Anti-Inflammatory Activity of Hydro-Ethanolic Extract of Maytenus senegalensis Lam ( Celastraceae ) Roots and Leaves and Their Fractions — Oak Academic Publishing
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Anti-Inflammatory Activity of Hydro-Ethanolic Extract of Maytenus senegalensis Lam ( Celastraceae ) Roots and Leaves and Their Fractions
Laboratory of Pharmacology, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
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Laboratory of Pharmacology, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
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Laboratory of Pharmacognosy and Botany, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
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Laboratory of Pharmacology, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
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Laboratory of Pharmacology, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
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Laboratory of Pharmaceutical Physiology, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
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Laboratory of Pharmacology, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
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Laboratory of Pharmacognosy and Botany, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
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Laboratory of Pharmacology, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
1 Laboratory of Pharmacology, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
2 Laboratory of Pharmacology, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
3 Laboratory of Pharmacognosy and Botany, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
4 Laboratory of Pharmacology, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
5 Laboratory of Pharmacology, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
6 Laboratory of Pharmaceutical Physiology, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
7 Laboratory of Pharmacology, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
8 Laboratory of Pharmacognosy and Botany, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
9 Laboratory of Pharmacology, Faculty of Medicine, Pharmacy and Odonto-Stomatology, Cheikh Anta Diop University, Dakar-Fann, Senegal
Maytenus senegalensis ( Celastraceae ) is a plant in the African pharmacopoeia and is widely used in traditional medicine in various pathologies associated to inflammation and pain. The aim of this study was to evaluate the anti-inflammatory activity of hydro-ethanolic extract of Maytenus senegalensis roots and leaves and their fractions in rats. Extraction with ethanol and liquid/liquid fractionations were carried out. Characterization reactions were performed to identify the chemical compounds. The anti-inflammatory activity of hydro-ethanolic extract of Maytenus senegalensis roots (HEE R ) and leaves (HEE L ) and their ethyl acetate (EAF R , EAF L ) and dichloromethane (DF R , DF L ) fractions was investigated on a pharmacological model of carrageenan-induced acute edema of the rat paw in comparison to that of aspirin used as a reference substance. Phytochemical study revealed the presence of tannins, flavonoids, sterols and triterpenes in the extracts. HEE R , HEE L and their fractions at doses of 10 and 30 mg/kg per os and the reference product (30 mg/kg) significantly prevented rat paw edema associated with carrageenan injection from T1H to T5H. HEE R at 30 mg/kg was more effective in preventing paw edema in rats than at 10 mg/kg, its fractions EAF R and DF R (10 and 30 mg/kg) and aspirin (30 mg/kg). At 10 mg/kg, HEE L showed a greater preventive effect on inflammatory edema than at 30 mg/kg, but less than its fractions (EAF L and DF L ). HEE R exhibits greater anti-inflammatory activity than HEE F at dose of 30 mg/kg, but at 10 mg/kg, HEE F is slightly more effective than HEE R , particularly at T1H and T5H. The results indicate that extracts of Maytenus senegalensis roots and leaves contain chemical substances with anti-inflammatory properties and could contribute to the treatment of inflammatory diseases.
Hardmann, J.A., Limbard, L.E. and Goodmann, A. (1998) Pharmacological Basis of Therapeutics. McGraw Hill, 1465.
Sahlmann, C. and Ströbel, P. (2016) Pathophysiology of Inflammation. Nuklearme-dizin. Nuklearmedizin , 55, 1-6. https://doi.org/10.1055/s-0037-1616468
Thapa, R., Afzal, O., Alfawaz Altamimi, A.S., Goyal, A., Almalki, W.H., Alzarea, S.I., et al . (2023) Galangin as an Inflammatory Response Modulator: An Updated Overview and Therapeutic Potential. Chemico - Biological Interactions , 378, Article 110482. https://doi.org/10.1016/j.cbi.2023.110482
Viladomiu, M., Hontecillas, R. and Bassaganya-Riera, J. (2016) Modulation of Inflammation and Immunity by Dietary Conjugated Linoleic Acid. European Journal of Pharmacology , 785, 87-95. https://doi.org/10.1016/j.ejphar.2015.03.095
Chiolero, A., Würzner, G. and Burnier, M. (2000) Selective Inhibitors of Type 2 Cyclooxygenase: Less Renal Effects than the Classical Non-Steroidal Anti-Inflammatory Agents. Néphrologie , 21, 425-430.
