Anti-Inflammatory and Antioxidant Properties of the Aqueous Extracts of the Leaves of <i>Opilia amentacea</i> (Opiliaceae) — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Anti-Inflammatory and Antioxidant Properties of the Aqueous Extracts of the Leaves of <i>Opilia amentacea</i> (Opiliaceae)
Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
,
Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
,
Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
,
Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
,
Ecole Doctorale Sciences et Technologie, Laboratoire de Chimie Organique et Physique Appliquée (LCOPA), Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso
,
Ecole Doctorale Sciences et Techniques, Laboratoire de Biochimie et Chimie Appliquées (LABIOCA), Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso
,
Ecole Doctorale Sciences de la Santé, Laboratoire de Développement du Médicament (LADME), Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso
,
Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
,
Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
,
Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
,
Ecole Doctorale Sciences de la Santé, Laboratoire de Développement du Médicament (LADME), Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso
,
Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
1 Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
2 Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
3 Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
4 Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
5 Ecole Doctorale Sciences et Technologie, Laboratoire de Chimie Organique et Physique Appliquée (LCOPA), Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso
6 Ecole Doctorale Sciences et Techniques, Laboratoire de Biochimie et Chimie Appliquées (LABIOCA), Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso
7 Ecole Doctorale Sciences de la Santé, Laboratoire de Développement du Médicament (LADME), Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso
8 Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
9 Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
10 Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
11 Ecole Doctorale Sciences de la Santé, Laboratoire de Développement du Médicament (LADME), Centre de Formation, de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM), Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso
Opilia amentacea (Opiliaceae) is a woody plant with multiple medicinal claimed effects. The present study aimed to assess the anti-inflammatory and antioxidant activities of the decoction and macerate extracts from the leaves of Opilia amentacea . Moreover, acute toxicity and phytochemical analysis were performed. The acute toxicity was evaluated on NMRI mice at 2000 mg/kg bw. The anti-inflammatory activity was studied using the carrageenan-induced mouse paw edema and the lipoxygenase inhibition assay. The radical scavenging (DPPH and ABTS), ferric-reducing antioxidant power (FRAP), and lipid peroxidation (LPO) assays were used to measure the antioxidant capacity of the extracts. Qualitative and quantitative methods served for identifying and quantifying the extract’s phytoconstituents. The decoction demonstrated low acute toxicity; the lethal dose was therefore estimated to be superior to 2000 mg/kg bw. The extracts significantly reduced the mouse paw’s thickness at 600 mg/kg bw. The extracts developed weak radical scavenging and lipid peroxidation inhibitory effects. However, the macerate showed a high ability (664.90 ± 0.71 mol Ascorbic Acid Equivalent/g dry extract) to reduce the ferric ions. Saponins, sterols, triterpenes, and flavonoids were qualitatively detected in the two extracts. Total phenolics (TP) and total flavonoids (TF) were found abundant in the extracts, especially the decoction (TP content (TPC) = 94.03 ± 2.66 mg GAE/g; TF content (TFC) = 35.05 ± 0.32 mg QE/g). Strong positive correlations existed between ferric-reducing capacity and TPC (r = 0.959) for the macerate, while TFC was mainly involved in the DPPH radical scavenging of the two extracts. Instead, most correlations were negative between the polyphenol compounds and the anti-inflammatory assays. The results indicate potent in vivo anti-inflammatory and in vitro antioxidant effects of the aqueous extracts from the leaves of Opilia amentacea . Further studies are needed to find the anti-inflammatory and antioxidant effects mechanism.
Musa, H.H., et al. (2022) Traditional Herbal Medicine: Overview of Research Indexed in the Scopus Database. Advances in Traditional Medicine. https://doi.org/10.1007/s13596-022-00670-2
West African Health Organisation (WAHO) (2013) West African Herbal Pharmacopoeia. https://www.wahooas.org/web-ooas/sites/default/files/publications/2185/west-african-herbal-pharmacopoeiaok.pdf
Chaitanya, M.V.N.L., et al. (2021) Traditional African Medicine. In: El-Shemy, H., Ed., Natural Medicinal Plants, IntechOpen, London, 1-9. https://doi.org/10.5772/intechopen.96576
Ezekwesili-Ofili, J.O. and Okaka A.N.A.C. (2019) Herbal Medicines in African Traditional Medicine. In: Builders, P.F., Ed., Herbal Medicine, Intechopen, London, 191-214.
