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Research of Quality Standards for Stachydrine Hydrochloride in Chinese Medicine TJF Granule

¹ Faculty of Sciences, School of Biomedical Sciences, University of Nottingham (Malaysia Campus), Semenyih, Malaysia
² National Engineering Research Center for Traditional Chinese Medicine, Shanghai, China.
³ National Engineering Research Center for Traditional Chinese Medicine, Shanghai, China.
⁴ Faculty of Sciences, School of Biomedical Sciences, University of Nottingham (Malaysia Campus), Semenyih, Malaysia
⁵ Faculty of Sciences, School of Biomedical Sciences, University of Nottingham (Malaysia Campus), Semenyih, Malaysia

Pharmacology & Pharmacy·Volume 04 (2013)·Pages 277–282·Published 31 May 2013·DOI10.4236/pp.2013.43040

Abstract

A high-performance liquid chromatographic method was applied to the determination of stachydrine hydrochloride concentration in TJF granule (Chinese name: Tiao-Jing-Fang), using a mobile phase of methanol - acetonitrile (50:50, v/v) by the Agilent Kromasi NH 2 column (250 mm × 4.6 mm, 5 μ m, S/N: 22N25110). Detection wavelength was 201 nm. The result revealed good linearity of stachydrine hydrochloride and was obtained within the range of 0.20 - 1.98 μ g/mL (R = 0.9995). The average recovery was 97.01%; the relative standard deviation (RSD) was 0.19%. To the best of our knowledge, this is the first report dedicated to the determination of stachydrine hydrochloride by the e vaporative l ight s cattering d etector - high-performance liquid chromatographic (ELSD-HPLC) method.

KeywordsTJF GranuleQuality StandardsTraditional Chinese MedicineEvaporative Light Scattering Detector (ELSD)High-Performance Liquid Chromatographic (HPLC) MethodStachydrine HydrochloridePharmaceutical AnalysisDetermination

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Research of Quality Standards for Stachydrine Hydrochloride in Chinese Medicine TJF Granule

Abstract

References (12)