Harirforoosh, S., Asghar, W. and Jamali, F. (2014) Adverse Effects of Nonsteroidal Antiinflammatory Drugs: An Update of Gastrointestinal, Cardiovascular and Renal Complications. Journal of Pharmacy & Pharmaceutical Sciences , 16, 821-847. https://doi.org/10.18433/j3vw2f
Soubrier, M., Rosenbaum, D., Tatar, Z., Lahaye, C., Dubost, J. and Mathieu, S. (2013) Anti-Inflammatoires non stéroïdiens et vaisseaux. Revue du Rhumatisme , 80, 204-208. https://doi.org/10.1016/j.rhum.2012.11.012
Kokwaro, J.O. (1976) Medicinal Plants of East Africa. East African Literature Bureau.
Malebo, H.M., Wiketye, V., Katani, S.J., Kitufe, N.A., Nyigo, V.A., Imeda, C.P., et al . (2015) In Vivo Antiplasmodial and Toxicological Effect of Maytenus senegalensis Traditionally Used in the Treatment of Malaria in Tanzania. Malaria Journal , 14, Article No. 79. https://doi.org/10.1186/s12936-014-0525-y
Kerharo, J. and Adam, J.G. (1964) Plantes médicinales et toxiques des Peul et des Toucouleur du Sénégal. Journal D ’ agriculture Tropicale et de Botanique Appliquée , 11, 543-599. https://doi.org/10.3406/jatba.1964.2795
Gessler, M.C., Nkunya, M.H.H., Mwasumbi, L.B., Heinrich, M. and Tanner, M. (1994) Screening Tanzanian Medicinal Plants for Antimalarial Activity. Acta Tropica , 56, 65-77. https://doi.org/10.1016/0001-706x(94)90041-8
Gessler, M.C., Tanner, M., Chollet, J., Nkunya, M.H.H. and Heinrich, M. (1995) Tanzanian Medicinal Plants Used Traditionally for the Treatment of Malaria: in Vivo Antimalarial and in Vitro Cytotoxic Activities. Phytotherapy Research , 9, 504-508. https://doi.org/10.1002/ptr.2650090708
El Tahir, A., Satti, G.M.H. and Khalid, S.A. (1999) Antiplasmodial Activity of Selected Sudanese Medicinal Plants with Emphasis on Maytenus senegalensis (Lam.) Exell. Journal of Ethnopharmacology , 64, 227-233. https://doi.org/10.1016/s0378-8741(98)00129-9
Tahir, A.E., Ibrahim, A.M., Satti, G.M.H., Theander, T.G., Kharazmi, A. and Khalid, S.A. (1998) The Potential Antileishmanial Activity of Some Sudanese Medicinal Plants. Phytotherapy Research , 12, 576-579.
Matu, E.N. and van Staden, J. (2003) Antibacterial and Anti-Inflammatory Activities of Some Plants Used for Medicinal Purposes in Kenya. Journal of Ethnopharmacology , 87, 35-41. https://doi.org/10.1016/s0378-8741(03)00107-7
Sanogo, R., Diallo, D., Maiga, A., De Tommasi, N. and De Pasquale, R. (2006) Activités analgésique et anti-inflammatoire des extraits de Maytenus senegalensis, Stereospermum kunthianum et Trichilia emetica utilisés dans le traitement traditionnel des dysménorrhées au Mali. Planta Medica , 72, 123-136. https://doi.org/10.1055/s-2006-950058
Kpoyizoun, P.K., Metowogo, K., Kantati, Y.T., Missebukpo, A., Dare, T., Lawson-Evi, P., et al . (2020) Antiinflammatory and Antioxidant Evaluation of Maytenus Senegalensis Hydroalcoholic Roots Extract Fractions in Allergic Asthma. The Journal of Phytopharmacology , 9, 252-257. https://doi.org/10.31254/phyto.2020.9407
SALL, C., Seck, M., Faye, B., Dioum, M.D., Seck, I., Gueye, P.M., et al . (2016) In Vitro Study of Anti-Sickling Effect of Red Blood Cells and the Antioxidant Activity of Extracts of Roots Powder of Maytenus senegalensis Lam (Celastraceae). International Journal of Biological and Chemical Sciences , 10, Article 1017. https://doi.org/10.4314/ijbcs.v10i3.9
Tin-Wa, M., Farnsworth, N.R., Fong, H.H., Blomster, R.N., Trojánek, J., et al . (1971) Biological and Phytochemical Evaluation of Plants. IX. Antitumor Activity of Ma y tenus senegalensis (Celastraceae) and a Preliminary Phytochemical Investigation. Lloydia , 34, 79-87.
