kripke M.L. (1998) Specific th1 cell lines that confer protective immunity against experimental borrelia burgdorferi infection in mice. Journal of Leukocyte Biology, 63, 542-549.
- Proctor R.A. (2012) Challenges for a universal Staphylococcus aureus vaccine. Clinical Infectious Diseases, 54, 1179-1186. doi:10.1093/cid/cis033
- Gomez M.I., Sordelli D.O., Buzzola F.R. and Garcia V.E. (2002) Induction of cell-mediated immunity to staphylococcus aureus in the mouse mammary gland by local immunization with a live attenuated mutant. Infection and Immunity, 70, 4254-4260. doi:10.1128/IAI.70.8.4254-4260.2002
- Guillen C., McInnes, I.B., Vaughan, D.M., Kommajosyula, S., Van Berkel, P.H., Leung, B.P., Aguila, A. and Brock, J.H. (2002) Enhanced Th1 response to Staphylococcus aureus infection in human lactoferrin-transgenic mice. Journal of Immunology, 168, 3950-3957.
- Lin, L., Ibrahim, A.S., Xu, X., Farber, J.M., Avanesian, V., Baquir, B., Fu, Y., French, S.W., Edwards, J.E. and Spellberg, B. (2009) Th1-th17 cells mediate protective adaptive immunity against staphylococcus aureus and candida albicans infection in mice. PLoS Pathogens, 5, e1000703. doi:10.1371/journal.ppat.1000703