Ali Elkordy, A., Haj-Ahmad, R.R., Awaad, A.S. and Zaki, R.M. (2021) An Overview on Natural Product Drug Formulations from Conventional Medicines to Nanomedicines: Past, Present and Future. Journal of Drug Delivery Science and Technology, 63, Article ID: 102459. https://doi.org/10.1016/j.jddst.2021.102459
Zhou, Y.X., Wang, D.D. and Yan, W.J. (2023) Treatment Effects of Natural Products on Inflammatory Bowel Disease in Vivo and Their Mechanisms: Based on Animal Experiments. Nutrients, 15, Article 1031. https://doi.org/10.3390/nu15041031
World Flora Online (WFO) (2023) Opilia celtidifolia Endl. ex Walp. http://www.worldfloraonline.org/taxon/wfo-0000387257#E
Muniyandi, K., et al. (2019) Effects of in vitro Simulated Gastrointestinal Digestion on the Antioxidant, α-Glucosidase and α-Amylase Inhibitory Activities of Water-Soluble Polysaccharides from Opilia amentacea Roxb Fruit. LWT, 111, 774-781. https://doi.org/10.1016/j.lwt.2019.05.079
Gronhaug, T.E., et al. (2008) Ethnopharmacological Survey of Six Medicinal Plants from Mali, West-Africa. Journal of Ethnobiology and Ethnomedicine, 4, Article No. 26. https://doi.org/10.1186/1746-4269-4-26
Youl, O., et al. (2021) Preliminary Screening of the Antimicrobial Activity of Nine Medicinal Plant Species from Burkina Faso. Journal of Medicinal Plants Research, 15, 522-530. https://doi.org/10.5897/JMPR2021.7106
Amang, A.P., et al. (2020) Hepatoprotective Effects of Aqueous Extract of Opilia celtidifolia (Opiliaceae) Leaves against Ethanol-Induced Liver Damage in Rats. Evidence-Based Complementary and Alternative Medicine, 2020, Article ID: 6297475. https://doi.org/10.1155/2020/6297475
12 Laboratoire de Recherche-Développement/Phytomédicaments et Médicaments (LR-D/PM), Institut de Recherche en Sciences de la Santé (IRSS), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso
Koudouvo, K., et al. (2011) In vitro Antiplasmodial Activity of Crude Extracts from Togolese Medicinal Plants. Asian Pacific Journal of Tropical Medicine, 4, 129-132. https://doi.org/10.1016/S1995-7645(11)60052-7
Sanon, S., et al. (2013) In Vitro Antiplasmodial and Cytotoxic Properties of Some Medicinal Plants from Western Burkina Faso. African Journal of Laboratory Medicine, 2, a81. https://doi.org/10.4102/ajlm.v2i1.81
Konaté, K., et al. (2014) Free Radicals Scavenging Capacity, Antidiabetic and Antihypertensive Activities of Flavonoid-Rich Fractions from Leaves of Trichilia emetica and Opilia amentacea in an Animal Model of Type 2 Diabetes Mellitus. Evidence-Based Complementary and Alternative Medicine, 2014, Article ID: 867075. https://doi.org/10.1155/2014/867075
Togola, A., Karabinta, K., Denou, A., Haidara, M., Sanogo, R. and Diallo, D. (2009) Effet protecteur des feuilles de Opilia celtidifolia contre l’ulcère induit par l’éthanol chez le rat. International Journal of Biological and Chemical Sciences, 8, 2416-2423. https://doi.org/10.4314/ijbcs.v8i6.5
Maiga, A., Diallo, D., Fane, S., Sanogo, R., Paulsen, B.S. and Cisse, B. (2005) A Survey of Toxic Plants on the Market in the District of Bamako, Mali: Traditional Knowledge Compared with a Literature Search of Modern Pharmacology and Toxicology. Journal of Ethnopharmacology, 96, 183-193. https://doi.org/10.1016/j.jep.2004.09.005
Inngjerdingen, K.T., et al. (2013) Pectic Polysaccharides Isolated from Malian Medicinal Plants Protect against Streptococcus pneumoniae in a Mouse Pneumococcal Infection Model. Scandinavian Journal of Immunology, 77, 372-388. https://doi.org/10.1111/sji.12047
