Penicillin Resistance in Nasopharyngeal <i>Streptococcus pneumoniae</i> among Children with Sickle Cell Disease Immunized with 7-Valent Pneumococcal Conjugate Vaccine — Oak Academic Publishing
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Penicillin Resistance in Nasopharyngeal <i>Streptococcus pneumoniae</i> among Children with Sickle Cell Disease Immunized with 7-Valent Pneumococcal Conjugate Vaccine
Pediatric Department, Universidade Federal de S?o Paulo, S?o Paulo, Brazil
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Pediatric Department, Universidade Federal de S?o Paulo, S?o Paulo, Brazil
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Pediatric Department, Universidade Federal de S?o Paulo, S?o Paulo, Brazil
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Department of Infectious Disease, Universidade Federal de S?o Paulo, S?o Paulo, Brazil
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Pediatric Department, Universidade Federal de S?o Paulo, S?o Paulo, Brazil
1 Pediatric Department, Universidade Federal de S?o Paulo, S?o Paulo, Brazil
2 Pediatric Department, Universidade Federal de S?o Paulo, S?o Paulo, Brazil
3 Pediatric Department, Universidade Federal de S?o Paulo, S?o Paulo, Brazil
4 Department of Infectious Disease, Universidade Federal de S?o Paulo, S?o Paulo, Brazil
5 Pediatric Department, Universidade Federal de S?o Paulo, S?o Paulo, Brazil
To assess the prevalence, the antimicrobials resistance and to identify risk factors of nasopharyngeal colonization by Streptococcus pneumoniae in children with sickle cell disease (SCD) using prophylactic penicillin and immunized with 7-valent pneumococcal conjugate vaccine. A total of 424 swabs were collected from the nasopharynx in 216 children with SCD and 109 samples from the control group, both from 2 to 60 months age range. Isolation and identification of pneumococci followed standard procedures. Minimum inhibitory concentration (MIC) for penicillin was determined by the E-test method. Prevalence of nasopharyngeal colonization by pneumococci in children with SCD was 17%, and 11% in the control group. The risk factors for increased colonization in children with SCD were the presence of more than five people at home, daycare/school attendance and low prophylaxis compliance. The prevalence of strains with penicillin resistance was 57.5% in patients with SCD and 25% in the control group. Conclusions: Prevalence of pneumococci nasopharyngeal colonization was similar among the study groups. Prophylactic use of penicillin may have increased the prevalence of resistant strains. The vaccine did not decrease the colonization with penicillin resistant strains.
H. A. Pearson, “Sickle Cell Anemia and Serious Infections Due to Encapsulated Bacteria,” Journal of Infectious Disease, Vol. 136, Suppl. 1, 1977, pp. S25-S30. doi:10.1093/infdis/136.Supplement.S25
M. H. Gaston, J. I. Verter, G. Woods, C. Pegelow, J. Kelleher, G. Presbury, H. Zarkowsky, E. Vichinsky, R. Iyer, et al., “Prophylaxis with Oral Penicillin in Children with Sickle Cell Anemia: A Randomized Trial,” New England Journal of Medicine, Vol. 314, No. 25, 1986, pp. 1593-1599. doi:10.1056/NEJM198606193142501
M. da Saúde do Brasil, “Manual dos Centros de Referência para Imunobiológicos Especiais. Organizado pela Coordenacoo de Imunizacoes e Auto-Suficiência em Imunobiológicos,” Ministério da Saúde, Secretaria de Vigilancia à Saúde, Programa Nacional de Imunizacao 2005, Brasília, 2006, 150 p.?
M. C. C. Brandileone, A. L. S. Andrade, J. L. Di Fabio, M. L. L. S. Guerra and R. Austrian, “Appropriateness of a Pneumococcal Conjugate Vaccine in Brazil: Potential Impact of Age and Clinical Diagnosis, with Emphasis on Meningitis,” Journal of Infectious Disease, Vol. 187, No. 8, 2003, pp.1206-1212. doi:10.1086/374396
G. D. Overturf, R. Field, C. Lam, S. Lee and D. R. Powars, “Nasopharyngeal Carriage of Pneumococci in Children with Sickle Cell Disease,” Infection and Immunity, Vol. 28, No. 3, 1980, pp. 1048-1050.
