Fecal coliform bacteria such as <i>Escher</i><i>ichia</i><i> </i><i>coli</i> (<i>E.</i><i> </i><i>coli</i>) are one of the main sources of groundwater pollution. An assessment of the transport and Persistence of <i>E.</i><i> </i><i>c</i>oli in poultry litter amended Decatur silty Clay soil and Hartsells Sandy soil was conducted using soil columns and simulated groundwater leaching. Enumeration of initial <i>E.</i><i> </i><i>coli</i> was determined to range from 2.851 × 10<sup>3</sup> to 3.044 × 10<sup>3</sup> CFU per gram of soil. These results have been used in a batch study to determine the persistence rate of <i>E.</i><i> </i><i>coli</i> in Decatur silty Clay soil and Hartsells Sandy soil. Results prove that <i>E.</i><i> </i><i>coli</i> survival growth rate increases for clay soil later than and at a higher rate than sandy soil. The column study has determined that <i>E.</i><i> </i><i>coli</i> was transported at a rate of 3.7 × 10<sup>6</sup><sup> </sup>CFU for Decatur silty loam and 6.3 × 10<sup>6</sup><sup> </sup>CFU for Hartsells sandy per gram of soil. Further, linear regression analysis predictions show higher porosity and soil moisture content affect transport, and Hartsells sandy soil has higher transport of <i>E.</i><i> </i><i>coli</i><i> </i>due to its higher porosity and lower volumetric water content.
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