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ABB is an international journal dedicated to the latest advancements in biosciences. The goal of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of biosciences. All manuscripts must be prepare…
Magnus Sjöblom, Linda Lindberg, Jan Holgersson, Ulrika Rova
The methanol inducible alcohol oxidase 1 promoter and the Saccharomyces cerevisiae alpha-factor prepro secretion signal were used to drive expression and secretion of a mucin-type fusion protein by Pichia pastoris in 1 L scale bioreactors. The aim of the stud…
Junming Bai, Ruiwen Fan, Shanshan Yang, Yuankai Ji, Jianshan Xie, Chang-Sheng Dong
Ribosomal proteins (RP) has been reported as a central player in the translation system, and are required for the growth and maintenance of all cell kinds. RP genes form a family of homologous proteins that express in the stable pattern and were used for phyl…
Isaac Olusanjo Adewale, Olanrewaju Roland Akinseye, Adekanbi Idowu Odutayo, Isaac Olusanjo Adewale
We present a preliminary report on the use of plant dyes in the quantitation of proteins in solution. We have used ethanol, acid, alkali and water to extract dyes from some plant materials, including flowers of Jungle flame ( Izora coccinea ), China rose ( Hi…
Rajeev Nema, Sarita Khare
At the present time animal cell culture is more significant and multifarious application tool for current research streams. A lot of field assorted from animal cell culture such: stem cell biology, IVF technology, cancer cell biology, monoclonal antibody prod…
Houli Jiang, Fiona E. Harrison, Kavita Jain, Samantha Benjamin, James M. May, Joan P. Graves, Darryl C. Zeldin, John R. Falck, Bruce D. Hammock, John C. McGiff
The cardiovascular effects of vitamin C (VitC) could be mediated by epoxyeicosatrienoic acids (EETs). We aimed to study the mechanism of VitC-dependent microsomal formation of cis - and trans -EETs and the regulation of EET levels in rat isolated perfused kid…
Gang Wang, Ka-Bik Lai, Bonnie Ching-Ha Kwa, Kai-Ming Chow, Philip Kam-Tao Li, Cheuk-Chun Szeto
Background: Cell-free microRNAs (miRNAs) exist in body fluid. Previous studies showed that cell-free mi-RNAs are partly bound in microvesicles, and could transfer between cells via fusion with cell membrane. Methods: We quantified the amount of a panel of mi-…
Kholmirzo Kholmurodov, Ermuhammad Dushanov, Kenji Yasuoka
A molecular dynamics (MD) simulation is performed on a DNA photolyase to study the conformational behavior of the photoactive cofactor flavin adenine dinucleotide (FAD) inside the enzyme pocket. A DNA photolyase is a highly efficient light-driven enzyme that…
Roswanira Abdul Wahab, Mahiran Basri, Mohd Basyaruddin Abdul Rahman, Raja Noor Zaliha Raja Abdul Rahman, Abu Bakar Salleh, Leow Thean Chor
In-silico and experimental investigations were conducted to explore the effects of substituting hydrophobic residues; Val, Met, Leu, Ile, Trp and Phe into the oxyanion Q114 of T1 lipase. We hypothesized that the oxyanion Q114, involved in substrate binding is…
Xueyan Lin, Zhonghua Wang, Zhongxiang Niu, Jun Peng, Yun Wang
Lactobacillus adheres to intestinal epithelial cells and yeast fungus cells with the aid of adhesion factors expressed on its cell surface. To identify adhesion factors nature on the surface of Lactobacillus , an adhesion experiment was carried out by pre-tre…
Xueyan Lin, Zhonghua Wang, Zhongxiang Niu, Hongjie Liu, Yun Wang
Adhesive ability was tested in seven Lactobacillus solated from the chicken digestive tract after cultivation with CaCo-2 cells (intestinal epithelial cells), MDCK (dog kidney) cells and CEF (chicken embryo fiber) cells. We noted the following important obser…
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ABB is an international journal dedicated to the latest advancements in biosciences. The goal of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of biosciences. All manuscripts must be prepare… All articles are open access under a CC BY 4.0 licence, with authors retaining copyright.
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