P. B. Fonseca, J. A. Braga, A. M. Machado, M. C. C. Brandileone and C. K. Farhat, “Colonizacao Nasofaríngea pelo Streptococcus pneumoniae em Crian?as com Doen?a Falciforme Usando Penicilina Profilática,” Jornal de Pediatria, Vol. 81, No. 2, 2005, pp. 149-154. doi:10.1590/S0021-75572005000300011?
F. Ghaffar, T. Barton, J. Lozano, L. S. Muniz, P. Hicks, V. Gan, N. Ahmad and G. H. McCracken Jr., “Effect of 7-Valent Pneumococcal Conjugate Vaccine on Nasopharyngeal Colonization by Streptococcus pneumonia in the First 2 Years of Life,” Clinical Infectious Diseases, Vol. 39, No. 7, 2004, pp. 930-938. doi:10.1086/423379
V. F. Jones, C. Harrison, G. G. Stout and J Hopkins, “Nasopharyngeal Colonization with Heptavalent Pneumococcal Conjugate Vaccine Serotypes of Streptococcus pneumonia with Prolonged Vaccine Dosing Intervals,” Pediatric Infectious Diseases Journal, Vol. 24, No. 11, 2005, pp. 969-973. doi:10.1097/01.inf.0000187030.83080.8a
C. Booth, B. Inusa and S. K. Obaro, “Infection in Sickle Cell Disease: A Review,” International Journal of Infectious Diseases, Vol. 14, No. 1, 2010, pp. e2-e12. doi:10.1016/j.ijid.2009.03.010
T. M. McCavit, C. T. Quinn, C. Techasaensiri and Z. R. Rogers, “Increase in Invasive Streptococcus pneumoniae Infections in Children with Sickle Cell Disease Since Pneumococcal Conjugate Vaccine Licensure,” The Journal of Pediatrics, Vol. 158, No. 3, 2011, pp. 505-507. doi:10.1016/j.jpeds.2010.11.025
V. S. Sakhalkar, A. S. Sarnaik, B. I. Asmar, R. Conner-Warren, W. Shurney and N. M. Abdel-Haq, “Prevalence of Penicillin-Nonsusceptible Streptococcus pneumoniae in Nasopharyngeal Cultures from Patients with Sickle Cell Disease,” Southern Medical Journal, Vol. 94, No. 4, 2001, pp. 401-404.
S. J. Schrag, C. Pena, J. Fernández, J. Sánchez, V. Gómez, E. Pérez, J. M. Feris and R. E. Besser, “Effect of Short-Course, High-Dose Amoxicillin Therapy on Resistant Pneumococcal Carriage: A Randomized Trial,” The Journal of the American Medical Association, Vol. 286, No. 1, 2001, pp. 49-56. doi:10.1001/jama.286.1.49?
M. H. Kyaw, R. Lynfield, W. Schaffner, A. S. Craig, J. Hadler, A. Reingold, A. R. Thomas, L. H. Harrison, N. M. Bennett, M. M. Farley, R. R.Facklam, J. H. Jorgensen, J. Besser, E. R. Zell, A. Schuchat and C. G. Whitney, “Effect of Introduction of the Pneumococcal Conjugate Vaccine on Drug-Resistant Streptococcus pneumonia,” The New England Journal of Medicine, Vol. 354, No. 14, 2006, pp. 1455-1463. doi:10.1056/NEJMoa051642
R. Dagan, “Impact of Pneumococcal Conjugate Vaccine on Infections Caused by Antibiotic-Resistant Streptococcus pneumonia,” Clinical Microbiology and Infection, Vol. 3, No. 1, 2009, pp. 16-20. doi:10.1111/j.1469-0691.2009.02726.x
Clinical and Laboratory Standards Institute, “Performance Standards for Antimicrobial Susceptibility Testing,” Eighteenth Informational Supplement, CLSI Publication M100-S18, Vol. 28, No. 1, 2008.