Otake, T., Mori, H., Morimoto, M., Ueba, N., Sutardjo, S., Kusumoto, I.T., et al . (1995) Screening of Indonesian Plant Extracts for Anti‐Human Immunodeficiency Virus—Type 1 (HIV‐1) Activity. Phytotherapy Research , 9, 6-10. https://doi.org/10.1002/ptr.2650090103
Hussein, G., Miyashiro, H., Nakamura, N., Hattori, M., Kawahata, T., Otake, T., et al . (1999) Inhibitory Effects of Sudanese Plant Extracts on HIV-1 Replication and HIV-1 Protease. Phytotherapy Research , 13, 31-36. https://doi.org/10.1002/(sici)1099-1573(199902)13:1<31::aid-ptr381>3.0.co;2-c
Hussein, G., Nakamura, N., Meselhy, M.R. and Hattori, M. (1999) Phenolics from Maytenus Senegalensis. Phytochemistry , 50, 689-694. https://doi.org/10.1016/s0031-9422(98)00571-8
Okine, L.K.N., Nyarko, A.K., Osei-Kwabena, N., Oppong, I.V., Barnes, F. and Ofosuhene, M. (2005) The Antidiabetic Activity of the Herbal Preparation ADD-199 in Mice: A Comparative Study with Two Oral Hypoglycaemic Drugs. Journal of Ethnopharmacology , 97, 31-38. https://doi.org/10.1016/j.jep.2004.09.048
Umar, S.I., Ndako, M., Jigam, A.A., Adefolalu, S.F., Ibikunle, G.F. and Lawal, B. (2019) Anti-Plasmodial, Anti-Inflammatory, Anti-Nociceptive and Safety Profile of Maytenus Senegalensis Root Bark Extract on Hepato-Renal Integrity in Experimental Animals. Comparative Clinical Pathology , 28, 1571-1579. https://doi.org/10.1007/s00580-019-02965-4
Huang, Y., Chen, L., Ma, G., Xu, X., Jia, X., Deng, F., et al . (2021) A Review on Phytochemicals of the Genus Maytenus and Their Bioactive Studies. Molecules , 26, Article 4563. https://doi.org/10.3390/molecules26154563
Bah, F., Kane, Y., Daffé, M.L., Touré, A., Lam, A., Cabral, M., Seck, M. and Fall, M. (2021) Acute and Subacute Toxicity in Wistar Rats of the Methanolic Extract of the Roots of Maytenus senegalensis (Lam.) Exell. Journal of Pharmaceutical and Biological Sciences , 16, 1-8.
Joslyn, M.A. (1970) Methods in Food Analysis. Physical, Chemical and Instrumental Methods of Analysis. Academic Press.
Harborne, J.B. (1998) Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. Springer Science & Business Media.
Winter, C.A., Risley, E.A. and Nuss, G.W. (1962) Carrageenin-Induced Edema in Hind Paw of the Rat as an Assay for Antiinflammatory Drugs. Experimental Biology and Medicine , 111, 544-547. https://doi.org/10.3181/00379727-111-27849
Vinegar, R., Schreiber, W. and Hugo, R. (1969) Biphasic Development of Carrageenin Edema in Rats. The Journal of Pharmacology and Experimental Therapeutics , 166, 96-103. https://doi.org/10.1016/s0022-3565(25)28209-x
Crunkhorn, P. and Meacock, S.C.R. (1971) Mediators of the Inflammation Induced in the Rat Paw by Carrageenin. British Journal of Pharmacology , 42, 392-402. https://doi.org/10.1111/j.1476-5381.1971.tb07124.x
Di Rosa, M., Giroud, J.P. and Willoughby, D.A. (1971) Studies of the Mediators of the Acute Inflammatory Response Induced in Rats in Different Sites by Carrageenan and Turpentine. The Journal of Pathology , 104, 15-29. https://doi.org/10.1002/path.1711040103
Holsapple, M.P., Schnur, M. and Yim, G.K.W. (1980) Pharmacological Modulation of Edema Mediated by Prostaglandin, Serotonin and Histamine. Agents and Actions , 10, 368-373. https://doi.org/10.1007/bf01971442
Morris, C.J. (2003) Carrageenan-Induced Paw Edema in the Rat and Mouse. In: Winyard, P.G. and Willoughby, D.A., Eds., Inflammation Protocols , Humana Press, 115-122. https://doi.org/10.1385/1-59259-374-7:115
