Research ArticleOpen AccessGoogle Scholar indexed
A Novel Capillary Zone Electrophoresis Technique for Pharmacokinetic Studies on Porphyrin-C 60 Based Neuroprotectors. Preliminary Report
Institute of Biomedicine, N.I. Pirogov Russian National Research Medical University, Moscow, Russian Federation
Institute of Biomedicine, N.I. Pirogov Russian National Research Medical University, Moscow, Russian Federation
Institute of Biomedicine, N.I. Pirogov Russian National Research Medical University, Moscow, Russian Federation
Institute of Biomedicine, N.I. Pirogov Russian National Research Medical University, Moscow, Russian Federation
Institute of Biomedicine, N.I. Pirogov Russian National Research Medical University, Moscow, Russian Federation
- 1 Institute of Biomedicine, N.I. Pirogov Russian National Research Medical University, Moscow, Russian Federation
- 2 Institute of Biomedicine, N.I. Pirogov Russian National Research Medical University, Moscow, Russian Federation
- 3 Institute of Biomedicine, N.I. Pirogov Russian National Research Medical University, Moscow, Russian Federation
- 4 Institute of Biomedicine, N.I. Pirogov Russian National Research Medical University, Moscow, Russian Federation
- 5 Institute of Biomedicine, N.I. Pirogov Russian National Research Medical University, Moscow, Russian Federation
Pharmacology & Pharmacy·Volume 16 (2025)·Pages 165–173·Published 18 June 2025·DOI10.4236/pp.2025.166011
Copy link · social · email
Abstract
A novel, fast-n-reliable, Quartz capillary silica zone electrophoresis required to upgrade an arsenal of analytical tools for current pharmacokinetics studies on porphyrin-fullerene nanoparticles has been proposed. The fullerene ( C 60 ) -tetra ( p- hydroxyphenyl ) porphyrin structures, known for their capabilities to paramagnetic 25 Mg 2+ delivery, were found quantitatively detectable in a total rat brain cytosol (S125) fraction, once these nanocationites administered in a single, either 1.0 mg/kg or 20.0 mg/kg, i.v. injection followed by 12 hrs long animal-drug exposition time. Driven by pressing needs in ongoing preclinical trial platform developments, the CZE track proposed might make an impact on both convenience and efficiency of pharmacokinetic estimate of medicinal nanocationites.
KeywordsNeuroprotectorsPorphyrin-FullerenesIschaemic Brain StrokePreclinical TrialCapillary Zone Electrophoresis (CZE)
- Rezayat, S.M., Boushehri, S.V.S., Salmanian, B., Omidvari, A.H., Tarighat, S., Esmaeili, S., et al . (2009) The Porphyrin-Fullerene Nanoparticles to Promote the ATP Overproduction in Myocardium: 25 Mg 2+ -Magnetic Isotope Effect. European Journal of Medicinal Chemistry , 44, 1554-1569. https://doi.org/10.1016/j.ejmech.2008.07.030
- Mironov, A.F. (2011) Synthesis, Properties, and Potential Applications of Porphyrin-fullerenes. Macroheterocycles , 4, 186-208. https://doi.org/10.6060/mhc2011.3.08
- Karmova, F.M., Lebedeva, V.S. and Mironov, A.F. (2016) Fullerene-Containing Porphyrins: Synthesis and Potential Practical Applications. Russian Journal of General Chemistry , 86, 2145-2179. https://doi.org/10.1134/s1070363216090322
- Kazemzadeh, H. and Mozafari, M. (2019) Fullerene-Based delivery systems. Drug Discovery Today , 24, 898-905. https://doi.org/10.1016/j.drudis.2019.01.013
- Amirshahi, N., Alyautdin, R.N., Sarkar, S., Rezayat, S.M., Orlova, M.A., Trushkov, I.V., et al . (2008) Fullerene-Based Low Toxic Nanocationite Particles (Porphyrin Adducts of Cyclohexyl Fullerene-C 60 ) to Treat Hypoxia-Induced Mitochondrial Dysfunction in Mammalian Heart Muscle. Archives of Medical Research , 39, 549-559. https://doi.org/10.1016/j.arcmed.2008.05.007
- (2013) Patent CN103193786A: Water-Soluble Fullerene—Porphyrin, Preparation Method and Application Thereof.
- Buchachenko, A.L. (2015) Magneto-Biology and Medicine. Nova Biomedical Publishers Inc.
- Buchachenko, A.L., Bukhvostov, A.A., Ermakov, K.V. and Kuznetsov, D.A. (2020) A Specific Role of Magnetic Isotopes in Biological and Ecological Systems. Physics and Biophysics Beyond. Progress in Biophysics and Molecular Biology , 155, 1-19. https://doi.org/10.1016/j.pbiomolbio.2020.02.007
- Buchachenko, A.L. and Kuznetsov, D.A. (2014) Magnetic Control of Enzymatic Phosphorylation. Journal of Physical Chemistry & Biophysics , 2, Article ID: 1000142. https://doi.org/10.4172/2161-0398.1000142
- Koltover, V.K. (2014) Stable Magnetic Isotopes: From Spin Chemistry to Biomedicine. Russian Chemical Bulletin , 63, 1029-1035. https://doi.org/10.1007/s11172-014-0545-3
- Knape, M.J., Ballez, M., Burghardt, N.C., Zimmermann, B., Bertinetti, D., Kornev, A.P., et al . (2017) Divalent Metal Ions Control Activity and Inhibition of Protein Kinases. Metallomics , 9, 1576-1584. https://doi.org/10.1039/c7mt00204a
- Buchachenko, A., Bukhvostov, A., Ermakov, K. and Kuznetsov, D. (2019) Nuclear Spin Selectivity in Enzymatic Catalysis: A Caution for Applied Biophysics. Archives of Biochemistry and Biophysics , 667, 30-35. https://doi.org/10.1016/j.abb.2019.04.005