The Protective Effects of Flavonoids from <i>Scutellaria Baicalensis</i> Georgi Stems and Leaves on Oligodendrocyte Damage Induced by A<i>β</i><sub>1-42</sub> — Oak Academic Publishing
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The Protective Effects of Flavonoids from <i>Scutellaria Baicalensis</i> Georgi Stems and Leaves on Oligodendrocyte Damage Induced by A<i>β</i><sub>1-42</sub>
Institute of Traditional Chinese Medicine, Chengde Medical College, Chengde, China
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Institute of Traditional Chinese Medicine, Chengde Medical College, Chengde, China
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Hebei Province Key Research Office of Traditional Chinese Medicine against Dementia, Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development, Hebei Key Laboratory of Nerve Injury and Repair, Chengde, China
1 Institute of Traditional Chinese Medicine, Chengde Medical College, Chengde, China
2 Institute of Traditional Chinese Medicine, Chengde Medical College, Chengde, China
3 Hebei Province Key Research Office of Traditional Chinese Medicine against Dementia, Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development, Hebei Key Laboratory of Nerve Injury and Repair, Chengde, China
Aim: This study aimed to investigate the protective effects of flavonoids from the stem and leaves of Scutellaria baicalensis Georgi (SSFs) against A β 1-42 -induced oligodendrocytes (OL) damage. Methods: Immunofluorescence was used for the detection of myelin-associated glycoprotein (MAG), a characteristic protein of rat oligodendrocytes (OLN-93 cells). To evaluate the potential protective effects of SSFs on OLN-93 cells injured by A β 1-42 , an injury model was established by subjecting OLN-93 cells to A β 1-42 exposed. Cell morphology was examined using an inverted microscope, while cell viability was assessed using the colorimetric method of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Additionally, lactate dehydrogenase (LDH) was measured using the pyruvic acid reduction assay. The Ginkgo biloba leaf extract (GBE) injection was used as a positive control. Results: A total of >95% of the MAG immunofluorescence-positive cells were identified as oligodendrocytes. Gradually increasing concentrations of SSFs impaired the cells, and the maximum nondetrimental dose for OLN-93 cells was 75 mg/L. This study assessed the effects of SSFs on OLN-93 cells damaged by A β 1-42 . The results indicated that SSFs significantly improved OLN-93 cell morphological abnormal changes, increased the OLN-93 cell survival rate, and reduced LDH release. Conclusion: SSFs can alleviate A β 1-42 -induced damage of OL.
KeywordsFlavonoids form the Stem and Leaves of <i>Scutellaria baicalensis</i>GeorgiA<i>β</i><sub>1-42</sub>
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