This study observed the immune response induced by a HSP65 DNA vaccine fused with UbGR against Mycobacterium tuberculosis . BALB/c mice were inoculated with HSP65 DNA vaccine, UbGR-fused HSP65 DNA vaccine (Ub - GR-HSP65) and blank vector respectively. HSP65 DNA vaccine elicited a Th l -polarized immune response. The Th l -type cytokine (IFN- γ ) and proliferative T cell responses from spleen were improved significantly in UbGR-HSP65 group, compared with th ose in HSP65 DNA vaccine group. Furthermore, this fusion DNA vaccine also le d to an in creased ratio of IgG 2a to IgG l and the cytotoxicity of T cells. IFN- γ intracellular staining of splenocytes indicated that UbGR-HSP65 fusion DNA vaccine could activate CD4 + and CD8 + T cells, with much higher CD8 + T cells. Thus, this study demonstrated that the UbGR fusion could improve HSP65-specific cellular immune responses , which is helpful to protect against TB infection.
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