HIV-1 Viral Load and CD4 Assessment in HIV-1 Infected Pregnant Women Supported as Part of PMTCT in N’Djamena, Chad
Service de Laboratoire et Médicaments du Programme Sectoriel de Lutte Contre le sida et les Infections Sexuellement Transmissibles, N’djamena, Tchad
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Laboratoire de Microbiologie du CHU Sylvanus OLIMPYO, Département des Sciences Fondamentales, Faculté des Sciences de la Santé, Université de Lomé, Lomé, Togo
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Laboratoire de Microbiologie et de Contrôle de Qualité de Denrées Alimentaires, Ecole Supérieure des Techniques Biologiques et Alimentaires, Université de Lomé, Lomé, Togo
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Service de Prise en Charge des Personnes Vivant avec le VIH/Sida, Centre de l’Appui Psycho Médicosocial, N’djamena, Tchad
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Service de Prise en Charge des Personnes Vivant avec le VIH/Sida, Hôpital Général de Référence National, N’djamena, Tchad
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Département des Sciences Biomédicales et Pharmaceutiques, Institut Universitaire des Sciences et Techniques d’Abéché, Tchad
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Faculté des Sciences de la Santé, Université de N’djamena, N’djamena, Tchad
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Laboratoire de Biologie Moléculaire et D’immunologie, FSS/UL, Lomé, Togo
1 Service de Laboratoire et Médicaments du Programme Sectoriel de Lutte Contre le sida et les Infections Sexuellement Transmissibles, N’djamena, Tchad
2 Laboratoire de Microbiologie du CHU Sylvanus OLIMPYO, Département des Sciences Fondamentales, Faculté des Sciences de la Santé, Université de Lomé, Lomé, Togo
3 Laboratoire de Microbiologie et de Contrôle de Qualité de Denrées Alimentaires, Ecole Supérieure des Techniques Biologiques et Alimentaires, Université de Lomé, Lomé, Togo
4 Service de Prise en Charge des Personnes Vivant avec le VIH/Sida, Centre de l’Appui Psycho Médicosocial, N’djamena, Tchad
5 Service de Prise en Charge des Personnes Vivant avec le VIH/Sida, Hôpital Général de Référence National, N’djamena, Tchad
6 Département des Sciences Biomédicales et Pharmaceutiques, Institut Universitaire des Sciences et Techniques d’Abéché, Tchad
7 Faculté des Sciences de la Santé, Université de N’djamena, N’djamena, Tchad
8 Laboratoire de Biologie Moléculaire et D’immunologie, FSS/UL, Lomé, Togo
In Sub-Saharan Africa, HIV affects lots of women of childbearing age; without prevention they can transmit the virus to their child. A cross-sectional study was conducted in the center of Psycho Medico-Social Support (APMS) in N’Djamena, Chad from January 2014 to March 2015. Our sampling concerned HIV-1 infected pregnant women followed up for PMTCT and their newborn. CD4+ lymphocytes and HIV-1 viral load were tested respectively with PIMATM and Abbott m2000 Real Time in mothers. Early infant diagnosis of HIV-1 was done in Children using PCR tool (Abbott m2000 Real Time). Pregnant women included in the study had a median age of 25 years (IQR, 22 - 30 years). Most of them (75.6%) (34/45), were under combination ART (TDF + 3TC or FTC + EFV). The median duration on ART was 4 month (IQR [3 - 5 months]). Nevirapine syrup was administrated to newborns as prophylaxis at least for the first six weeks of life until EID was done. At ART initiation, mothers’ LTCD4+ median was 249 cells/mm3 (IQR: 95 - 674 cells/mm3). After a median duration of 4 months on ART, LTCD4+ median was 530 cells/mm3 (IQR [263 - 1220 cells/mm3]). Viral load assessment in mothers showed that 15.5% (7/45) were undetectable, 75.6% (34/45) were detectable with a VL < 3log copies/ml and 8.8% (4/45) at virologic failure (VL > 3log copies/ ml). Four (11.4%) of 35 children included were tested positive at EID for HIV-1. Antiretroviral treatment management in pregnant women can improve their health and reduce the risk of MTCT. Availability of virologic monitoring in routine is essential for pregnant women in resources limited setting for preventing HIV transmission to their new-born and keep them alive.
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HIV-1 Viral Load and CD4 Assessment in HIV-1 Infected Pregnant Women Supported as Part of PMTCT in N’Djamena, Chad — Oak Academic Publishing
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