Mitochondrial DNA Markers for PCR-Based Phylogenetic Analysis of Ark Shells — Oak Academic Publishing
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Mitochondrial DNA Markers for PCR-Based Phylogenetic Analysis of Ark Shells
United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan; Coastal Lagoon Research Center, Shimane University, Matsue, Japan
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United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan; Coastal Lagoon Research Center, Shimane University, Matsue, Japan
1 United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan; Coastal Lagoon Research Center, Shimane University, Matsue, Japan
2 United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan; Coastal Lagoon Research Center, Shimane University, Matsue, Japan
Arcidae species are commercially important bivalves in Japan and are commonly referred to as bloody ark due to their red blood. They have thick shells with distinct radiating ribs, and the numbers of these ribs are important morphological features for species discrimination. However, some Arcidae species are morphologically indistinguishable, with a simi lar number of the ribs in adults and deficient rib formation, particularly among juveniles. Thus, we developed a reliable molecular marker to genetically discriminate between 7 Arcidae species belonging to Scapharca , Anadara , and Tegil larca based on species-specific polymorphic segments of mitochondrial DNA. PCR amplification of partial COI, 16S rRNA, 12S rRNA, and Cyt b genes was performed on 7 species using 8 primer sets. Only the set of Scapharca -specific forward primer and universal reverse primer for the partial COI gene successfully yielded single PCR products from all 7 species examined. Thus, nucleotide sequences of 481 bp portion of these PCR products were determined, and the de grees of nucleotide substitutions ranged from 0.4% between S . broughtonii and T . granosa to 20.2% between S . satowi and A . antiquata . In addition, a phylogenetic tree showed significant differences between 7 species, with higher boot strap support than 69 .
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