Khan, I., Nisar, M., Ebad, F., Nadeem, S., Saeed, M., Khan, H., et al . (2009) Anti-inflammatory Activities of Sieboldogenin from Smilax China Linn.: Experimental and Computational Studies. Journal of Ethnopharmacology , 121, 175-177. https://doi.org/10.1016/j.jep.2008.10.009
Bhukya, B., Anreddy, R.N.R., William, C.M. and Gottumukkala, K.M. (2009) Analgesic and Anti-Inflammatory Activities of Leaf Extract of Kydia Calycina Roxb. Bangladesh Journal of Pharmacology , 4, 101-104. https://doi.org/10.3329/bjp.v4i2.2112
Nantel, F., Denis, D., Gordon, R., Northey, A., Cirino, M., Metters, K.M., et al . (1999) Distribution and Regulation of Cyclooxygenase‐2 in Carrageenan‐Induced Inflammation. British Journal of Pharmacology , 128, 853-859. https://doi.org/10.1038/sj.bjp.0702866
Oates, J.A., Wood, A.J.J., Brooks, P.M. and Day, R.O. (1991) Nonsteroidal Anti-Inflammatory Drugs—Differences and Similarities. New England Journal of Medicine , 324, 1716-1725. https://doi.org/10.1056/nejm199106133242407
Al-Khayri, J.M., Sahana, G.R., Nagella, P., Joseph, B.V., Alessa, F.M. and Al-Mssallem, M.Q. (2022) Flavonoids as Potential Anti-Inflammatory Molecules: A Review. Molecules , 27, Article 2901. https://doi.org/10.3390/molecules27092901
Shamsudin, N.F., Ahmed, Q.U., Mahmood, S., Shah, S.A.A., Sarian, M.N., Khattak, M.M.A.K., et al . (2022) Flavonoids as Antidiabetic and Anti-Inflammatory Agents: A Review on Structural Activity Relationship-Based Studies and Meta-Analysis. International Journal of Molecular Sciences , 23, Article 12605. https://doi.org/10.3390/ijms232012605
Thomas, M.L.R.M.G. and Filho, J.M.B. (1985) Anti-Inflammatory Actions of Tannins Isolated from the Bark of Anacardwm occidentale L. Journal of Ethnopharmacology , 13, 289-300. https://doi.org/10.1016/0378-8741(85)90074-1
Choi, J., Huh, K., Kim, S., Lee, K., Park, H. and Han, Y.N. (2002) Antinociceptive and Anti-Rheumatoidal Effects of Kalopanax Pictus Extract and Its Saponin Components in Experimental Animals. Journal of Ethnopharmacology , 79, 199-204. https://doi.org/10.1016/s0378-8741(01)00383-x
Shu, P., Li, L., He, Q., Pan, J., Li, X., Zhu, M., et al . (2021) Identification and Quantification of Oleanane Triterpenoid Saponins and Potential Analgesic and Anti-Inflammatory Activities from the Roots and Rhizomes of Panax Stipuleanatus. Journal of Ginseng Research , 45, 305-315. https://doi.org/10.1016/j.jgr.2020.05.002
Amnay, A., Ouriagli, T., Raoui, S.M., Errachidi, F., Chahdi, F.O., Bennani, B., Rodi, Y.K. and Rachida, C.R. (2023) Antioxidant and Anti-Inflammatory Activities of Sap-onins Extracted from Marrubium vulgare L. Collected in Fez-Meknes Region in Morocco. Tropical Journal of Natural Product Research , 7, 3613-3619.
Wang, H., Gao, J., Kou, J., Zhu, D. and Yu, B. (2008) Anti-Inflammatory Activities of Triterpenoid Saponins from Polygala Japonica. Phytomedicine , 15, 321-326. https://doi.org/10.1016/j.phymed.2007.09.014
Akihisa, T., Yasukawa, K., Kimura, Y., Takase, S., Yamanouchi, S. and Tamura, T. (1997) Triterpene Alcohols from Camellia and Sasanqua Oils and Their Anti-Inflammatory Effects. Chemical and Pharmaceutical Bulletin , 45, 2016-2023. https://doi.org/10.1248/cpb.45.2016
Souto, A.L., Tavares, J.F., Da Silva, M.S., Diniz, M.d.F.F.M., De Athayde-Filho, P.F. and Barbosa Filho, J.M. (2011) Anti-Inflammatory Activity of Alkaloids: An Update from 2000 to 2010. Molecules , 16, 8515-8534. https://doi.org/10.3390/molecules16108515