Evaluation of “Indigenous Vaccine” Developed Using “Indian Bison Type” Genotype of <i>Mycobacterium avium subspecies paratuberculosis</i> Strain “S5” of Goat Origin in a Sheep Flock Endemic for Johne’s Disease: A Three Years Trial in India

Abstract

“ Indigenous vaccine ” developed from native “ Indian Bison Type ” strain ( “ S5”) of Mycobacterium avium subspecies paratuberculosis (MAP) of “ goat origin ” was first time evaluated in a sheep flock of Bharat Merino breed located in Mannavanur town of Tamil Nadu in South India. Therapeutic efficacy of the vaccine was evaluated for 3 years between 2008 and 2010, on the basis of improvements in productivity (body weights, reproductive efficiency and survivability), physical condition, clinical symptoms (weakness, diarrhea, wool quality), immune response (sero-conversion) and infection load in feces (shedding). After immunization of the flock in 2008, the successive progenies of 112 and 53 lambs born in 2009 and 2010, respectively were vaccinated. Whereas, 40 lambs born to control animals were kept as unvaccinated controls. Though gain in body weights in vaccinated versus controls were not significant in 2008, growth rates were distinctly superior in I st and II nd generations of vaccinated lambs. Reproductive performance (tupping percent) and survivability of lambs and adult sheep impr oved significantly. There was overall reduction in yearly mor bidity (diarrhea) and mortality rates of the flock in post vaccination years. Shedding of MAP in feces was reduced in vaccinated sheep by 6.2%, 14.3% and 27.3% in 2008, 2009 and 2010 respectively , whereas shedding increased in the control sheep. Sero monitoring of the animals by “ indigenous ELISA kit ” showed enhanced “ flock immunity ” in successive generations. “ Indigenous vaccine ” reduced clinical disease and shedding and improved immunity and productivity of Bharat Merino flock, endemic for Johne’s disease.