Togola, A., et al. (2008) Polysaccharides with Complement Fixing and Macrophage Stimulation Activity from Opilia celtidifolia, Isolation and Partial Characterisation. Journal of Ethnopharmacology, 115, 423-431. https://doi.org/10.1016/j.jep.2007.10.017
Magid, A.A., Abdellah, A., Pecher, V., Pasquier, L., Harakat, D. and Voutquenne-Nazabadioko, L. (2017) Flavonol Glycosides and Lignans from the Leaves of Opilia amentacea. Phytochemistry Letters, 21, 84-89. https://doi.org/10.1016/j.phytol.2017.05.023
Youl, O., et al. (2023) Phytochemical Screening and Biological Activities of Opilia amentacea Roxb. (Opiliaceae). https://doi.org/10.20944/preprints202306.1502.v1
May, J.C., Wheeler, R.M. and Grim, E. (1989) The Gravimetric Method for the Determination of Residual Moisture in Freeze-Dried Biological Products. Cryobiology, 26, 277-284. https://doi.org/10.1016/0011-2240(89)90023-0
Koala, M., et al. (2021) HPTLC Phytochemical Screening and Hydrophilic Antioxidant Activities of Apium graveolens L., Cleome gynandra L., and Hibiscus sabdariffa L. Used for Diabetes Management. American Journal of Analytical Chemistry, 12, 15-28. https://doi.org/10.4236/ajac.2021.121002
Turkmen, N., Velioglu, Y.S., Sari, F. and Polat, G. (2007) Effect of Extraction Conditions on Measured Total Polyphenol Contents and Antioxidant and Antibacterial Activities of Black Tea. Molecules, 12, 484-496. https://doi.org/10.3390/12030484
Abdel-Hameed, E.S.S. (2009) Total Phenolic Contents and Free Radical Scavenging Activity of Certain Egyptian Ficus Species Leaf Samples. Food Chemistry, 114, 1271-1277. https://doi.org/10.1016/j.foodchem.2008.11.005
Organisation for Economic Co-Operation and Development (OECD) (2001) OECD Guidelines for the Testing of Chemicals. Guideline 423: Acute Oral Toxicity-Acute Toxic Class Method.
Winter, C.A., Risley, E.A. and Nuss, G.W., (1962) Carrageenin-Induced Edema in Hind Paw of the Rat as an Assay for Antiinflammatory Drugs. Experimental Biology and Medicine, 111, 544-547. https://doi.org/10.3181/00379727-111-27849
Malterud, K.E. and Rydland, K. (2000) Inhibitors of 15-Lipoxygenase from Orange Peel. Journal of Agricultural and Food Chemistry, 48, 5576-5580. https://doi.org/10.1021/jf000613v
Belem-Kabré, W.L.M.E., et al. (2021) Phytochemical and Biological Investigations of Extracts from the Roots of Cocos nucifera L. (Arecaceae) and Carica papaya L. (Caricaceae), Two Plants Used in Traditional Medicine. African Journal of Biochemistry Research, 15, 28-35. https://doi.org/10.5897/AJBR2020.1107
Kim, J.L., Kang, Y.H. and Kang, J.S. (2007) Study on Antioxidant Potency of Green Tea by DPPH Method. The FASEB Journal, 21, A726-A727. https://doi.org/10.1096/fasebj.21.5.A726
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. (1999) Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radical Biology and Medicine, 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
Payne, A.C., Mazzer, A., Clarkson, G.J.J. and Taylor, G. (2013) Antioxidant Assays—Consistent Findings from FRAP and ORAC Reveal a Negative Impact of Organic Cultivation on Antioxidant Potential in Spinach But Not Watercress or Rocket Leaves. Food Science & Nutrition, 1, 439-444. https://doi.org/10.1002/fsn3.71
Hinneburg, I., Dorman Damien, H.J. and Hiltunen, R. (2006) Antioxidant Activities of Extracts from Selected Culinary Herbs and Spices. Food Chemistry, 97, 122-129. https://doi.org/10.1016/j.foodchem.2005.03.028
