Expression of NSE and AFI in Fatal Lamb’s Lungs before and after Relieving Ureteral Obstruction
- 1 Department of Pediatric Surgery, Guangzhou Women and Children’s Medical Center, Guangzhou, China
- 2 Department of Pediatric Surgery, Guangzhou Women and Children’s Medical Center, Guangzhou, China
- 3 Department of Pediatric Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
- 4 Department of Pediatric Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
- 5 Department of Pediatric Surgery, Guangzhou Women and Children’s Medical Center, Guangzhou, China
- 6 Department of Pediatric Surgery, Guangzhou Women and Children’s Medical Center, Guangzhou, China
Abstract
Aims: to evaluate the Expression of NSE and AFI before and after relieving ureteral obstruction in fatal lamb’s lungs with unilateral hydronephrosis. Study Design and Subjects: Forty-five pregnant lambs were randomly divided into 3 groups. Eighteen lambs were in the hydronephrotic model group. The second group included 9 lambs in which ureteral obstruction was relieved 3 weeks after the initial operation. Eighteen lambs were in the control group which was just performed open hysterotomies. Outcome measures: The amniotic fluid index (AFI) and the expression of neuronspecific enolase (NSE) in the lungs were tested. Results: In the hydronephrotic model group, the AFI was less and the expression of NSE was greater than the control group. The AFI and expression of NSE were similar to the control group after relieving the ureteral obstruction. Conclusions: The AFI and expression of NSE return to normal after after relieving the ureteral obstruction, which meant that relieving ureteral obstruction is perhaps beneficial to lung development.
- A. Becker and M. Baum, “Obstructive Uropathy,” Early Human Development, Vol. 82, No. 1, 2006, pp. 15-22. doi:10.1016/j.earlhumdev.2005.11.002
- F. H. Wang, J. J. Sun, G. Y. Deng and J. C. Mo, “Does Intervention in Utero Preserve the Obstructed Kidneys of Fetal Lambs? A Histological, Cytological, and Molecular Study,” Pediatric Search, Vol. 66, No. 2, 2009, pp. 145-148.
- M. R. Harrison, D. K. Nakayama, R. Noall and A. A. de Lorimier, “Correction of Congenital Hydronephrosis in Utero II, Decompression Reverses the Effects of Obstruction on the Fetal Lung and Urinary Tract,” Journal of Pediatric Surgery, Vol. 17, No. 6, 1982, pp. 965-974. doi:10.1016/S0022-3468(82)80476-4
- T. M. Crombleholme, M. R. Harrison, M. S. Golbus, M. T. Longaker, J. C. Langer, P. W. Callen, R. L. Anderson, R. B. Goldstein and R. A. Filly, “Fetal Intervention in Obstructive Uropathy: Prognostic Indicators and Efficacy of Intervention,” American Journal of Obstetrics and Gynecology, Vol. 162, No. 5, 1990, pp. 1239-1244.
- M. N. Sheppard, P. J. Marangos, S. R. Bloom and J. M. Polak, “Neuron Specific Enolase: A Marker for the Early Development of Nerves and Endocrine Cells in the Human Lung,” Life Science, Vol. 34, No. 3, 1984, pp. 265-271. doi:10.1016/0024-3205(84)90598-8
- K. Asabe, R. W. Jennings, M. R. Harrison and S. Suita, “Quantitative Study of Pulmonary Endocrine Cells in Fetal, Postnatal and Adult Lambs,” Journal of Veterinary Medical Science, Vol. 66, No. 4, 2004, pp. 373-380. doi:10.1292/jvms.66.373
- L. Balaguer and J. Romano, “Solitary Neuroendorine Cells and Neuroepithelial Bodies in the Lower Airways of Embryonic, Fetal, and Postnatal Lambs,” The Anatomical Record, Vol. 231, No. 3, 1991, pp. 333-338. doi:10.1002/ar.1092310306
- D. G. Alcorn, T. M. Adamson, J. E. Maloney and P. M. Robinson, “A Morphologic and Morphometric Analysis of Fetal Lung Development in the Lambs,” Anatomy Research, Vol. 201, No. 4, 1981, pp. 655-667. doi:10.1002/ar.1092010410