Study on the Multi-Component Quantitation of Cortex Moutan and Its Intestinal Absorption Characteristics
- 1 College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China
- 2 College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China
- 3 College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China
- 4 College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China
- 5 College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China
- 6 College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China
- 7 College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China
- 8 College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China
Abstract
Cortex Moutan ( Paeonia suffruticosa Andr.) is a common traditional Chinese medicine and has been widely used in clinic for 2000 years in China. As sources for this crude drug are always mixed with other species , many cultivars on herbal market may lead to quality instability. Multi-component quantitative analysis is an efficient method to reflect chemical profiles of herb medicine and is always taken as the main method for quality evaluation. So , the aim of this work is to develop analytical method to quantify paeonol, paeoniflorin, gallic acid, oxypaeoniflorin, benzoylpaeoniflorin and paeonolide in Cortex Moutan (CM) to evaluate the chemical qualities of CM from different species or cultivars. Besides, we also study the intestinal absorption characteristics of paeonol and paeoniflorin for further pharmacological evaluation. In the present study, all of the standard markers were performed on an Ecosil C 18 (250 mm × 4.6 mm, 5 μM, Lubex Co., Guangzhou, China) with linear gradient elution of 0.2% formic acid water and acetonitrile. The proposed method was applied to analyze 50 batches of samples with acceptable linearity (R 2 , 0.9995 - 0.9999), precisions (RSD, 0.47% - 2.08%), repeatability (RSD, 039% - 2.63%), stability (RSD, 0.52% - 2.45%), and recovery (RSD, 0.72% - 3.03%) of the six compounds. Furthermore, the Hierarchical Cluster Analysis was applied to classify the 50 samples based on contents of the six compound markers. The results obtained from multi-component quantification of CM clearly indicated that CM originated from P. suffruticosa and P. ostii presented different chemical properties, and that samples from the two materials could be gathered into one branch, respectively , w hile CM sourced from cultivars of P. suffruticosa showed great variety on chemical quality. The results from Hierarchical Cluster Analysis implied that the established method could be used as a powerful tool for the quality evaluation of CM. The intestinal absorption study indicated that the intestinal absorption activities for paeoniflorin and paeonol showed an increasing absorption with time. Paeonol had lower absorption rate (6.69% - 15.93%) than that of paeoniflorin (19.0% - 30.70%). As a result, the established method is suitable for the quality evaluation of CM. The results of intestinal absorption characteristics of paeonol and paeoniflorin offer an insight for pharmacological evaluation and clinical efficacy research of CM.
- Zhang, Y.X., Yu, D., Liu, C.Y. and Gai, S.P. (2018) Dynamic of Carbohydrate Metabolism and the Related Genes Highlights PPP Pathway Activation during Chilling Induced bud Dormancy Release in tree Peony (Paeonia suffruticosa). Scientia Horticulturae, 242, 36-43. https://doi.org/10.1016/j.scienta.2018.07.022
- Chinese Pharmacopeia Commission (2015) Pharmacopoeia of the People’s Republic of China. Vol. 1, 172.
- Peng, H.S., Wang, D.Q., Peng, D.Y. and Huang, L.Q. (2017) Research and Investigation on Original Plants of Medicinal Moutan. China Journal of Chinese Material Medica, 42, 1632-1636.
- Qiu, Y.X, Huang, J.H., Jiang, X.M., Chen, Y., Liu, Y., Zeng, R., Shehla, N., Liu, Q., Liao, D.F., Guo, D., Liang, Y.Z. and Wang, W. (2015) Quantitative and Qualitative Determination of LiuweiDihuang Preparations by Ultra High Performance Liquid Chromatography in Dual-Wavelength Fingerprinting Mode and Random Forest. China Journal of Chinese Materia Medica, 38, 3720-3726.
- Wang, S.Y., Huang, J., Mao, H.J., Wang, Y.L., Kasimu, R., Wei, X. and Wang, J.H. (2014) A Novel Method HPLC-DAD Analysis of the Contents of Moutan Cortexand Paeoniae Radix Alba with Similar Constituents-Monoterpene Glycosides in Guizhi Fuling Wan. Molecules, 19, 17957-17967. https://doi.org/10.3390/molecules191117957
- Luo, S., Wen, R.Y., Wang, Q., Zhao, Z.X., Nong, F.F., Fu, Y.J., Huang, S.W., Chen, J.Y., Zhou, L. and Luo, X. (2019) Rhubarb Peony Decoction Ameliorates Ulcerative Colitis in Mice by Regulating Gut Microbiota to Restoring Th17/Treg Balance. Journal of Ethnopharmacology, 231, 39-49. https://doi.org/10.1016/j.jep.2018.08.033
- Guo, B.L., Basang, D.J., Xiao, P.G. and Hong, D.Y. (2002) Research on the Quality of Original Plants and Material Medicine of Cortex Paeoniae. China Journal of Chinese Material Medica, 27, 654-657.
- Li, L.Z.; Song, N. and Wang, X.L. (2014) Studies on Mechanism of Cortex Moutan on Removing Heat to Cool and Promoting Blood Circulation to Diminish Carbuncle. Chinese Archives of Traditional Chinese Medicine, 32, 863-865.
- Ding, L.Q., Jiang, Z.H., Liu, Y., Chen, L.X., Zhao, Q., Yao, X.S., Zhao, F. and Qiu, F. (2012) Monoterpenoid Inhibitors of NO Production from Paeonia suffruticosa. Fitoterapia, 83, 1598-1603. https://doi.org/10.1016/j.fitote.2012.09.008
- Ding, L.Q., Zhao, F., Chen, L.X., Jiang, Z.H., Liu, Y., Li, Z.M., Qiu, F. and Yao, X.S. (2012) New Monoterpene Glycosides from Paeonia suffruticosa Andrews and Their Inhibition on NO Production in LPS-Induced RAW 264.7 Cells. Bioorganic & Medicinal Chemistry Letters, 22, 7243-7247. https://doi.org/10.1016/j.bmcl.2012.09.034