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Radioiodination of Cephalexin with <sup>125</sup>I and Its Biological Behavior in Mice
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Pharmacology & Pharmacy·Volume 03 (2012)·Pages 97–102·Published 12 January 2012·DOI10.4236/pp.2012.31014
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Abstract
A procedure for radioiodination of Cephalexin with iodine-125 was carried out via an electrophilic substitution reaction. Using 3.7 MBq of Na 125 I, 200μl of cephalexin as substrate, 200 μl of iodogen as oxidizing agent in acetone at 40°C for 20 min, a maximum radiochemical yield of 125 I-Ceph (95%) was obtained. The labeled compound was separated and purified by means of high-pressure liquid chromatography (HPLC). The biological distribution in normal and inflamed (septic and aseptic) mice indicates the suitability of radioiodinated cephalexin for imaging of inflammation
KeywordsCephalexinNa<sup>125</sup>IElectrophilic Substitution ReactionRadioiodinationInflammation
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