Background: Traditional medicine has employed Azadirachta indica to treat a variety of ailments. However, there is little information on the use of this plant in Zambia. Objective: To evaluate the phytochemicals and antibacterial activity of Azadirachta indica leaf extracts against Escherichia coli. Materials and Methods: This was a laboratory-based experimental study in which ethanol and water were used for extraction by maceration. Phytochemical analysis was then done on the leaf extract. Using the disc diffusion method, varying concentrations of A. indica aqueous and ethanolic extracts were used to test the antibacterial activity of A. indica against Escherichia co li . The sensitivity of the tested microorganisms to aqueous and ethanolic leaf extracts was shown by zones of inhibition after incubation. Results: The phytochemical screening of the A. indica leaves revealed the presence of phenolics and tannins in both the ethanol and aqueous extract. Saponins, flavonoids and alkaloids were only present in the aqueous extract. While steroids were only present in the ethanol extract. The antibacterial activity of A. indica leaves extract was determined by zones of inhibition which showed that both aqueous and ethanol extracts had activity against E. coli . The Minimum Inhibitory Concentration was determined at 10 mg/mL for the aqueous extract and 20 mg/mL for the ethanol extract. The zones of inhibition increased with concentration. Conclusion: The extracts of A. indica displayed antibacterial activity against E. coli in a dose-dependent manner. Comparatively, the aqueous extract produced better antibacterial properties against E. coli than the ethanolic extract.
Nachega, J., Seydi, M. and Zumla, A. (2020) The Late Arrival of Coronavirus Disease 2019 (COVID-19) in Africa: Mitigating Pan-Continental Spread. Clinical Infectious Diseases, 71, 875-878. https://doi.org/10.1093/cid/ciaa353
Kindhauser, M.K. and WHO (2003) Communicable Diseases 2002: Global Defence against the Infectious Disease Threat. World Health Organization.
Madappa, T. (2012) Escherichia coli Infections. Emedicine Medscape. http://emedicine.medscape.com/article/217485-overview#a0101
Holmes, A.H., Moore, L.S., Sundsfjord, A., Steinbakk, M., Regmi, S., Karkey, A., et al. (2016) Understanding the Mechanisms and Drivers of Antimicrobial Resistance. The Lancet, 387, 176-187. https://doi.org/10.1016/S0140-6736(15)00473-0
Masaiti, G.C., Malambo, C., Hikaambo, C., Banda, M., Matafwali, S.K., Mufwambi, W., et al. (2019) Antibacterial Properties of Ficus sycomorus Bark Extract against Staphylococcus aureus and Escherichia coli. International Journal of Biomedical Investigation, 2, 1-7. https://doi.org/10.31531/2581-4745.1000121
Allocati, N., Masulli, M., Alexeyev, M.F. and Di Ilio, C. (2013) Escherichia coli in Europe: An Overview. International Journal of Environmental Research and Public Health, 10, 6235-6254. https://doi.org/10.3390/ijerph10126235
Apenteng, J.A., Osei-Asare, C., Oppong, E.E., Amihere, I. and Hafiz, M.Y. (2017) Antibiotic Sensitivity Patterns of Microbial Isolates from Fish Ponds: A Study in the Greater Accra Region of Ghana. African Journal of Pharmacy and Pharmacology, 11, 314-320. https://doi.org/10.5897/AJPP2017.4789
Kibret, M. and Abera, B. (2011) Antimicrobial Susceptibility Patterns of E. coli from Clinical Sources in Northeast Ethiopia. African Health Sciences, 11, 40-45. https://doi.org/10.4314/ahs.v11i3.70069
Aslam, B., Wang, W., Arshad, M.I., Khurshid, M., Muzammil, S., Rasool, M.H., et al. (2018) Antibiotic Resistance: A Rundown of a Global Crisis. Infection and Drug Resistance, 11, 1645-1658. https://doi.org/10.2147/IDR.S173867
Morris, S. and Cerceo, E. (2020) Trends, Epidemiology, and Management of Multi-Drug Resistant Gram-Negative Bacterial Infections in the Hospitalized Setting. Antibiotics, 9, Article No. 196. https://doi.org/10.3390/antibiotics9040196
Mazzariol, A., Bazaj, A. and Cornaglia, G. (2017) Multi-Drug-Resistant Gram-Negative Bacteria Causing Urinary Tract Infections: A Review. Journal of Chemotherapy, 29, 2-9. https://doi.org/10.1080/1120009X.2017.1380395
Chinsembu, K. (2016) Ethnobotanical Study of Medicinal Flora Utilised by Traditional Healers in the Management of Sexually Transmitted Infections in Sesheke District, Western Province, Zambia. Revista Brasileira de Farmacognosia, 26, 268-274. https://doi.org/10.1016/j.bjp.2015.07.030
Maleki, L., Sadeghian-Rizi, T., Ghannadian, M., Sanati, M.H., Shafizadegan, S. and Sadeghi-Aliabadi, H. (2017) Antibacterial Activity of Azadirachta indica Leaf Extracts against Some Pathogenic Standards and Clinical Bacterial Isolates. Avicenna Journal of Clinical Microbiology and Infection, 5, Article ID: 12987. https://doi.org/10.5812/ajcmi.12987
Pandey, G., Verma, K. and Singh, M. (2014) Evaluation of Phytochemical, Antibacterial and Free Radical Scavenging Properties of Azadirachta indica (Neem) Leaves. International Journal of Pharmacy and Pharmaceutical Sciences, 6, 444-447.
