Research ArticleOpen AccessGoogle Scholar indexed
Human CD4<sup>-</sup> CD8<sup>-</sup> Invariant Natural Killer T Cells Promote IgG Secretion from B Cells Stimulated by Cross-Linking of Their Antigen Receptors
Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan
Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan
Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan
Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan
Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan
Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Tokyo, Japan
Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Tokyo, Japan
Division of Immunology, Aichi Cancer Center Research Institute (ACCRI), Nagoya, Japan
Key Laboratory of Cancer Center, Chinese PLA General Hospital, Beijing, China
Division of Immunology, Aichi Cancer Center Research Institute (ACCRI), Nagoya, Japan
Division of Cancer Immunotherapy, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center (NCC), Kashiwa, Japan
Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan
- 1 Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan
- 2 Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan
- 3 Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan
- 4 Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan
- 5 Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan
- 6 Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Tokyo, Japan
- 7 Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Tokyo, Japan
- 8 Division of Immunology, Aichi Cancer Center Research Institute (ACCRI), Nagoya, Japan
- 9 Key Laboratory of Cancer Center, Chinese PLA General Hospital, Beijing, China
- 10 Division of Immunology, Aichi Cancer Center Research Institute (ACCRI), Nagoya, Japan
- 11 Division of Cancer Immunotherapy, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center (NCC), Kashiwa, Japan
- 12 Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan
World Journal of Vaccines·Volume 06 (2016)·Pages 34–41·Published 12 May 2016·DOI10.4236/wjv.2016.62005
Copy link · social · email
Abstract
Immunoglobulin (Ig) M production can be induced by the interaction of thymus-independent type-2 (TI-2) antigen (Ag) with B cell Ag receptors (BCRs) without the involvement of conventional T cells; for IgG production through the same process, however, a second signal is required. Previous studies have reported that invariant natural killer T (iNKT) cells may be responsible for the second signal involved in IgG production. In the present study, we addressed whether human iNKT cells could participate in the production of Ig against TI-2 Ag in vitro. Two major distinct subsets of human iNKT cells, CD4 + CD8β - (CD4) and CD4 - CD8β - [double negative (DN)] cells, were generated from peripheral blood monocytes from a healthy volunteer. BCR engagement, triggered by anti-IgM antibody stimulation, examined here as a model of BCR engagement triggered by TI-2 Ag, induced abundant IgM production by B cells. Both CD4 and DN iNKT cells reduced IgM production and conversely enhanced IgG production in a dose-dependent manner. In addition, IgG production by CD19 + CD27 - (naïve) and CD19 + CD27 + (memory) B cells was predominantly promoted by DNiNKT cells rather than CD4 iNKT cells; nevertheless, IgM production by both B cell subsets was similarly reduced by either subset of iNKT cells. These results suggest that the DN iNKT subsets may preferentially promote Ig class switching by B cells upon stimulation with TI-2 Ag.
KeywordsInvariant Natural Killer T CellsTI-2 AntigenB CellsIgMIgG
- De Velasco, E.A., Verheul, A.F., Verhoef, J. and Snippe, H. (1995) Streptococcus pneumoniae: Virulence Factors, Pathogenesis, and Vaccines. Microbiology Reviews, 59, 591-603.
- Vitharsson, G., Jónsdóttir, I., Jónsson, S. and Valdimarsson, H. (1994) Opsonization and Antibodies to Apsular and Cell Wall Polysaccharides of Streptococcus pneumoniae. The Journal of Infectious Diseases, 170, 592-599. http://dx.doi.org/10.1093/infdis/170.3.592
- Hallström, T. and Riesbeck, K. (2010) Haemophilus influenzae and the Complement System. Trends in Microbiology, 18, 258-265. http://dx.doi.org/10.1016/j.tim.2010.03.007
- Miyasaka, T., Akahori, Y., Toyama, M., Miyamura, N., Ishii, K., Saijo, S., Iwakura, Y., Kinjo, Y., Miyazaki, Y., Oishi, K. and Kawakami K. (2013) Dectin-2-Dependent NKT Cell Activation and Serotype-Specific Antibody Production in Mice Immunized with Pneumococcal Polysaccharide Vaccine. PLoS One, 8, e78611. http://dx.doi.org/10.1371/journal.pone.0078611
- Miyasaka, T., Aoyagi, T., Uchiyama, B., Oishi, K., Nakayama, T., Kinjo, Y., Miyazaki, Y., Kunishima, H., Hirakata, Y., Kaku, M. and Kawakami, K. (2012) A Possible Relationship of Natural Killer T Cells with Humoral Immune Response to 23-Valent Pneumococcal Polysaccharide Vaccine in Clinical Settings. Vaccine, 30, 3304-3310. http://dx.doi.org/10.1016/j.vaccine.2012.03.007
- Grabenstein, J.D. and Manoff, S.B. (2012) Pneumococcal Polysaccharide 23-Valent Vaccine: Long-Term Persistence of Circulating Antibody and Immunogenicity and Safety after Revaccination in Adults. Vaccine, 30, 4435-4444. http://dx.doi.org/10.1016/j.vaccine.2012.04.052
- Mond, J.J., Lees, A. and Snapper, C.M. (1995) T Cell-Independent Antigens Type 2. Annual Review of Immunology, 13, 655-692. http://dx.doi.org/10.1146/annurev.iy.13.040195.003255
- Snapper, C.M., Rosas, F., Moorman, M.A., Jin, L., Shanebeck, K., Klinman, D.M., Kehry, M.R., Mond, J.J. and Maliszewski, C.R. (1996) IFN-Gamma Is a Potent Inducer of Ig Secretion by Sort-Purified Murine B Cells Activated through the mIg, but not the CD40, Signaling Pathway. International Immunology, 8, 877-885. http://dx.doi.org/10.1093/intimm/8.6.877
- Vos, Q., Lees, A., Wu, Z.Q., Snapper, C.M. and Mond, J.J. (2000) B-Cell Activation by T-Cell-Independent Type 2 Antigens as an Integral Part of the Humoral Immune Response to Pathogenic Microorganisms. Immunological Reviews, 176, 154-170. http://dx.doi.org/10.1034/j.1600-065X.2000.00607.x
- Snapper, C.M., McIntyre, T.M., Mandler, R., Pecanha, L.M., Finkelman, F.D., Lees, A. and Mond, J.J. (1992) Induction of IgG3 Secretion by Interferon Gamma: A Model for T Cell-Independent Class Switching in Response to T Cell-Independent Type 2 Antigens. The Journal of Experimental Medicine, 175, 1367-1371. http://dx.doi.org/10.1084/jem.175.5.1367