T here is scarce information about the effects of danazol and its derivatives at cardiovascular level. In addition, to date the cellular site and mechanism of action of danazol at the cardiovascular level is very confusing. In order to clarify those phenomena in this study, a danazol derivative was synthesized with the objective of evaluat ing its activity on per fusion pressure and coronary resistance and compar ing this phenomenon with the effect exerted by danazol . T he Lan gendorff technique was used to measure changes on perfusion pressure and coronary resistance in an isolated rat heart model in the absence or presence of danazol and its derivative. Additionally, to characterize the molecular mechanism involved in the inotropic activity induced by danazol derivative was evaluated by measuring left ventricular pressure in the absence or presence of following compounds; flutamide, prazosin, metoprolol, indomethacin and nifedipine. The results showed that danazol derivative significantly increased the perfusion pressure and coronary resistance in com parison with the control conditions and danazol . Additionally, other data indicate that the danazol derivative increase s left ventricular pressure in a dose-dependent manner; nevertheless, this phenomenon was significantly inhibited by flu tamide. These data suggest that danazol derivative induces positive inotropic activity through of the activation androgen receptor.
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