Sombie, P.A.E.D., et al. (2011) Antioxidant and Anti-Inflammatory Activities from Galls of Guiera senegalensis J.F. Gmel (Combretaceae). Research Journal of Medicinal Plants, 5, 448-461. https://doi.org/10.3923/rjmp.2011.448.461
Ratner, B. (2009) The Correlation Coefficient: Its Values Range Between +1/−1, or Do They? Journal of Targeting, Measurement and Analysis for Marketing, 17, 139-142. https://doi.org/10.1057/jt.2009.5
Kuchta, K. and Cameron, S. (2021) Tradition to Pathogenesis: A Novel Hypothesis for Elucidating the Pathogenesis of Diseases Based on the Traditional Use of Medicinal Plants. Frontiers in Pharmacology, 12, Article 705077. https://doi.org/10.3389/fphar.2021.705077
Raja, K.S., Taip, F.S., Azmi, M.M.Z. and Shishir, M.R.I. (2019) Effect of Pre-Treatment and Different Drying Methods on the Physicochemical Properties of Carica papaya L. Leaf Powder. Journal of Saudi Society of Agricultural Sciences, 18, 150-156. https://doi.org/10.1016/j.jssas.2017.04.001
Ramasar, R., Naidoo, Y., Dewir, Y.H. and El-Banna, A.N. (2022) Seasonal Change in Phytochemical Composition and Biological Activities of Carissa macrocarpa (Eckl.) A. DC. Leaf Extract. Horticulturae, 8, Article 780. https://doi.org/10.3390/horticulturae8090780
Das, A.K., Islam, N., Faruk, O., Ashaduzzaman, M. and Dungani, R. (2020) Review on Tannins: Extraction Processes, Applications and Possibilities. South African Journal of Botany, 135, 58-70. https://doi.org/10.1016/j.sajb.2020.08.008
United Nations (2011) Globally Harmonized System of Classification and Labelling of Chemicals (GHS), Fourth Revised Edition. United Nations, New York.
Tesfaye, R., Degu, A., Abebe, B. and Ayalew, H. (2020) Evaluation of Analgesic and Anti-Inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae). Journal of Inflammation Research, 13, 1175-1183. https://doi.org/10.2147/JIR.S285932
Patil, K.R., et al. (2019) Animal Models of Inflammation for Screening of Anti-Inflammatory Drugs: Implications for the Discovery and Development of Phytopharmaceuticals. International Journal of Molecular Sciences, 20, Article 4367. https://doi.org/10.3390/ijms20184367
Shin, S.A., et al. (2020) Phytochemicals as Anti-Inflammatory Agents in Animal Models of Prevalent Inflammatory Diseases. Molecules, 25, Article 5932. https://doi.org/10.3390/molecules25245932
Saleh, H.A., Yousef, M.H. and Abdelnaser, A. (2021) The Anti-Inflammatory Properties of Phytochemicals and Their Effects on Epigenetic Mechanisms Involved in TLR4/NF-κB-Mediated Inflammation. Frontiers in Immunology, 12, Article 606069. https://doi.org/10.3389/fimmu.2021.606069
Nwachukwu, I.D., Sarteshnizi, R.A., Udenigwe, C.C. and Aluko, R.E. (2021) A Concise Review of Current in Vitro Chemical and Cell-Based Antioxidant Assay Methods. Molecules, 26, Article 4865. https://doi.org/10.3390/molecules26164865
Zhang, Y.J., et al. (2015) Antioxidant Phytochemicals for the Prevention and Treatment of Chronic Diseases. Molecules, 20, 21138-21156. https://doi.org/10.3390/molecules201219753
Christodoulou, M.C., et al. (2022) Spectrophotometric Methods for Measurement of Antioxidant Activity in Food and Pharmaceuticals. Antioxidants Basel, 11, Article 2213. https://doi.org/10.3390/antiox11112213
Jacobo-Velázquez, D.A. and Cisneros-Zevallos, L. (2009) Correlations of Antioxidant Activity against Phenolic Content Revisited: A New Approach in Data Analysis for Food and Medicinal Plants. Journal of Food Sciences, 74, R107-R113. https://doi.org/10.1111/j.1750-3841.2009.01352.x