Hashmat, I., Azad, H. and Ahmed, A. (2012) Neem (Azadirachta indica A. Juss)—A Nature’s Drugstore: An Overview. International Research Journal of Biological Sciences, 1, 76-79.
Evans, W.C. (2002) Trease and Evans’ Pharmacognosy. WB Saunders, New York, 553-557.
Ogbeba, J., Iruolaje, F. and Dogo, B. (2017) Antimicrobial Efficacy of Guiera senegalensis and Prosopis africana Leave Extract on Some Bacterial Pathogens. European Journal of Biology and Medical Science Research, 5, 27-36.
Beyene, G. and Tsegaye, W. (2011) Bacterial Uropathogens in Urinary Tract Infection and Antibiotic Susceptibility Pattern in Jimma University Specialized Hospital, Southwest Ethiopia. Ethiopian Journal of Health Sciences, 21, 141-146. https://doi.org/10.4314/ejhs.v21i2.69055
Idelevich, E.A., Storck, L.M., Sparbier, K., Drews, O., Kostrzewa, M. and Becker, K. (2018) Rapid Direct Susceptibility Testing from Positive Blood Cultures by the Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Direct-on-Target Microdroplet Growth Assay. Journal of Clinical Microbiology, 56, e00913-e00918.
Brown-Elliott, B.A., Nash, K.A. and Wallace, R.J. (2012) Antimicrobial Susceptibility Testing, Drug Resistance Mechanisms, and Therapy of Infections with Nontuberculous mycobacteria. Clinical Microbiology Reviews, 25, 545-582.
Abalkhil, T.A., Alharbi, S.A., Salmen, S.H. and Wainwright, M. (2017) Bactericidal Activity of Biosynthesized Silver Nanoparticles against Human Pathogenic Bacteria. Biotechnology & Biotechnological Equipment, 31, 411-417. https://doi.org/10.1080/13102818.2016.1267594
Bashiti, T.A., Elmanama, A.A. and Masad, A.A. (2011) The Antibacterial and Synergistic Effects of Some Palestinian Plant Extracts on Escherichia coli and Staphylococcus aureus. Functional Plant Science and Biotechnology, 5, 57-62.
Margaret, P., Duo, L., Tengfei, L. and Andrew, B. (2020) Berberine verses Sorafenib for the Treatment of Hepatocellular carcinoma. International Journal of Biomedical Investigation, 3, 1-9. https://doi.org/10.31531/2581-4745.1000125
Kubmarawa, D., Khan, M., Punah, A. and Hassan, M. (2008) Phytochemical Screening and Antimicrobial Efficacy of Extracts from Khaya senegalensis against Human Pathogenic Bacteria. African Journal of Biotechnology, 7, 4563-4566.
Sahrawat, A., Sharma, J., Rahul, S., Tiwari, S., Joshi, M. and Pundhir, A. (2018) Phytochemical Analysis and Antibacterial Properties of Azadirachta indica (Neem) Leaves Extract against E. coli. Journal of Pharmacognosy and Phytochemistry, 7, 1368-1371.
Gupta, A.K., Ahirwar, N.K., Shinde, N., Choudhary, M., Rajput, Y.S. and Singh, A. (2013) Phytochemical Screening and Antimicrobial Assessment of Leaves of Adhatoda vasica, Azadirachta indica and Datura stramonium. Pharmaceutical and Biosciences Journal, 1, 42-47. https://doi.org/10.20510/ukjpb/1/i1/91118
Susmitha, S., Vidyamol, K., Ranganayaki, P. and Vijayaragavan, R. (2013) Phytochemical Extraction and Antimicrobial Properties of Azadirachta indica (Neem). Global Journal of Pharmacology, 7, 316-320.
Nigussie, D., Davey, G., Legesse, B.A., Fekadu, A. and Makonnen, E. (2021) Antibacterial Activity of Methanol Extracts of the Leaves of Three Medicinal Plants against Selected Bacteria Isolated from Wounds of Lymphoedema Patients. BMC Complementary Medicine and Therapies, 21, Article No. 2. https://doi.org/10.1186/s12906-020-03183-0
Shu’aibu, I., Hamman, J., Goje, L., Mu’inat, A., Jauro, H. and Kabiru, M. (2015) Phytochemical Analysis and Evaluation of Bacteriostatic Effect of Neem (Azadirachta indica) Leaves on Some Clinical Bacterial Isolates. Journal of Harmonized Research in Applied Sciences, 3, 152-157.