Aims: This paper investigates the presence of M 3 muscarinic acetylcholine receptor autoantibody present in the serum of patients with primary SjÖgren syndrome (pSS). Main methods: We detected the levels of M 3 mAChR peptide IgG, PGE 2 , IL-1β in serum of SS patients using the enzyme-linked immune sorbent assay (ELISA). To measure the quantity of nitrite/nitrate, we used Griess reagent system. Key findings: Titres of M 3 mAChR antibody in sera from SS patients are significantly enhanced compared to healthy subjects (control). The enhancement of these autoantibodies is accompanied by the increase of the levels of PGE 2 , IL-1β and nitrite/nitrate in serum. Under in vitro conditions, the synthetic human M 3 peptide impaires the increment of M 3 mAChR antibody but not that of nati-Ro/SSA antibody. In positive anti-Ro/SSA antibody patients, the increment of M 3 mAChR peptide IgG and the measured pro-inflammatory substances is related. Significance: On this basis, anti M 3 mAChR peptide IgG can be said to act as a modulator of the immune system and to play a role in the host-chronic increment of proinflammatory substances in SS patients with positive Ro/SSA antibody. This association between the antibody and the pathogenesis of SS disease may result in useful predicting SS.
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