D. L. Anglin, J. D. Siegel, D. L. Pacini, S. J. Smith, G. Adams and G. R. Buchanan, “Effect of Penicillin Prophylaxis on Nasopharyngeal Colonization with Streptococcus pneumoniae in Children with Sickle Cell Anemia,” Journal of Pediatrics, Vol. 104, No. 1, 1984, pp. 18-22. doi:10.1016/S0022-3476(84)80582-X
C. F. Norris, S. R. Mahannah, K. Smith-Witley, K. O. Frempong and K. L. McGowan, “Pneumococcal Colonization in Children with Sickle Cell Disease,” Journal of Pediatrics, Vol. 129, No. 6, 1996, pp. 821-827. doi:10.1016/S0022-3476(96)70025-2
G. M. Woods, J. H. Jorgensen, M. A. Waclawiw, C. Reid, W. Wang, C. H. Pegelow, Z. R. Rogers, R. V. Iyer, C. T. Holbrook, T. R. Kinney, E. Vichinsky, M. R. DeBaun, N. J. Grossman, M. D. Thomas and J. M. Falletta, “Influence of Penicillin Prophylaxis on Antimicrobial Resistance in Nasopharyngeal S. pneumoniae among Children with Sickle Cell Anemia,” Journal of Pediatric Hematology/ Oncology, Vol. 19, No. 4, 1997, pp. 327-333. doi:10.1097/00043426-199707000-00011
E. Alexander, P. Telfer, H. Rashid, K. A. Ali and R. Booy, “Nasopharyngeal Carriage Rate of Streptococcus pneumoniae in Children with Sickle Cell Disease before and after the Introduction of Heptavalent Pneumococcal Conjugate Vaccine,” Journal of Infection and Public Health, Vol. 1, No. 1, 2008, pp. 40-44. doi:10.1016/j.jiph.2008.08.005
N. Fraz?o, R. Sá-Le?o and H. Lencastre, “Impact of a Single Dose of the 7-Valent Pneumococcal Conjugate Vaccine on Colonization,” Vaccine, Vol. 28, No. 19, 2010, pp. 3445-3452. doi:10.1016/j.vaccine.2010.02.070
L. C. Rey, B. Wolf, L. B. Moreira, J. Verhoef and C. K. Farhat, “S. pneumoniae Isolados da Nasofaringe de Crian?as Sadias e com Pneumonia: Taxa de Coloniza??o e Suscetibilidade aos Antimicrobianos,” Jornal de Pediatria, Vol. 78, No. 2, 2002, pp. 105-112. doi:10.1590/S0021-75572002000200008
M. G. M. Novales, F. S. Snatos, H. G. Gallardo, B. L. Miranda, G. E. Aviles, M. N. C. Barajas, M. P. Torres and O. M. Hermandez, “Streptococcus pneumoniae: Low Frequency of Penicillin Resistance and High Resistance to Trimetoprim-Sulfamethoxazole in Nasopharyngeal Isolates from Children in a Rural Area in Mexico,” Archives of Medical Research, Vol. 28, No. 4, 1997, pp. 559-563.
A. Woolfson, R. Huebner, A. Wasas, M. Chola, P. Godfrey-Faussett and K. Klugman, “Nasopharyngeal Carriage of Community-Acquired-Antibiotic-Resistant Streptococcus pneumoniae in a Gambian Pediatric Population,” Bulletin of the World Health Organization, Vol. 75, No. 5, 1997, pp. 453-462.
P. A. Lane, J, L. O’Connel, J. L Lear, Z. R. Rogers, G. M. Woods, K. L. Hassell, D. L. Wethers, D. W. Luckey and G. R. Buchanan, “Functional Asplenia in Hemoglobin SC Disease,” Blood, Vol. 85, No. 8, 1995, pp. 2238-2244.
J. Melo-Cristino, M. Ramirez, N. Serrano and T. H?nscheid, “Macrolide Resistance in Streptococcus pneumoniae Isolated from Patients with Community-Acquired Lower Respiratory Tract Infections in Portugal: Results of a 3-Year (1999-2001) Multicenter Surveillance Study,” Microbial Drug Resistance, Vol. 9, No. 1, 2003, pp. 73-80. doi:10.1089/107662903764736364
K. Waits and S. Brown, “Antimicrobial Resistance among Isolates of Respiratory Tract Infection Pathogens from the Southern United States: Data from the PROTEKT US Surveillance Program 2000/2001,” Southern Medical Journal, Vol. 96, No. 10, 2003, pp. 974-985. doi:10.1097/01.SMJ.0000091066.74656.6C
A. D. Conradi, E. Calbo, E. Cuchí, et al., “Spanish Pneumococcal Infection Study Network, Impact of Amoxicillin, Associated or Not with Clavulanic Acid, on Pharyngeal Colonization and Selection of Streptococcus pneumoniae Resistance in Children under 5 Years of Age,” European Journal of Pediatrics, Vol. 166, No. 5, 2007, pp. 467-471. doi:10.1007/s00431-006-0270-5